Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.

Moens, Britta; Pannecouque, Christophe; López, Giovanni; Talledo, Michael; Gotuzzo, Eduardo; Cunha, Antonio Ricardo Khouri; Bittencourt, Achilea Candida Lisboa; Vallve, Maria de Lourdes Farre; Castro Filho, Bernardo Galvão; Vandamme, Anne-Mieke; Van Weyenbergh, Johan Jozef Rosa Maria

Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.

Detalhes bibliográficos
Autor(a) principal:	Moens, Britta
Data de Publicação:	2012
Outros Autores:	Pannecouque, Christophe, López, Giovanni, Talledo, Michael, Gotuzzo, Eduardo, Cunha, Antonio Ricardo Khouri, Bittencourt, Achilea Candida Lisboa, Vallve, Maria de Lourdes Farre, Castro Filho, Bernardo Galvão, Vandamme, Anne-Mieke, Van Weyenbergh, Johan Jozef Rosa Maria
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/8793
Resumo:	Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium

Metadados do item

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spelling	Moens, BrittaPannecouque, ChristopheLópez, GiovanniTalledo, MichaelGotuzzo, EduardoCunha, Antonio Ricardo KhouriBittencourt, Achilea Candida LisboaVallve, Maria de Lourdes FarreCastro Filho, Bernardo GalvãoVandamme, Anne-MiekeVan Weyenbergh, Johan Jozef Rosa Maria2014-11-11T19:09:55Z2014-11-11T19:09:55Z2012MOENS, B. et al. Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. Virology Journal, v. 9, p. 171, 2012.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/879310.1186/1743-422X-9-171engBioMed CentralSimultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, BelgiumRega Institute for Medical Research. Laboratory for Virology and Chemotherapy K.U.Leuven. Leuven, BelgiumUniversidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, PeruUniversidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, PeruUniversidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru / Universidad Peruana Cayetano Heredia. Facultad de Medicina. Departamento de Medicina. Lima, PeruRega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Professor Edgar Santos Teaching Hospital. Department of Pathology. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Bahia School of Medicine and Public Health. Salvador, BA, BrasilRega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Centro de Malária e outras Doenças Tropicais. Lisboa, PortugalRega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institute for Immunological Investigation iii-INCT. São Paulo, SP, BrasilBACKGROUND: IFN-Î± contributes extensively to host immune response upon viral infection through antiviral, pro-apoptotic, antiproliferative and immunomodulatory activities. Although extensively documented in various types of human cancers and viral infections, controversy exists in the exact mechanism of action of IFN-Î± in human immunodeficiency virus type 1 (HIV-1) and human T-lymphotropic virus type 1 (HTLV-1) retroviral infections. RESULTS: IFN-Î± displayed strong anti-HIV-1 effects in HIV-1/HTLV-1 co-infected MT-4 cells in vitro, demonstrated by the dose-dependent inhibition of the HIV-1-induced cytopathic effect (IC50 = 83.5 IU/ml, p < 0.0001) and p24 levels in cell-free supernatant (IC50 = 1.2 IU/ml, p < 0.0001). In contrast, IFN-Î± treatment did not affect cell viability or HTLV-1 viral mRNA levels in HTLV-1 mono-infected cell lines, based on flow cytometry and nCounter analysis, respectively. However, we were able to confirm the previously described post-transcriptional inhibition of HTLV-1 p19 secretion by IFN-Î± in cell lines (p = 0.0045), and extend this finding to primary Adult T cell Leukemia patient samples (p = 0.031). In addition, through microarray and nCounter analysis, we performed the first genome-wide simultaneous quantification of complete human and retroviral transciptomes, demonstrating significant transcriptional activation of interferon-stimulated genes without concomitant decrease of HTLV-1 mRNA levels. CONCLUSIONS: Taken together, our results indicate that both the absence of in vitro antiproliferative and pro-apoptotic activity as well as the modest post-transcriptional antiviral activity of IFN-Î± against HTLV-1, were not due to a cell-intrinsic defect in IFN-Î± signalisation, but rather represents a retrovirus-specific phenomenon, considering the strong HIV-1 inhibition in co-infected cells.RetrovirusIFN-αHIV-1HTLV-1IFN-α signalingAntiviral activityLinfócitos T CD4-Positivos/imunologiaLinfócitos T CD4-Positivos/virologiaVírus 1 Linfotrópico T Humano/imunologiaInterferon-alfa/biossínteseTransdução de SinalSobrevivência CelularPerfilação da Expressão GênicaHIV-1/imunologiaHumanosRNA/biossínteseRNA/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8793/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMoens B Simultaneous....pdfMoens B Simultaneous....pdfapplication/pdf1561034https://www.arca.fiocruz.br/bitstream/icict/8793/2/Moens%20B%20Simultaneous....pdf891e9491b8c19acf3073a5c4c4d4eecfMD52TEXTMoens B Simultaneous....pdf.txtMoens B Simultaneous....pdf.txtExtracted texttext/plain64660https://www.arca.fiocruz.br/bitstream/icict/8793/3/Moens%20B%20Simultaneous....pdf.txt8fb5aa67de85deabbf368f26744737bcMD53icict/87932023-03-15 14:33:27.094oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
title	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
spellingShingle	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. Moens, Britta Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity Linfócitos T CD4-Positivos/imunologia Linfócitos T CD4-Positivos/virologia Vírus 1 Linfotrópico T Humano/imunologia Interferon-alfa/biossíntese Transdução de Sinal Sobrevivência Celular Perfilação da Expressão Gênica HIV-1/imunologia Humanos RNA/biossíntese RNA/genética
title_short	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
title_full	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
title_fullStr	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
title_full_unstemmed	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
title_sort	Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.
author	Moens, Britta
author_facet	Moens, Britta Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antonio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Vallve, Maria de Lourdes Farre Castro Filho, Bernardo Galvão Vandamme, Anne-Mieke Van Weyenbergh, Johan Jozef Rosa Maria
author_role	author
author2	Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antonio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Vallve, Maria de Lourdes Farre Castro Filho, Bernardo Galvão Vandamme, Anne-Mieke Van Weyenbergh, Johan Jozef Rosa Maria
author2_role	author author author author author author author author author author
dc.contributor.author.fl_str_mv	Moens, Britta Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antonio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Vallve, Maria de Lourdes Farre Castro Filho, Bernardo Galvão Vandamme, Anne-Mieke Van Weyenbergh, Johan Jozef Rosa Maria
dc.subject.en.pt_BR.fl_str_mv	Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity
topic	Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity Linfócitos T CD4-Positivos/imunologia Linfócitos T CD4-Positivos/virologia Vírus 1 Linfotrópico T Humano/imunologia Interferon-alfa/biossíntese Transdução de Sinal Sobrevivência Celular Perfilação da Expressão Gênica HIV-1/imunologia Humanos RNA/biossíntese RNA/genética
dc.subject.decs.pt_BR.fl_str_mv	Linfócitos T CD4-Positivos/imunologia Linfócitos T CD4-Positivos/virologia Vírus 1 Linfotrópico T Humano/imunologia Interferon-alfa/biossíntese Transdução de Sinal Sobrevivência Celular Perfilação da Expressão Gênica HIV-1/imunologia Humanos RNA/biossíntese RNA/genética
description	Rega Institute for Medical Research,Laboratory of Clinical and Epidemiological Virology K.U.Leuven. Leuven, Belgium
publishDate	2012
dc.date.issued.fl_str_mv	2012
dc.date.accessioned.fl_str_mv	2014-11-11T19:09:55Z
dc.date.available.fl_str_mv	2014-11-11T19:09:55Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	MOENS, B. et al. Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. Virology Journal, v. 9, p. 171, 2012.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/8793
dc.identifier.issn.none.fl_str_mv	1743-422X
dc.identifier.doi.none.fl_str_mv	10.1186/1743-422X-9-171
identifier_str_mv	MOENS, B. et al. Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines. Virology Journal, v. 9, p. 171, 2012. 1743-422X 10.1186/1743-422X-9-171
url	https://www.arca.fiocruz.br/handle/icict/8793
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines.

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