Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9918 |
Resumo: | University of California. Fielding School of Public Health. Department of Epidemiology. Los Angeles, USA. |
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Bristow, ClaireAdachi, KristinaNielsen-Saines, KarinAnk, BonniePilotto, José HenriqueJoao, Esau C.Klausner, Jeffrey D.Veloso, Valdiléa G.Morgado, Mariza G.Watts, D. Heather2015-04-08T14:09:57Z2015-04-08T14:09:57Z2014BRISTOW, Claire et al. Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens. Journal of Microbiology & Experimentation, v. 1, n. 4, p. 1-5, 2014.2373-437Xhttps://www.arca.fiocruz.br/handle/icict/991810.15406/jmen.2014.01.00026engMedCraveDoenças Sexualmente TransmissíveisGonorreiaInfecções por ChlamydiaSample adequacy controlChlamydiaGonorrheaDiagnosisInflammationChlamydiaGonorreiaSexually Transmitted DiseasesCharacteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of California. Fielding School of Public Health. Department of Epidemiology. Los Angeles, USA.University of California. David Geffen UCLA School of Medicine. USA.University of California. David Geffen UCLA School of Medicine. USA.University of California. David Geffen UCLA School of Medicine. USA.Hospital Geral de Nova Iguaçu. Nova Iguaçu, RJ, Brazil.Hospital Federal dos Servidores do Estado. Rio de Janeiro, RJ, Brazil.University of California. David Geffen UCLA School of Medicine. USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clinica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.National Institutes of Health. Eunice Kennedy Shriver National Institute of Child Health and Human Development. USA.Background: The Xpert® CT/NG (Cepheid Sunnyvale, CA) is a rapid, fully automated real-time polymerase chain reaction test that simultaneously detects Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG). It has high sensitivity and specificity, but also includes a Specimen Adequacy Control (SAC). SAC controls for false negative results by confirming adequate patient sample and appropriate testing conditions. SAC is quantified by its cycle threshold (Ct), the number of cycles required to detect the presence of a single copy human gene. A lower SAC indicates an earlier Ct and more human cellular material detected. Our objectives were to describe the frequency and distribution of SAC Ct values and observe any correlations with detected infections. Methods: Urine samples from 1382 HIV-1-infected pregnant women, collected at the time of labor/delivery underwent Xpert® CT/NG testing. Mean SAC Ct values and standard deviation (SD) were calculated. Student’s t-test was used to compare mean SAC Ct values to a reference of urine samples negative for CT and NG. Results: The urine CT positivity was 17.9% (248/1382) and NG, 4.6% (63/1382). The mean SAC Ct value in urine from women without CT or NG was 28.09 (SD: 4.12) and higher than the mean SAC Ct value for CT positive specimens (27.29, SD: 3.84(P=.0054)), NG positive specimens (26.23, SD: 3.09(P<.0001)), specimens positive for both CT and NG (26.41, SD: 3.01(P=.0027)). Conclusion: Lower SAC Ct values were significantly associated with chlamydial and gonococcal infections. Further studies should be conducted to determine the utility of SAC Ct values for identifying the presence of increased human cellular material and infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/9918/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmariza_morgadoetal_IOC_2014.pdfapplication/pdf1843908https://www.arca.fiocruz.br/bitstream/icict/9918/2/mariza_morgadoetal_IOC_2014.pdfbfc9e7b4bbe85317564c2073d3a1d8a7MD52TEXTmariza_morgadoetal_IOC_2014.pdf.txtmariza_morgadoetal_IOC_2014.pdf.txtExtracted texttext/plain23611https://www.arca.fiocruz.br/bitstream/icict/9918/3/mariza_morgadoetal_IOC_2014.pdf.txt03c36a9fcaa9c6bbde52812c2264f18eMD53icict/99182023-03-29 22:49:18.244oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-30T01:49:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| title |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| spellingShingle |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens Bristow, Claire Doenças Sexualmente Transmissíveis Gonorreia Infecções por Chlamydia Sample adequacy control Chlamydia Gonorrhea Diagnosis Inflammation Chlamydia Gonorreia Sexually Transmitted Diseases |
| title_short |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| title_full |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| title_fullStr |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| title_full_unstemmed |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| title_sort |
Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens |
| author |
Bristow, Claire |
| author_facet |
Bristow, Claire Adachi, Kristina Nielsen-Saines, Karin Ank, Bonnie Pilotto, José Henrique Joao, Esau C. Klausner, Jeffrey D. Veloso, Valdiléa G. Morgado, Mariza G. Watts, D. Heather |
| author_role |
author |
| author2 |
Adachi, Kristina Nielsen-Saines, Karin Ank, Bonnie Pilotto, José Henrique Joao, Esau C. Klausner, Jeffrey D. Veloso, Valdiléa G. Morgado, Mariza G. Watts, D. Heather |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bristow, Claire Adachi, Kristina Nielsen-Saines, Karin Ank, Bonnie Pilotto, José Henrique Joao, Esau C. Klausner, Jeffrey D. Veloso, Valdiléa G. Morgado, Mariza G. Watts, D. Heather |
| dc.subject.other.pt_BR.fl_str_mv |
Doenças Sexualmente Transmissíveis Gonorreia Infecções por Chlamydia |
| topic |
Doenças Sexualmente Transmissíveis Gonorreia Infecções por Chlamydia Sample adequacy control Chlamydia Gonorrhea Diagnosis Inflammation Chlamydia Gonorreia Sexually Transmitted Diseases |
| dc.subject.en.pt_BR.fl_str_mv |
Sample adequacy control Chlamydia Gonorrhea Diagnosis Inflammation |
| dc.subject.decs.pt_BR.fl_str_mv |
Chlamydia Gonorreia Sexually Transmitted Diseases |
| description |
University of California. Fielding School of Public Health. Department of Epidemiology. Los Angeles, USA. |
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2014 |
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2014 |
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2015-04-08T14:09:57Z |
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2015-04-08T14:09:57Z |
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BRISTOW, Claire et al. Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens. Journal of Microbiology & Experimentation, v. 1, n. 4, p. 1-5, 2014. |
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https://www.arca.fiocruz.br/handle/icict/9918 |
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2373-437X |
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10.15406/jmen.2014.01.00026 |
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BRISTOW, Claire et al. Characteristics of the Sample Adequacy Control (SAC) in the Cepheid Xpert® CCT/NG Assay in Female Urine Specimens. Journal of Microbiology & Experimentation, v. 1, n. 4, p. 1-5, 2014. 2373-437X 10.15406/jmen.2014.01.00026 |
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