Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51662 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil/Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil. |
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Lanna, Mariana FerreiraResende, Lucilene AparecidaSoares, Rodrigo Dian de Oliveira AguiarMiranda, Marina Barcelos deMendonça, Ludmila Zanandreis deMelo Júnior, Otoni Alves de OliveiraMariano, Reysla Maria da SilveiraLeite, Jaqueline CostaSilveira, PatriciaOliveira, Rodrigo CorrêaDutra, Walderez OrnelasReis, Alexandre BarbosaMartins Filho, Olindo AssisMoura, Sandra Aparecida Lima deLemos, Denise SilveiraGiunchetti, Rodolfo Cordeiro2022-03-09T17:35:23Z2022-03-09T17:35:23Z2020LANNA, Mariana Ferreira et al. Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes. Front Bioeng Biotechnol., v. 8:538203, 2020. doi: 10.3389/fbioe.2020.538203.2296-4185https://www.arca.fiocruz.br/handle/icict/5166210.3389/fbioe.2020.538203engFrontiers Media S.AKinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil/Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil/Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.Instituto de Ciências Exatas e Biológicas. Laboratório de Biomateriais e Patologia Experimental. Ouro Preto, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil.Instituto de Ciências Exatas e Biológicas. Laboratório de Biomateriais e Patologia Experimental. Ouro Preto, Brazil.Universidade José Rosário Vellano. Departamento de Medicina. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil.The sponge implant has been applied as an important in vivo model for the study of inflammatory processes as it induces the migration, proliferation, and accumulation of inflammatory cells, angiogenesis, and extracellular matrix deposition in its trabeculae. The characterization of immune events in sponge implants would be useful in identifying the immunological events that could support the selection of an appropriate experimental model (mouse strain) and time post-implant analysis in optimized protocols for novel applications of this model such as in biomolecules screening. Here, the changes in histological/morphometric, immunophenotypic and functional features of infiltrating leukocytes (LEU) were assessed in sponge implants for Swiss, BALB/c, and C57BL/6 mice. A gradual increase of fibrovascular stroma and a progressive decrease in LEU infiltration, mainly composed of polymorphonuclear cells with progressive shift toward mononuclear cells at late time-points were observed over time. Usually, Swiss mice presented a more prominent immune response with late mixed pattern (pro-inflammatory/anti-inflammatory: IL-2/IFN-γ/IL-4/IL-10/IL-17) of cytokine production. While BALB/c mice showed an early activation of the innate response with a controlled cytokine profile (low inflammatory potential), C57BL/6 mice presented a typical early pro-inflammatory (IL-6/TNF/IFN-γ) response with persistent neutrophilic involvement. A rational selection of the ideal time-point/mouse-lineage would avoid bias or tendentious results. Criteria such as low number of increased biomarkers, no recruitment of cytotoxic response, minor cytokine production, and lower biomarker connectivity (described as biomarker signature analysis and network analysis) guided the choice of the best time-point for each model (Day5/Swiss; Day7/BALB/c; Day6/C57BL/6) with wide application for screening purposes, such as identification of therapeutic biomolecules, selection of antigens/adjuvants, and follow-up of innate and adaptive immune response to vaccines candidates.biomolecules screeningcytokinesdynamics of phenotypic and functional featuresimmunophenotypingsponge implant modelinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51662/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALKinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants.pdfKinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants.pdfapplication/pdf6763313https://www.arca.fiocruz.br/bitstream/icict/51662/2/Kinetics%20of%20Phenotypic%20and%20Functional%20Changes%20in%20Mouse%20Models%20of%20Sponge%20Implants.pdf5940e1e898737dc5a66785992edfbecdMD52icict/516622022-03-09 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| dc.title.pt_BR.fl_str_mv |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| title |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| spellingShingle |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes Lanna, Mariana Ferreira biomolecules screening cytokines dynamics of phenotypic and functional features immunophenotyping sponge implant model |
| title_short |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| title_full |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| title_fullStr |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| title_full_unstemmed |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| title_sort |
Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes |
| author |
Lanna, Mariana Ferreira |
| author_facet |
Lanna, Mariana Ferreira Resende, Lucilene Aparecida Soares, Rodrigo Dian de Oliveira Aguiar Miranda, Marina Barcelos de Mendonça, Ludmila Zanandreis de Melo Júnior, Otoni Alves de Oliveira Mariano, Reysla Maria da Silveira Leite, Jaqueline Costa Silveira, Patricia Oliveira, Rodrigo Corrêa Dutra, Walderez Ornelas Reis, Alexandre Barbosa Martins Filho, Olindo Assis Moura, Sandra Aparecida Lima de Lemos, Denise Silveira Giunchetti, Rodolfo Cordeiro |
| author_role |
author |
| author2 |
Resende, Lucilene Aparecida Soares, Rodrigo Dian de Oliveira Aguiar Miranda, Marina Barcelos de Mendonça, Ludmila Zanandreis de Melo Júnior, Otoni Alves de Oliveira Mariano, Reysla Maria da Silveira Leite, Jaqueline Costa Silveira, Patricia Oliveira, Rodrigo Corrêa Dutra, Walderez Ornelas Reis, Alexandre Barbosa Martins Filho, Olindo Assis Moura, Sandra Aparecida Lima de Lemos, Denise Silveira Giunchetti, Rodolfo Cordeiro |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lanna, Mariana Ferreira Resende, Lucilene Aparecida Soares, Rodrigo Dian de Oliveira Aguiar Miranda, Marina Barcelos de Mendonça, Ludmila Zanandreis de Melo Júnior, Otoni Alves de Oliveira Mariano, Reysla Maria da Silveira Leite, Jaqueline Costa Silveira, Patricia Oliveira, Rodrigo Corrêa Dutra, Walderez Ornelas Reis, Alexandre Barbosa Martins Filho, Olindo Assis Moura, Sandra Aparecida Lima de Lemos, Denise Silveira Giunchetti, Rodolfo Cordeiro |
| dc.subject.en.pt_BR.fl_str_mv |
biomolecules screening cytokines dynamics of phenotypic and functional features immunophenotyping sponge implant model |
| topic |
biomolecules screening cytokines dynamics of phenotypic and functional features immunophenotyping sponge implant model |
| description |
Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, Brazil/Laboratório de Pesquisas Clínicas, Programa de Pós-Graduação de Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil. |
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2020 |
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2020 |
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2022-03-09T17:35:23Z |
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2022-03-09T17:35:23Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
LANNA, Mariana Ferreira et al. Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes. Front Bioeng Biotechnol., v. 8:538203, 2020. doi: 10.3389/fbioe.2020.538203. |
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https://www.arca.fiocruz.br/handle/icict/51662 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2296-4185 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fbioe.2020.538203 |
| identifier_str_mv |
LANNA, Mariana Ferreira et al. Kinetics of Phenotypic and Functional Changes in Mouse Models of Sponge Implants: Rational Selection to Optimize Protocols for Specific Biomolecules Screening Purposes. Front Bioeng Biotechnol., v. 8:538203, 2020. doi: 10.3389/fbioe.2020.538203. 2296-4185 10.3389/fbioe.2020.538203 |
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eng |
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eng |
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Frontiers Media S.A |
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Frontiers Media S.A |
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