In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract

Detalhes bibliográficos
Autor(a) principal: Chiavini, Mariê Scotegagna
Data de Publicação: 2019
Outros Autores: Gelinski, Jane Mary Lafayette Neves, Locatelli, Claudriana, Costa, Pâmela Aparecida da, Vicente, Vânia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39991
Resumo: Universidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.
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spelling Chiavini, Mariê ScotegagnaGelinski, Jane Mary Lafayette NevesLocatelli, ClaudrianaCosta, Pâmela Aparecida daVicente, Vânia Aparecida2020-02-17T11:46:37Z2020-02-17T11:46:37Z2019CHIAVINI, Mariê Scotegagna et al. In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract. Revista Fitos, Rio de Janeiro, v. 13, n. 4, p. 278-288, 2019.1808-9569https://www.arca.fiocruz.br/handle/icict/3999110.32712/2446-4775.2019.7922446-4775Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde.In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extractinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.Universidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.Universidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.Universidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Patologia Básica. Curitiba, PR, Brasil.The antimicrobial potential of cranberry hydro alcoholic extracts (CrE) was evaluated against Escherichia coli isolated from women with urinary tract infection (UTI). CrE was diluted based on the percentage of proanthocyanidins (PACs) in extract powder for final concentrations: 1.26%; 2.52%; 3.35%, 5.03% and 10.06%. CrE antimicrobial potential was evaluated by disk and well diffusion assays, and by in vitro direct action against E. coli. Antibacterial action was observed for all performed tests: the minimal inhibitory concentration (MIC) was 1.26% PACs per disk diffusion assay and 2.52% of PACs by well diffusion assay. The in vitro antimicrobial direct action against E. coli resulted 3.8 Log10 cycles reduction for a concentration of 5.03% of PACs. One of the isolates showed multi resistance to antibiotics.  but it was also inhibited more than any of the antibiotic tested in well diffusion assay. Only for concentrations 1.26%, 2.52% and 3.45% the inhibition of Escherichia coli by cranberry extract was dose-dependent, i.e directly proportional to the concentration of PACs. The results indicate a high potential for inhibitory action of CrE. However, more in vitro and in vivo analysis can be performed to fix which the best concentration of CrE capable of causing a real beneficial effect on UTI´s.UTIProanthocyanidinsPharmaceutical PlantVaccinium Macrocarponinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/39991/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALmarie_scotegagna_et_all.pdfapplication/pdf656351https://www.arca.fiocruz.br/bitstream/icict/39991/2/marie_scotegagna_et_all.pdf2fc0f775a1811f006f22f747f09eb7d9MD52TEXTmarie_scotegagna_et_all.pdf.txtmarie_scotegagna_et_all.pdf.txtExtracted texttext/plain35087https://www.arca.fiocruz.br/bitstream/icict/39991/3/marie_scotegagna_et_all.pdf.txtbe7bfd88c68461bf9f50a9ea7b38c1d3MD53icict/399912020-03-25 22:51:03.58oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-03-26T01:51:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
title In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
spellingShingle In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
Chiavini, Mariê Scotegagna
UTI
Proanthocyanidins
Pharmaceutical Plant
Vaccinium Macrocarpon
title_short In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
title_full In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
title_fullStr In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
title_full_unstemmed In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
title_sort In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract
author Chiavini, Mariê Scotegagna
author_facet Chiavini, Mariê Scotegagna
Gelinski, Jane Mary Lafayette Neves
Locatelli, Claudriana
Costa, Pâmela Aparecida da
Vicente, Vânia Aparecida
author_role author
author2 Gelinski, Jane Mary Lafayette Neves
Locatelli, Claudriana
Costa, Pâmela Aparecida da
Vicente, Vânia Aparecida
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chiavini, Mariê Scotegagna
Gelinski, Jane Mary Lafayette Neves
Locatelli, Claudriana
Costa, Pâmela Aparecida da
Vicente, Vânia Aparecida
dc.subject.en.pt_BR.fl_str_mv UTI
Proanthocyanidins
Pharmaceutical Plant
Vaccinium Macrocarpon
topic UTI
Proanthocyanidins
Pharmaceutical Plant
Vaccinium Macrocarpon
description Universidade do Oeste de Santa Catarina. Núcleo Biotecnológico. Videira, SC, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-17T11:46:37Z
dc.date.available.fl_str_mv 2020-02-17T11:46:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHIAVINI, Mariê Scotegagna et al. In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract. Revista Fitos, Rio de Janeiro, v. 13, n. 4, p. 278-288, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39991
dc.identifier.issn.pt_BR.fl_str_mv 1808-9569
dc.identifier.doi.pt_BR.fl_str_mv 10.32712/2446-4775.2019.792
dc.identifier.eissn.none.fl_str_mv 2446-4775
identifier_str_mv CHIAVINI, Mariê Scotegagna et al. In vitro inhibition of Escherichia coli from women with urinary tract infection by cranberry hydroalcoholic extract. Revista Fitos, Rio de Janeiro, v. 13, n. 4, p. 278-288, 2019.
1808-9569
10.32712/2446-4775.2019.792
2446-4775
url https://www.arca.fiocruz.br/handle/icict/39991
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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