Real-time PCR for sexing Schistosoma mansoni cercariae

Detalhes bibliográficos
Autor(a) principal: Chevalier, Frédéric D.
Data de Publicação: 2016
Outros Autores: Clec’h, Winka Le, Mattos, Ana Carolina Alves de, LoVerde, Philip T., Anderson, Timothy J.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53594
Resumo: Department of Genetics. Texas Biomedical Research Institute. San Antonio, TX, USA
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spelling Chevalier, Frédéric D.Clec’h, Winka LeMattos, Ana Carolina Alves deLoVerde, Philip T.Anderson, Timothy J.C.2022-06-30T16:07:05Z2022-06-30T16:07:05Z2016CHEVALIER, Frédéric D. et al. Real-time PCR for sexing Schistosoma mansoni cercariae. Mol Biochem Parasitol., v. 205, n. 1-2, p. 35–38, 2016. doi: 10.1016/j.molbiopara.2016.03.0100166-6851https://www.arca.fiocruz.br/handle/icict/5359410.1016/j.molbiopara.2016.03.010engElsevier/North-Holland Biomedical PressReal-time PCR for sexing Schistosoma mansoni cercariaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Genetics. Texas Biomedical Research Institute. San Antonio, TX, USADepartment of Genetics. Texas Biomedical Research Institute. San Antonio, TX, USADepartments of Biochemistry and Pathology. University of Texas Health Science Center. San Antonio, TX, USA/Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, BrasilDepartments of Biochemistry and Pathology. University of Texas Health Science Center. San Antonio, TX, USADepartment of Genetics. Texas Biomedical Research Institute. San Antonio, TX, USAThe gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end point PCR can be incorrect, we quantified the W6 repeat sequence and a specific Z chromosome gene using real-time PCR. The ratio between copy number of W6 and a Z chromosome marker unambiguously identifies gender: females have higher ratios (421-4371) than males (0-21). However, some males have low numbers of W6 elements in their genome, and qPCR demonstrated significantly higher W6/Z marker ratios for male genotypes giving ambiguous end point PCR results compared with males giving clear end point results. The quantitative PCR sexing method developed will be particularly useful where reliable sexing of cercariae is critical, for example when staging genetic crosses.CercariaeSchistosomeSexing methodsqPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/53594/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALReal-time PCR for sexing Schistosoma mansoni cercariae - nihms776578.pdfReal-time PCR for sexing Schistosoma mansoni cercariae - nihms776578.pdfapplication/pdf267381https://www.arca.fiocruz.br/bitstream/icict/53594/2/Real-time%20PCR%20for%20sexing%20Schistosoma%20mansoni%20cercariae%20-%20nihms776578.pdfc590c959ef29395712390185368a50dbMD52icict/535942022-06-30 13:07:05.464oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-30T16:07:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Real-time PCR for sexing Schistosoma mansoni cercariae
title Real-time PCR for sexing Schistosoma mansoni cercariae
spellingShingle Real-time PCR for sexing Schistosoma mansoni cercariae
Chevalier, Frédéric D.
Cercariae
Schistosome
Sexing methods
qPCR
title_short Real-time PCR for sexing Schistosoma mansoni cercariae
title_full Real-time PCR for sexing Schistosoma mansoni cercariae
title_fullStr Real-time PCR for sexing Schistosoma mansoni cercariae
title_full_unstemmed Real-time PCR for sexing Schistosoma mansoni cercariae
title_sort Real-time PCR for sexing Schistosoma mansoni cercariae
author Chevalier, Frédéric D.
author_facet Chevalier, Frédéric D.
Clec’h, Winka Le
Mattos, Ana Carolina Alves de
LoVerde, Philip T.
Anderson, Timothy J.C.
author_role author
author2 Clec’h, Winka Le
Mattos, Ana Carolina Alves de
LoVerde, Philip T.
Anderson, Timothy J.C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chevalier, Frédéric D.
Clec’h, Winka Le
Mattos, Ana Carolina Alves de
LoVerde, Philip T.
Anderson, Timothy J.C.
dc.subject.en.pt_BR.fl_str_mv Cercariae
Schistosome
Sexing methods
qPCR
topic Cercariae
Schistosome
Sexing methods
qPCR
description Department of Genetics. Texas Biomedical Research Institute. San Antonio, TX, USA
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2022-06-30T16:07:05Z
dc.date.available.fl_str_mv 2022-06-30T16:07:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHEVALIER, Frédéric D. et al. Real-time PCR for sexing Schistosoma mansoni cercariae. Mol Biochem Parasitol., v. 205, n. 1-2, p. 35–38, 2016. doi: 10.1016/j.molbiopara.2016.03.010
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53594
dc.identifier.issn.pt_BR.fl_str_mv 0166-6851
dc.identifier.doi.none.fl_str_mv 10.1016/j.molbiopara.2016.03.010
identifier_str_mv CHEVALIER, Frédéric D. et al. Real-time PCR for sexing Schistosoma mansoni cercariae. Mol Biochem Parasitol., v. 205, n. 1-2, p. 35–38, 2016. doi: 10.1016/j.molbiopara.2016.03.010
0166-6851
10.1016/j.molbiopara.2016.03.010
url https://www.arca.fiocruz.br/handle/icict/53594
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier/North-Holland Biomedical Press
publisher.none.fl_str_mv Elsevier/North-Holland Biomedical Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53594/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/53594/2/Real-time%20PCR%20for%20sexing%20Schistosoma%20mansoni%20cercariae%20-%20nihms776578.pdf
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