In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30910 |
Resumo: | Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. |
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Verza, MirelaMaschmann, Raquel de AbreuSilva, Márcia Susana NunesCosta, Elis Regina DallaRibeiro, Marta OsórioRosso, FrancieleSuffys, Philip NoelTortoli, EnricoMarcelli, FiorellaZaha, ArnaldoRossetti, Maria Lucia Rosa2019-01-08T11:50:54Z2019-01-08T11:50:54Z2009VERZA, Mirela; et al. In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis. Mem Inst Oswaldo Cruz, v.104, n.5, p.710-714, Aug. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/309101678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzTuberculoseResistência a medicamentosensaio de hibridização reversaIsoniazidadrug resistanceisoniazidkatG315reverse hybridization assaytuberculosisIsoniazidaIn house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Canoas, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Centro Regionale di Riferimento per i Micobatteri. Ospedale di Careggi, Firenze, Italy.Centro Regionale di Riferimento per i Micobatteri. Ospedale di Careggi, Firenze, Italy.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Canoas, RS, Brasil.Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30910/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALphilipnoel2_suffys_etal_IOC_2009.pdfphilipnoel2_suffys_etal_IOC_2009.pdfapplication/pdf392983https://www.arca.fiocruz.br/bitstream/icict/30910/2/philipnoel2_suffys_etal_IOC_2009.pdf303700818916c68d04775622b1473780MD52TEXTphilipnoel2_suffys_etal_IOC_2009.pdf.txtphilipnoel2_suffys_etal_IOC_2009.pdf.txtExtracted texttext/plain29344https://www.arca.fiocruz.br/bitstream/icict/30910/3/philipnoel2_suffys_etal_IOC_2009.pdf.txtd43078b4920c3ebd0dcb3fd2f68d8df5MD53icict/309102019-01-09 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| dc.title.pt_BR.fl_str_mv |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| title |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| spellingShingle |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis Verza, Mirela Tuberculose Resistência a medicamentos ensaio de hibridização reversa Isoniazida drug resistance isoniazid katG315 reverse hybridization assay tuberculosis Isoniazida |
| title_short |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| title_full |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| title_fullStr |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| title_full_unstemmed |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| title_sort |
In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis |
| author |
Verza, Mirela |
| author_facet |
Verza, Mirela Maschmann, Raquel de Abreu Silva, Márcia Susana Nunes Costa, Elis Regina Dalla Ribeiro, Marta Osório Rosso, Franciele Suffys, Philip Noel Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| author_role |
author |
| author2 |
Maschmann, Raquel de Abreu Silva, Márcia Susana Nunes Costa, Elis Regina Dalla Ribeiro, Marta Osório Rosso, Franciele Suffys, Philip Noel Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Verza, Mirela Maschmann, Raquel de Abreu Silva, Márcia Susana Nunes Costa, Elis Regina Dalla Ribeiro, Marta Osório Rosso, Franciele Suffys, Philip Noel Tortoli, Enrico Marcelli, Fiorella Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| dc.subject.other.pt_BR.fl_str_mv |
Tuberculose Resistência a medicamentos ensaio de hibridização reversa Isoniazida |
| topic |
Tuberculose Resistência a medicamentos ensaio de hibridização reversa Isoniazida drug resistance isoniazid katG315 reverse hybridization assay tuberculosis Isoniazida |
| dc.subject.en.pt_BR.fl_str_mv |
drug resistance isoniazid katG315 reverse hybridization assay tuberculosis |
| dc.subject.decs.pt_BR.fl_str_mv |
Isoniazida |
| description |
Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. |
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2009 |
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2009 |
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2019-01-08T11:50:54Z |
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2019-01-08T11:50:54Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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VERZA, Mirela; et al. In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis. Mem Inst Oswaldo Cruz, v.104, n.5, p.710-714, Aug. 2009. |
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https://www.arca.fiocruz.br/handle/icict/30910 |
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0074-0276 |
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1678-8060 |
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VERZA, Mirela; et al. In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis. Mem Inst Oswaldo Cruz, v.104, n.5, p.710-714, Aug. 2009. 0074-0276 1678-8060 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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