Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19432 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
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Martins, Patricia PaisLampe, ElisabethLewis-Ximenez, Lia LauraSouza, Paulo Sergio Fonseca deFernandes, Carlos AugustoVillar, Livia Melo2017-06-20T13:35:23Z2017-06-20T13:35:23Z2013MARTINS, Patricia Pais et al. Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection. Clinical Laboratory, v. 59, p. 1031-1039, 2013.1433-6510https://www.arca.fiocruz.br/handle/icict/1943210.7754/Clin.Lab.2013.120903engClinical Laboratory PublicationsHepatite CDiagnóstico molecularPerformanceHepatitis CMolecular diagnosisPerformancePerformance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública Noel Nutels. Divisão de Hepatites. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Background: Molecular methods are essential to define hepatitis C virus (HCV) infection. This study was conducted to evaluate the performance of molecular qualitative and quantitative methods for HCV RNA among chronic patients and individuals during the course of HCV infection. Methods: Single serum samples were obtained from 82 HCV infected individuals where six of them donated serial serum samples (n = 52) during the course of HCV infection. Qualitative (in-house RT-nested PCR and COBAS® AMPLICOR HCV Test v2.0 and TMA) and quantitative (COBAS® AMPLICOR HCV Monitor Test v2.0 and bDNA) techniques were employed. Results: TMA presented the highest rate (87.8%) of HCV detection among qualitative tests and it was the most sensitive for HCV RNA detection during the early and late phases of HCV infection. HCV RNA was quantified among 56 samples and significant correlation was observed between the two assays (r 0.92; p < 0.0001). Conclusions: It is concluded that both quantitative methods can be used among chronic and acute HCV cases, but TMA was the most efficient for HCV qualitative detection among chronic cases and in the early and late phases of HCV infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19432/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALlivia_villar3_etal_IOC_2013.pdflivia_villar3_etal_IOC_2013.pdfapplication/pdf247705https://www.arca.fiocruz.br/bitstream/icict/19432/2/livia_villar3_etal_IOC_2013.pdfc0dd3241735d56d48d746c4bcbcde48dMD52TEXTlivia_villar3_etal_IOC_2013.pdf.txtlivia_villar3_etal_IOC_2013.pdf.txtExtracted texttext/plain41463https://www.arca.fiocruz.br/bitstream/icict/19432/3/livia_villar3_etal_IOC_2013.pdf.txtbb84766ae61dffb2473b2a389ff26456MD53icict/194322021-02-16 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| dc.title.pt_BR.fl_str_mv |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| title |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| spellingShingle |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection Martins, Patricia Pais Hepatite C Diagnóstico molecular Performance Hepatitis C Molecular diagnosis Performance |
| title_short |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| title_full |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| title_fullStr |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| title_full_unstemmed |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| title_sort |
Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection |
| author |
Martins, Patricia Pais |
| author_facet |
Martins, Patricia Pais Lampe, Elisabeth Lewis-Ximenez, Lia Laura Souza, Paulo Sergio Fonseca de Fernandes, Carlos Augusto Villar, Livia Melo |
| author_role |
author |
| author2 |
Lampe, Elisabeth Lewis-Ximenez, Lia Laura Souza, Paulo Sergio Fonseca de Fernandes, Carlos Augusto Villar, Livia Melo |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Martins, Patricia Pais Lampe, Elisabeth Lewis-Ximenez, Lia Laura Souza, Paulo Sergio Fonseca de Fernandes, Carlos Augusto Villar, Livia Melo |
| dc.subject.other.pt_BR.fl_str_mv |
Hepatite C Diagnóstico molecular Performance |
| topic |
Hepatite C Diagnóstico molecular Performance Hepatitis C Molecular diagnosis Performance |
| dc.subject.en.pt_BR.fl_str_mv |
Hepatitis C Molecular diagnosis Performance |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013 |
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2017-06-20T13:35:23Z |
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2017-06-20T13:35:23Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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MARTINS, Patricia Pais et al. Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection. Clinical Laboratory, v. 59, p. 1031-1039, 2013. |
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https://www.arca.fiocruz.br/handle/icict/19432 |
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1433-6510 |
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10.7754/Clin.Lab.2013.120903 |
| identifier_str_mv |
MARTINS, Patricia Pais et al. Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection. Clinical Laboratory, v. 59, p. 1031-1039, 2013. 1433-6510 10.7754/Clin.Lab.2013.120903 |
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Clinical Laboratory Publications |
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Clinical Laboratory Publications |
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