Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)

Detalhes bibliográficos
Autor(a) principal: Faria, Clarissa Perez
Data de Publicação: 2016
Outros Autores: Zanini, Graziela Maria, Dias, Gisele Silva, Silva, Sidnei da, Sousa, Maria do Céu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32664
Resumo: University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / Faculty of Pharmacy. University of Coimbra. Coimbra, Portugal / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Faria, Clarissa PerezZanini, Graziela MariaDias, Gisele SilvaSilva, Sidnei daSousa, Maria do Céu2019-04-22T14:51:49Z2019-04-22T14:51:49Z2016FARIA, Clarissa Perez et al. Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil). Plos One, p. 1-14, Aug. 12, 20161932-6203https://www.arca.fiocruz.br/handle/icict/3266410.1371/journal.pone.01607621932-6203engPublic Library of ScienceMolecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / Faculty of Pharmacy. University of Coimbra. Coimbra, Portugal / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / Faculty of Pharmacy. University of Coimbra. Coimbra, Portugal.Background: Despite the high prevalence of giardiasis, the genetic characterization of Giardia lamblia has been poorly documented in Brazil and molecular epidemiology research has only been conducted in the last few years. The aim of this study was to determine the prevalence of different G. lamblia assemblages and detect mixed infections among patients with giardiasis. Methods and Principal Findings: The cross-section survey was conducted among patients attending the FIOCRUZ in Rio de Janeiro. In order to discriminate the genetic assemblages/sub-assemblages, G. lamblia isolates were characterized by PCR-RFLP and qPCR using four loci genes (bg, gdh, tpi and orfC4). Of the 65 positive samples, 41 (63.1%) were successfully amplified by nested-PCR of bg and gdh genes. Among them, 16 were typed as sub-assemblage AII, 7 as BIII, 4 as BIV and 8 as a mixture of BIII and BIV. After the analysis by qPCR assay, a total of 55 (84.6%) samples were amplified using at least one locus: bg gene was amplified in 38 (58.5%) samples, gdh in 41 (63.1%), tpi in 39 (60%), and orfC4 in 39 (60%). Multilocus genotyping results showed that 29 (52.7%) samples belonged to Assemblage A and 26 (47.3%) samples belonged to Assemblage B. In 2011 and 2012, 20 (74.1%) samples belonged to Assemblage A and 7 (25.9%) belonged to Assemblage B. In subsequent years (2013–2015) there was a predominance of Assemblage B, 19 (67.9%) versus 9 (32.1%) Assemblage A. Conclusions: This is the first time that Assemblage B of G. lamblia was reported in human clinical samples from Rio de Janeiro (Brazil) and is the first report about genetic characterization using four genes. The qPCR assemblage-specific showed no mixed infections by Assemblages A and B. A switch in genetic profile over the years was observed, firstly predominance of Assemblage A and lastly of Assemblage B.Giardia lambliaMolecular characterizationRio de JaneiroBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32664/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Faria_Clarissa_etal_INI_2016.pdfve_Faria_Clarissa_etal_INI_2016.pdfapplication/pdf263256https://www.arca.fiocruz.br/bitstream/icict/32664/2/ve_Faria_Clarissa_etal_INI_2016.pdf8c3ae69794b40686841a30f9717c1bd0MD52TEXTve_Faria_Clarissa_etal_INI_2016.pdf.txtve_Faria_Clarissa_etal_INI_2016.pdf.txtExtracted texttext/plain51382https://www.arca.fiocruz.br/bitstream/icict/32664/3/ve_Faria_Clarissa_etal_INI_2016.pdf.txt773a210a3aa8ed0b82c4212ea7e98220MD53icict/326642019-04-23 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dc.title.pt_BR.fl_str_mv Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
title Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
spellingShingle Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
Faria, Clarissa Perez
Giardia lamblia
Molecular characterization
Rio de Janeiro
Brazil
title_short Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
title_full Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
title_fullStr Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
title_full_unstemmed Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
title_sort Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil)
author Faria, Clarissa Perez
author_facet Faria, Clarissa Perez
Zanini, Graziela Maria
Dias, Gisele Silva
Silva, Sidnei da
Sousa, Maria do Céu
author_role author
author2 Zanini, Graziela Maria
Dias, Gisele Silva
Silva, Sidnei da
Sousa, Maria do Céu
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Faria, Clarissa Perez
Zanini, Graziela Maria
Dias, Gisele Silva
Silva, Sidnei da
Sousa, Maria do Céu
dc.subject.en.pt_BR.fl_str_mv Giardia lamblia
Molecular characterization
Rio de Janeiro
Brazil
topic Giardia lamblia
Molecular characterization
Rio de Janeiro
Brazil
description University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / Faculty of Pharmacy. University of Coimbra. Coimbra, Portugal / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-04-22T14:51:49Z
dc.date.available.fl_str_mv 2019-04-22T14:51:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Clarissa Perez et al. Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil). Plos One, p. 1-14, Aug. 12, 2016
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32664
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0160762
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv FARIA, Clarissa Perez et al. Molecular characterization of Giardia lamblia: first report of assemblage B in human isolates from Rio de Janeiro (Brazil). Plos One, p. 1-14, Aug. 12, 2016
1932-6203
10.1371/journal.pone.0160762
url https://www.arca.fiocruz.br/handle/icict/32664
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32664/1/license.txt
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