A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7724 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil. |
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Fraga, Deborah Bittencourt MothéSilva, Edimilson Domingos daPacheco, Luciano VasconcellosBorja, Lairton SouzaOliveira, Isaac Queiroz deVital, Wendel CouraMonteiro, Glória ReginaOliveira, Geraldo Gileno de SáJerônimo, Selma Maria BezerraReis, Alexandre BarbosaVeras, Patrícia Sampaio Tavares2014-05-29T17:44:17Z2014-05-29T17:44:17Z2014FRAGA, D. B. M. et al. A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. Parasites & Vectors, v. 7, p. 136-140, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/772410.1186/1756-3305-7-136engBioMed CentralA multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Diagnóstica. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilInstituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil /Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil / Universidade Federal de Ouro Preto. Laboratório de Imunopatologia. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilInstituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil /Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Natal, RGN, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, BrasilInstituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil / Universidade Federal do Rio Grande do Norte. Departamento de Bioquímica. Centro de Biociências. Natal, RGN, BrasilInstituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil / Universidade Federal de Ouro Preto. Laboratório de Imunopatologia. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, BrasilBACKGROUND: Visceral leishmaniasis (VL) is a serious public health challenge in Brazil and dogs are considered to be the main urban reservoir of the causative agent. The culling of animals to control VL in some countries makes the accurate diagnosis of canine VL (CVL) essential. Recombinant antigens rLci1A and rLci2B were selected from a cDNA library of Leishmania infantum amastigotes due to their strong potential as candidates in diagnostic testing for CVL. The present multicentric study aimed to evaluate the sensitivity of a prototype test using these antigens (DPP rLci1A/rLci2B) against 154 sera obtained from symptomatic dogs within three endemic areas of VL in Brazil. The specificity was evaluated using 40 serum samples from negative dogs and dogs infected with other pathogens. Sensitivity and specificity rates of DPP rLci1A/rLci2B prototype were compared to rates from other diagnostic tests currently in use by the Brazilian Ministry of Health, including DPPLVC, EIELVC. FINDINGS: DPP rLci1A/rLci2B prototype offered similar performance to that offered by DPPLVC rapid test, as follows: sensitivity of 87% (CI 81-91) and 88% (CI 82-93) and specificity of 100% (CI 91-100) and 97% (CI 87-100), respectively for DPP rLci1A/rLci2B and DPPLVC. When results of these two tests were considered concomitantly, sensitivity increased to 93.5% (CI 89-96). CONCLUSIONS: The recombinant antigens rLci1A and rLci2B represent promising candidates for use in a multi-antigen rapid test for CVL. The inclusion of novel antigens to the DPP rLci1A/rLci2B prototype model could offer additionally enhanced sensitivity to detect animals infected by L. infantum.Canine visceral leishmaniasisSerological diagnosisRecombinant antigenRapid testMulticentric studyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFraga DBM A multicentric....pdfFraga DBM A multicentric....pdfapplication/pdf220261https://www.arca.fiocruz.br/bitstream/icict/7724/1/Fraga%20DBM%20A%20multicentric....pdfb968cd768147d9c019d8fa251326b8a6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7724/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFraga DBM A multicentric....pdf.txtFraga DBM A multicentric....pdf.txtExtracted texttext/plain22840https://www.arca.fiocruz.br/bitstream/icict/7724/5/Fraga%20DBM%20A%20multicentric....pdf.txt21edbb0280bfdd5d3ac7c660f1d8915dMD55THUMBNAILFraga DBM A multicentric....pdf.jpgFraga DBM A multicentric....pdf.jpgGenerated Thumbnailimage/jpeg2405https://www.arca.fiocruz.br/bitstream/icict/7724/4/Fraga%20DBM%20A%20multicentric....pdf.jpg8ac768f61443522d7574eccd894d8a8fMD54icict/77242023-03-15 14:33:21.853oai:www.arca.fiocruz.br:icict/7724TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| title |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| spellingShingle |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. Fraga, Deborah Bittencourt Mothé Canine visceral leishmaniasis Serological diagnosis Recombinant antigen Rapid test Multicentric study |
| title_short |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| title_full |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| title_fullStr |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| title_full_unstemmed |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| title_sort |
A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. |
| author |
Fraga, Deborah Bittencourt Mothé |
| author_facet |
Fraga, Deborah Bittencourt Mothé Silva, Edimilson Domingos da Pacheco, Luciano Vasconcellos Borja, Lairton Souza Oliveira, Isaac Queiroz de Vital, Wendel Coura Monteiro, Glória Regina Oliveira, Geraldo Gileno de Sá Jerônimo, Selma Maria Bezerra Reis, Alexandre Barbosa Veras, Patrícia Sampaio Tavares |
| author_role |
author |
| author2 |
Silva, Edimilson Domingos da Pacheco, Luciano Vasconcellos Borja, Lairton Souza Oliveira, Isaac Queiroz de Vital, Wendel Coura Monteiro, Glória Regina Oliveira, Geraldo Gileno de Sá Jerônimo, Selma Maria Bezerra Reis, Alexandre Barbosa Veras, Patrícia Sampaio Tavares |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fraga, Deborah Bittencourt Mothé Silva, Edimilson Domingos da Pacheco, Luciano Vasconcellos Borja, Lairton Souza Oliveira, Isaac Queiroz de Vital, Wendel Coura Monteiro, Glória Regina Oliveira, Geraldo Gileno de Sá Jerônimo, Selma Maria Bezerra Reis, Alexandre Barbosa Veras, Patrícia Sampaio Tavares |
| dc.subject.en.pt_BR.fl_str_mv |
Canine visceral leishmaniasis Serological diagnosis Recombinant antigen Rapid test Multicentric study |
| topic |
Canine visceral leishmaniasis Serological diagnosis Recombinant antigen Rapid test Multicentric study |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais, INCT-DT. Salvador, BA, Brasil. |
| publishDate |
2014 |
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2014-05-29T17:44:17Z |
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2014-05-29T17:44:17Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
FRAGA, D. B. M. et al. A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. Parasites & Vectors, v. 7, p. 136-140, 2014. |
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https://www.arca.fiocruz.br/handle/icict/7724 |
| dc.identifier.issn.none.fl_str_mv |
1756-3305 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/1756-3305-7-136 |
| identifier_str_mv |
FRAGA, D. B. M. et al. A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. Parasites & Vectors, v. 7, p. 136-140, 2014. 1756-3305 10.1186/1756-3305-7-136 |
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BioMed Central |
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BioMed Central |
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