HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.

Detalhes bibliográficos
Autor(a) principal: Rodríguez Lay, Licel de los Angeles
Data de Publicação: 2012
Outros Autores: Villalba, Maria Caridad Montalvo, Corredora, Marité Bello, Frómeta, Susel Sariego, Hernández, Jeny Marante, Carrera, Santiago Dueñas, Wonga, Meilin Sánchez, Samada, Marcia, Núñez, Milay Bello, Alonso, Lidunka Valdes, Silva Filho, Hermes Pedreira da, Hübschenf, Judith M., Reis, Mitermayer Galvão dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7493
Resumo: Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
id CRUZ_3c914fe52b1701af7478c7a95939c31d
oai_identifier_str oai:www.arca.fiocruz.br:icict/7493
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Rodríguez Lay, Licel de los AngelesVillalba, Maria Caridad MontalvoCorredora, Marité BelloFrómeta, Susel SariegoHernández, Jeny MaranteCarrera, Santiago DueñasWonga, Meilin SánchezSamada, MarciaNúñez, Milay BelloAlonso, Lidunka ValdesSilva Filho, Hermes Pedreira daHübschenf, Judith M.Reis, Mitermayer Galvão dos2014-04-03T19:02:16Z2014-04-03T19:02:16Z2012RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012.1878-350310.1016/j.trstmh.2012.08.001https://www.arca.fiocruz.br/handle/icict/7493engElsevier LtdHCV genotype determination in monoinfected and HIV co-infected patients in Cuba.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCenter for Genetic Engineering and Biotechnology (CIGB). Havana, CubaInstitute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, CubaInstitute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, CubaInstitute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, CubaCenter for Genetic Engineering and Biotechnology (CIGB). Havana, CubaCenter for Genetic Engineering and Biotechnology (CIGB). Havana, CubaCenter for Genetic Engineering and Biotechnology (CIGB). Havana, CubaCentro de Investigaciones Médico-Quirúrgicas. CIMEQ. Havana, CubaIvan Portuondo General Hospital. San Antonio de los Baños. Havana, CubaInstitute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, CubaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilInstitute of Immunology. CRP-Santé/Laboratoire National de Santé. Luxembourg, LuxembourgFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilWith the aim to characterize the HCV genotype distribution in Cuba, sera were collected from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination of reverse transcription-PCR using genotype-specific primers, restriction fragment length polymorphism and sequencing was used to determine the genotype of 84 samples. Seventy-nine (94%) showed single infections (10 [12%] were genotype 1a and 69 [82%] genotype 1b) and 5 (6%) samples corresponded to mixed infections (2 [2%] with genotypes 1a/3a and 1 sample [1%] each with 1b/3a, 1b/4a and 1a/1b/3a). HCV/HIV co-infected subjects had a higher frequency of mixed infections (p=0.08), infection with genotype 3a (p=0.18) and for the first time genotype 4a was found. There was no association of any demographic characteristics with any specific genotype although HCV/HIV co-infected patients showed a tendency to have mixed genotypes in those older than 45 years of age (p=0.11). Phylogenetic analysis showed that HCV isolates clustered with subtypes 1b (n=15, maximal genetic distance 2.51%) and 1a (n=2, maximal genetic distance 0.35%). This report presents the prevalence of HCV genotypes in monoinfected and HIV co-infected patients, mixed HCV infections in HCV/HIV co-infected men who have sex with men with high-risk sexual practices and for the first time identifies that the uncommon genotype 4a can be present in a patient co-infected with HIV.HCVHIVHCV/HIV co-infectionMolecular epidemiologyPhylogenetic analysisRisk groupsRNA Viral/genéticaCoinfecção/virologiaInfecções por HIV/virologiaHepacivirus/genéticaHepatite C/virologiaAdolescenteAdultoIdosoCriançaCoinfecção/epidemiologiaCuba/epidemiologiaPrimers do DNA/genéticaFemininoGenótipoInfecções por HIV/epidemiologiaHepacivirus/classificaçãoHepatite C/diagnósticoHepatite C/epidemiologiaHomossexualidade MasculinaHumanosMasculinoMeia-IdadeEpidemiologia Molecularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRodriguez Lay LLA HCV genotype determination....pdfRodriguez Lay LLA HCV genotype determination....pdfapplication/pdf269278https://www.arca.fiocruz.br/bitstream/icict/7493/1/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdfa65eb902043bdc0d580017ed861af272MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7493/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTRodriguez Lay LLA HCV genotype determination....pdf.txtRodriguez Lay LLA HCV genotype determination....pdf.txtExtracted texttext/plain38459https://www.arca.fiocruz.br/bitstream/icict/7493/5/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdf.txtf8c2eb3b61f2bef83f888b180569c8d8MD55THUMBNAILRodriguez Lay LLA HCV genotype determination....pdf.jpgRodriguez Lay LLA HCV genotype determination....pdf.jpgGenerated Thumbnailimage/jpeg2230https://www.arca.fiocruz.br/bitstream/icict/7493/4/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdf.jpgb5ff21948b4d25e94f64a1b42a77adc6MD54icict/74932023-03-15 14:33:11.793oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
title HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
spellingShingle HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
Rodríguez Lay, Licel de los Angeles
HCV
HIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
Coinfecção/virologia
Infecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
title_short HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
title_full HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
title_fullStr HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
title_full_unstemmed HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
title_sort HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
author Rodríguez Lay, Licel de los Angeles
author_facet Rodríguez Lay, Licel de los Angeles
Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
author_role author
author2 Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodríguez Lay, Licel de los Angeles
Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
dc.subject.en.pt_BR.fl_str_mv HCV
HIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
topic HCV
HIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
Coinfecção/virologia
Infecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
dc.subject.decs.pt_BR.fl_str_mv Coinfecção/virologia
Infecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
description Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-04-03T19:02:16Z
dc.date.available.fl_str_mv 2014-04-03T19:02:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7493
dc.identifier.issn.none.fl_str_mv 1878-3503
10.1016/j.trstmh.2012.08.001
identifier_str_mv RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012.
1878-3503
10.1016/j.trstmh.2012.08.001
url https://www.arca.fiocruz.br/handle/icict/7493
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7493/1/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdf
https://www.arca.fiocruz.br/bitstream/icict/7493/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7493/5/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7493/4/Rodriguez%20Lay%20LLA%20HCV%20genotype%20determination....pdf.jpg
bitstream.checksum.fl_str_mv a65eb902043bdc0d580017ed861af272
7d48279ffeed55da8dfe2f8e81f3b81f
f8c2eb3b61f2bef83f888b180569c8d8
b5ff21948b4d25e94f64a1b42a77adc6
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324686160920576

Registros relacionados