Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin

Detalhes bibliográficos
Autor(a) principal: Temerozo, Jairo R.
Data de Publicação: 2023
Outros Autores: Ferreira, Pedro L. C., Lacerda, Leandra Linhares, Vieira, Rhaíssa C., Alves, Bruno Cister, Gobbo, Livia, Alves, Marcelo Ribeiro, Menna Barreto, Rubem F. S., Bou-Habib, Dumith Chequer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57913
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
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spelling Temerozo, Jairo R.Ferreira, Pedro L. C.Lacerda, Leandra LinharesVieira, Rhaíssa C.Alves, Bruno CisterGobbo, LiviaAlves, Marcelo RibeiroMenna Barreto, Rubem F. S.Bou-Habib, Dumith Chequer2023-04-23T21:20:04Z2023-04-23T21:20:04Z2023TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023.1098-5514https://www.arca.fiocruz.br/handle/icict/5791310.1128/jvi.01752-22engAmerican Society for MicrobiologyHIV-1IL-27BST-2/TetherinPBMCsHIV-1IL-27BST-2/TetherinPBMCsInterleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil .Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório da Imunobiologia da Leishmaniose. Rio de Janeiro, RJ, Brasil / Instituto nacional de Ciência e tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, Centro de Pesquisa Clínica HIV e AIDS. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciênciae Tecnologia em Neuroimunomodulação. Riode Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Interleukin-27 (IL-27) is able to inhibit HIV-1 replication in peripheral blood mononuclear cells (PBMCs), macrophages, and dendritic cells. Here, we identify that IL-27 can produce opposing effects on HIV-1 replication in PBMCs and that the HIV-1 restriction factor BST-2/Tetherin is involved in both inhibitory and enhancing effects on HIV-1 infection induced by IL-27. IL-27 inhibited HIV-1 replication when added to cells 2 h after infection, promoting the prototypical BST-2/Tetherin-induced virion accumulation at the cell membrane of HIV-1-infected PBMCs. BST-2/Tetherin gene expression was significantly upregulated in the IL-27-treated PBMCs, with a simultaneous increase in the number of BST- 2/Tetherin1 cells. The silencing of BST-2/Tetherin diminished the anti-HIV-1 effect of IL-27. In contrast, IL-27 increased HIV-1 production when added to infected cells 4 days after infection. This enhancing effect was prevented by BST-2/Tetherin gene knockdown, which also permitted IL-27 to function again as an HIV-1 inhibitory factor. These contrasting roles of IL-27 were associated with the dynamic of viral production, since the IL-27-mediated enhancement of virus replication was prevented by antiretroviral treatment of infected cells, as well as by keeping cells under agitation to avoid cell-to-cell contact. Likewise, inhibition of CD11a, an integrin associated with HIV-1 cell-to-cell transmission, abrogated the IL-27 enhancement of HIV-1 production. Our findings illustrate the complexity of the HIV-1–host interactions and may impact the potential therapeutic use of IL-27 and other soluble mediators that induce BST-2/Tetherin expression for HIV-1 infection. IMPORTANCE Here, we describe new findings related to the ability of the cytokine IL-27 to regulate the growth of HIV-1 in CD41 T lymphocytes. IL-27 has long been considered a potent inhibitor of HIV-1 replication, a notion based on several reports showing that this cytokine controls HIV-1 infection in peripheral blood mononuclear cells (PBMCs), monocyte-derived macrophages, and dendritic cells. However, our present results are contrary to the current knowledge that IL-27 acts only as a powerful downregulator of HIV-1 replication. We observed that IL-27 can either prevent or enhance viral growth in PBMCs, an outcome dependent on when this cytokine is added to the infected cells. We detected that the increase of HIV-1 dissemination is due to enhanced cell-to-cell transmission with the involvement of the interferon-induced HIV-1 restriction factor BST-2/Tetherin and CD11a (LFA-1), an integrin that participates in formation of virological synapse.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57913/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJairoTemerozo_Dumithabib_etal_IOC_2023.pdfJairoTemerozo_Dumithabib_etal_IOC_2023.pdfapplication/pdf2730040https://www.arca.fiocruz.br/bitstream/icict/57913/2/JairoTemerozo_Dumithabib_etal_IOC_2023.pdf7d00fff64729d46f5019364fdb2e9845MD52icict/579132023-09-04 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dc.title.en_US.fl_str_mv Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
title Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
spellingShingle Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
Temerozo, Jairo R.
HIV-1
IL-27
BST-2/Tetherin
PBMCs
HIV-1
IL-27
BST-2/Tetherin
PBMCs
title_short Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
title_full Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
title_fullStr Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
title_full_unstemmed Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
title_sort Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin
author Temerozo, Jairo R.
author_facet Temerozo, Jairo R.
Ferreira, Pedro L. C.
Lacerda, Leandra Linhares
Vieira, Rhaíssa C.
Alves, Bruno Cister
Gobbo, Livia
Alves, Marcelo Ribeiro
Menna Barreto, Rubem F. S.
Bou-Habib, Dumith Chequer
author_role author
author2 Ferreira, Pedro L. C.
Lacerda, Leandra Linhares
Vieira, Rhaíssa C.
Alves, Bruno Cister
Gobbo, Livia
Alves, Marcelo Ribeiro
Menna Barreto, Rubem F. S.
Bou-Habib, Dumith Chequer
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Temerozo, Jairo R.
Ferreira, Pedro L. C.
Lacerda, Leandra Linhares
Vieira, Rhaíssa C.
Alves, Bruno Cister
Gobbo, Livia
Alves, Marcelo Ribeiro
Menna Barreto, Rubem F. S.
Bou-Habib, Dumith Chequer
dc.subject.other.en_US.fl_str_mv HIV-1
IL-27
BST-2/Tetherin
PBMCs
topic HIV-1
IL-27
BST-2/Tetherin
PBMCs
HIV-1
IL-27
BST-2/Tetherin
PBMCs
dc.subject.en.en_US.fl_str_mv HIV-1
IL-27
BST-2/Tetherin
PBMCs
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-23T21:20:04Z
dc.date.available.fl_str_mv 2023-04-23T21:20:04Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57913
dc.identifier.issn.en_US.fl_str_mv 1098-5514
dc.identifier.doi.none.fl_str_mv 10.1128/jvi.01752-22
identifier_str_mv TEMEROZO, Jairo R. et al. Interleukin-27 Promotes Divergent Effects on HIV-1 Infection in Peripheral Blood Mononuclear Cells through BST-2/Tetherin. Journal of Virology, v.97, n. 1, p. 1-14, Jan. 2023.
1098-5514
10.1128/jvi.01752-22
url https://www.arca.fiocruz.br/handle/icict/57913
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/57913/2/JairoTemerozo_Dumithabib_etal_IOC_2023.pdf
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