Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46002 |
Resumo: | KFH (Wellcome Trust, UK, WT084273/Z/07/Z) and JJC (National Institutes of Health (NIH), USA, 1R01AI121037). PSGF was supported by the Brazilian Government’s Science Without Borders scheme. |
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Perally, SamirahGeyer, Kathrin K.Farani, Priscila S.G.Chalmers, Iain W.Fuentes, Narcis FernandezMaskell, Daniel R.Hulme, Benjamin J.Thomas, Josephine FordePhillips, DylanFarias, Leonardo P.Collins, James J.Hoffmann, Karl F.2021-02-09T12:22:46Z2021-02-09T12:22:46Z2020PERALLY, Samirah et al. Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function. International Journal for Parasitology, 2020.0020-7519https://www.arca.fiocruz.br/handle/icict/4600210.1016/j.ijpara.2020.09.004KFH (Wellcome Trust, UK, WT084273/Z/07/Z) and JJC (National Institutes of Health (NIH), USA, 1R01AI121037). PSGF was supported by the Brazilian Government’s Science Without Borders scheme.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.University of Texas Southwestern Medical Center. Department of Pharmacology. Dallas, TX, USA.Aberystwyth University. Institute of Biological, Environmental and Rural Sciences. Aberystwyth, United Kingdom.The Schistosoma mansoni venom allergen-like protein (SmVAL) superfamily is a collection of at least 29 molecules that have been classified into two distinctive groups (Group 1 and Group 2 SmVALs). The fundamental basis for SmVAL segregation relates to signal peptide and conserved cysteine retention (present in all Group 1 SmVALs, but absent in all Group 2 SmVALs). These structural differences have led to the hypothesis that most Group 1 SmVALs, found as components of schistosome excretory/secretory (E/S) products, predominantly interact with their environment (intermediate or definitive hosts) whereas the Group 2 SmVALs are retained within the schistosome to fulfil parasite-related functions. While experimental evidence to support Group 1 SmVAL/host interactions is growing, similar support for identification of parasite-related Group 2 SmVAL functions is currently lacking. By applying a combination of approaches to the study of SmVAL6, we provide the first known evidence for an essential function of a Group 2 SmVAL in schistosome biology. After whole mount in situ hybridisation (WISH) localised Smval6 to the anterior region of the oesophageal gland (AOG) and cells scattered through the mesenchyme in adult schistosomes, short interfering RNA (siRNA)-mediated silencing of Smval6 was employed to assess loss of function phenotypes. Here, siSmval6-mediated knockdown of transcript and protein levels led to an increase in tegumental permeability as assessed by the quantification of TAMRA-labelled dextran throughout sub-tegumental cells/tissues. Yeast two hybrid screening using SmVAL6 as a bait revealed Sm14 (a fatty acid binding protein) and a dynein light chain (DLC) as directly interacting partners. Interrogation of single-cell RNA-seq (scRNA-seq) data supported these protein interactions by demonstrating the spatial co-expression of Smval6/dlc/Sm14 in a small proportion of adult cell types (e.g. neurons, tegumental cells and neoblasts). In silico modelling of SmVAL6 with Sm14 and DLC provided evidence that opposing faces of SmVAL6 were likely responsible for these protein/protein interactions. Our results suggest that SmVAL6 participates in oesophageal biology, formation of higher order protein complexes and maintenance of tegumental barrier function. Further studies of other Group 2 SmVALs may reveal additional functions of this enigmatic superfamily.engElsevierVenenoSchistosoma mansoniTegumentoVenom allergen-likeSchistosoma mansoniTegumentSchistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier functioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46002/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPerally, Samirah Schistosoma mansoni....pdfPerally, Samirah Schistosoma mansoni....pdfapplication/pdf2880231https://www.arca.fiocruz.br/bitstream/icict/46002/2/Perally%2c%20Samirah%20Schistosoma%20mansoni....pdff14b8fbe816efd39224961333f546405MD52TEXTPerally, Samirah Schistosoma mansoni....pdf.txtPerally, Samirah Schistosoma mansoni....pdf.txtExtracted texttext/plain56751https://www.arca.fiocruz.br/bitstream/icict/46002/3/Perally%2c%20Samirah%20Schistosoma%20mansoni....pdf.txtc5ed74d8d35cc30571e6be5a4b767980MD53icict/460022023-03-15 14:34:29.6oai:www.arca.fiocruz.br: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ório 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| dc.title.pt_BR.fl_str_mv |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| title |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| spellingShingle |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function Perally, Samirah Veneno Schistosoma mansoni Tegumento Venom allergen-like Schistosoma mansoni Tegument |
| title_short |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| title_full |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| title_fullStr |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| title_full_unstemmed |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| title_sort |
Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function |
| author |
Perally, Samirah |
| author_facet |
Perally, Samirah Geyer, Kathrin K. Farani, Priscila S.G. Chalmers, Iain W. Fuentes, Narcis Fernandez Maskell, Daniel R. Hulme, Benjamin J. Thomas, Josephine Forde Phillips, Dylan Farias, Leonardo P. Collins, James J. Hoffmann, Karl F. |
| author_role |
author |
| author2 |
Geyer, Kathrin K. Farani, Priscila S.G. Chalmers, Iain W. Fuentes, Narcis Fernandez Maskell, Daniel R. Hulme, Benjamin J. Thomas, Josephine Forde Phillips, Dylan Farias, Leonardo P. Collins, James J. Hoffmann, Karl F. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Perally, Samirah Geyer, Kathrin K. Farani, Priscila S.G. Chalmers, Iain W. Fuentes, Narcis Fernandez Maskell, Daniel R. Hulme, Benjamin J. Thomas, Josephine Forde Phillips, Dylan Farias, Leonardo P. Collins, James J. Hoffmann, Karl F. |
| dc.subject.other.pt_BR.fl_str_mv |
Veneno Schistosoma mansoni Tegumento |
| topic |
Veneno Schistosoma mansoni Tegumento Venom allergen-like Schistosoma mansoni Tegument |
| dc.subject.en.pt_BR.fl_str_mv |
Venom allergen-like Schistosoma mansoni Tegument |
| description |
KFH (Wellcome Trust, UK, WT084273/Z/07/Z) and JJC (National Institutes of Health (NIH), USA, 1R01AI121037). PSGF was supported by the Brazilian Government’s Science Without Borders scheme. |
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2020 |
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2020 |
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2021-02-09T12:22:46Z |
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2021-02-09T12:22:46Z |
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PERALLY, Samirah et al. Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function. International Journal for Parasitology, 2020. |
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https://www.arca.fiocruz.br/handle/icict/46002 |
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0020-7519 |
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10.1016/j.ijpara.2020.09.004 |
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PERALLY, Samirah et al. Schistosoma mansoni venom allergen-like protein 6 (SmVAL6) maintains tegumental barrier function. International Journal for Parasitology, 2020. 0020-7519 10.1016/j.ijpara.2020.09.004 |
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