Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57913 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. |
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Temerozo, Jairo R.Ferreira, Pedro L. C.Lacerda, Leandra LinharesVieira, Rhaíssa C.Alves, Bruno CisterGobbo, LiviaAlves, Marcelo RibeiroMenna Barreto, Rubem F. S.Bou-Habib, Dumith Chequer2023-04-23T21:20:04Z2023-04-23T21:20:04Z2023TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023.1098-5514https://www.arca.fiocruz.br/handle/icict/5791310.1128/jvi.01752-22engAmerican Society for MicrobiologyHIV-1IL-27BST-2/TetherinPBMCsHIV-1IL-27BST-2/TetherinPBMCsInterleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil .Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório da Imunobiologia da Leishmaniose. Rio de Janeiro, RJ, Brasil / Instituto nacional de Ciência e tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, Centro de Pesquisa Clínica HIV e AIDS. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciênciae Tecnologia em Neuroimunomodulação. Riode Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Interleukin-27 (IL-27) is able to inhibit HIV-1 replication in peripheral blood mononuclear cells (PBMCs), macrophages, and dendritic cells. Here, we identify that IL-27 can produce opposing effects on HIV-1 replication in PBMCs and that the HIV-1 restriction factor BST-2/Tetherin is involved in both inhibitory and enhancing effects on HIV-1 infection induced by IL-27. IL-27 inhibited HIV-1 replication when added to cells 2 h after infection, promoting the prototypical BST-2/Tetherin-induced virion accumulation at the cell membrane of HIV-1-infected PBMCs. BST-2/Tetherin gene expression was significantly upregulated in the IL-27-treated PBMCs, with a simultaneous increase in the number of BST- 2/Tetherin1 cells. The silencing of BST-2/Tetherin diminished the anti-HIV-1 effect of IL-27. In contrast, IL-27 increased HIV-1 production when added to infected cells 4 days after infection. This enhancing effect was prevented by BST-2/Tetherin gene knockdown, which also permitted IL-27 to function again as an HIV-1 inhibitory factor. These contrasting roles of IL-27 were associated with the dynamic of viral production, since the IL-27-mediated enhancement of virus replication was prevented by antiretroviral treatment of infected cells, as well as by keeping cells under agitation to avoid cell-to-cell contact. Likewise, inhibition of CD11a, an integrin associated with HIV-1 cell-to-cell transmission, abrogated the IL-27 enhancement of HIV-1 production. Our findings illustrate the complexity of the HIV-1–host interactions and may impact the potential therapeutic use of IL-27 and other soluble mediators that induce BST-2/Tetherin expression for HIV-1 infection. IMPORTANCE Here, we describe new findings related to the ability of the cytokine IL-27 to regulate the growth of HIV-1 in CD41 T lymphocytes. IL-27 has long been considered a potent inhibitor of HIV-1 replication, a notion based on several reports showing that this cytokine controls HIV-1 infection in peripheral blood mononuclear cells (PBMCs), monocyte-derived macrophages, and dendritic cells. However, our present results are contrary to the current knowledge that IL-27 acts only as a powerful downregulator of HIV-1 replication. We observed that IL-27 can either prevent or enhance viral growth in PBMCs, an outcome dependent on when this cytokine is added to the infected cells. We detected that the increase of HIV-1 dissemination is due to enhanced cell-to-cell transmission with the involvement of the interferon-induced HIV-1 restriction factor BST-2/Tetherin and CD11a (LFA-1), an integrin that participates in formation of virological synapse.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57913/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJairoTemerozo_Dumithabib_etal_IOC_2023.pdfJairoTemerozo_Dumithabib_etal_IOC_2023.pdfapplication/pdf2730040https://www.arca.fiocruz.br/bitstream/icict/57913/2/JairoTemerozo_Dumithabib_etal_IOC_2023.pdf7d00fff64729d46f5019364fdb2e9845MD52icict/579132023-09-04 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| dc.title.en_US.fl_str_mv |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| title |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| spellingShingle |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin Temerozo, Jairo R. HIV-1 IL-27 BST-2/Tetherin PBMCs HIV-1 IL-27 BST-2/Tetherin PBMCs |
| title_short |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| title_full |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| title_fullStr |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| title_full_unstemmed |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| title_sort |
Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin |
| author |
Temerozo, Jairo R. |
| author_facet |
Temerozo, Jairo R. Ferreira, Pedro L. C. Lacerda, Leandra Linhares Vieira, Rhaíssa C. Alves, Bruno Cister Gobbo, Livia Alves, Marcelo Ribeiro Menna Barreto, Rubem F. S. Bou-Habib, Dumith Chequer |
| author_role |
author |
| author2 |
Ferreira, Pedro L. C. Lacerda, Leandra Linhares Vieira, Rhaíssa C. Alves, Bruno Cister Gobbo, Livia Alves, Marcelo Ribeiro Menna Barreto, Rubem F. S. Bou-Habib, Dumith Chequer |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Temerozo, Jairo R. Ferreira, Pedro L. C. Lacerda, Leandra Linhares Vieira, Rhaíssa C. Alves, Bruno Cister Gobbo, Livia Alves, Marcelo Ribeiro Menna Barreto, Rubem F. S. Bou-Habib, Dumith Chequer |
| dc.subject.other.en_US.fl_str_mv |
HIV-1 IL-27 BST-2/Tetherin PBMCs |
| topic |
HIV-1 IL-27 BST-2/Tetherin PBMCs HIV-1 IL-27 BST-2/Tetherin PBMCs |
| dc.subject.en.en_US.fl_str_mv |
HIV-1 IL-27 BST-2/Tetherin PBMCs |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. |
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2023 |
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2023-04-23T21:20:04Z |
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2023-04-23T21:20:04Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023. |
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https://www.arca.fiocruz.br/handle/icict/57913 |
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1098-5514 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/jvi.01752-22 |
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TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023. 1098-5514 10.1128/jvi.01752-22 |
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American Society for Microbiology |
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American Society for Microbiology |
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