Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Freitas e Silva, Rafael de
Data de Publicação: 2014
Outros Autores: Santos, Neyliane Frassinetti Gonçalves Dos, Pereira, Valéria Rěgo Alves, Amaral, Ademir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23366
Resumo: Universidade Federal de Pernambuco. Laboratório de Modelagem e Biodosimetria Aplicada (LAMBDA). Recife, PE, Brasil / Universidade de Pernambuco. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
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spelling Freitas e Silva, Rafael deSantos, Neyliane Frassinetti Gonçalves DosPereira, Valéria Rěgo AlvesAmaral, Ademir2017-11-27T14:02:08Z2017-11-27T14:02:08Z2014FREITAS e SILVA, R. de et al. Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry. Dose-Response: A Publication of International Hormesis Society, v. 12, n. 1, p. 110–120, jan. 2014.1559-3258https://www.arca.fiocruz.br/handle/icict/2336610.2203/dose-response.13-015.SilvaengProteína Supressora de Tumor p53Tolerância a RadiaçãoCitometria de Fluxotaxa de proliferaçãoCFSEP53 proteinProliferation rateFlow CytometryCFSERadiosensitivityProliferação CelularProteína Supressora de Tumor p53DNAbioindicadorescitometria de fluxoSimultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pernambuco. Laboratório de Modelagem e Biodosimetria Aplicada (LAMBDA). Recife, PE, Brasil / Universidade de Pernambuco. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Modelagem e Biodosimetria Aplicada (LAMBDA). Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Modelagem e Biodosimetria Aplicada (LAMBDA). Recife, PE, Brasil.P53 protein has an intrinsic role in modulating the cellular response against DNA radioinduced damages and has been pointed out as an indirect indicator of individual radiosensitivity. The rate of cell proliferation is also a parameter that has been related to tissue sensitivity to radiation. However, this feature is yet understudied. In this context, the aim of this work was to employ Flow Cytometry (FC) for simultaneously assessing of p53 protein expression levels together with cellular proliferation rate of irradiated human lymphocytes. From in vitro irradiated human blood samples, mononuclear cells were isolated and labeled with Carboxylfluorescein Diacetate Succinimidyl Ester (CFSE) prior to phytohaemagglutinin (PHA) stimulation in culture for 96 hours. Cells were also labeled with anti-p53 monoclonal antibody PE-conjugated in order to analyze either proliferation rate or p53 expression levels by FC. It was verified a reduction in the proliferation rate of irradiated lymphocytes and, in parallel, a rise in the p53 expression levels, similar for quiescent and proliferating lymphocytes. The results emphasize the importance of the use of CFSE-stained lymphocytes in assays associated to proliferation rate and the use of this methodology in several studies, such as for evaluating individual radiosensitivity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23366/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. SIMULTANEOUS ANALYSIS - freitas e silva.pdfart. SIMULTANEOUS ANALYSIS - freitas e silva.pdfapplication/pdf2005089https://www.arca.fiocruz.br/bitstream/icict/23366/2/art.%20SIMULTANEOUS%20ANALYSIS%20-%20freitas%20e%20silva.pdfb7ecc361c6291f2c943cb4398eea74b9MD52TEXTart. SIMULTANEOUS ANALYSIS - freitas e silva.pdf.txtart. 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dc.title.pt_BR.fl_str_mv Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
title Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
spellingShingle Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
Freitas e Silva, Rafael de
Proteína Supressora de Tumor p53
Tolerância a Radiação
Citometria de Fluxo
taxa de proliferação
CFSE
P53 protein
Proliferation rate
Flow Cytometry
CFSE
Radiosensitivity
Proliferação Celular
Proteína Supressora de Tumor p53
DNA
bioindicadores
citometria de fluxo
title_short Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
title_full Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
title_fullStr Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
title_full_unstemmed Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
title_sort Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry
author Freitas e Silva, Rafael de
author_facet Freitas e Silva, Rafael de
Santos, Neyliane Frassinetti Gonçalves Dos
Pereira, Valéria Rěgo Alves
Amaral, Ademir
author_role author
author2 Santos, Neyliane Frassinetti Gonçalves Dos
Pereira, Valéria Rěgo Alves
Amaral, Ademir
author2_role author
author
author
dc.contributor.author.fl_str_mv Freitas e Silva, Rafael de
Santos, Neyliane Frassinetti Gonçalves Dos
Pereira, Valéria Rěgo Alves
Amaral, Ademir
dc.subject.other.pt_BR.fl_str_mv Proteína Supressora de Tumor p53
Tolerância a Radiação
Citometria de Fluxo
taxa de proliferação
CFSE
topic Proteína Supressora de Tumor p53
Tolerância a Radiação
Citometria de Fluxo
taxa de proliferação
CFSE
P53 protein
Proliferation rate
Flow Cytometry
CFSE
Radiosensitivity
Proliferação Celular
Proteína Supressora de Tumor p53
DNA
bioindicadores
citometria de fluxo
dc.subject.en.pt_BR.fl_str_mv P53 protein
Proliferation rate
Flow Cytometry
CFSE
Radiosensitivity
dc.subject.decs.pt_BR.fl_str_mv Proliferação Celular
Proteína Supressora de Tumor p53
DNA
bioindicadores
citometria de fluxo
description Universidade Federal de Pernambuco. Laboratório de Modelagem e Biodosimetria Aplicada (LAMBDA). Recife, PE, Brasil / Universidade de Pernambuco. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2017-11-27T14:02:08Z
dc.date.available.fl_str_mv 2017-11-27T14:02:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREITAS e SILVA, R. de et al. Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry. Dose-Response: A Publication of International Hormesis Society, v. 12, n. 1, p. 110–120, jan. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23366
dc.identifier.issn.pt_BR.fl_str_mv 1559-3258
dc.identifier.doi.none.fl_str_mv 10.2203/dose-response.13-015.Silva
identifier_str_mv FREITAS e SILVA, R. de et al. Simultaneous analysis of p53 protein expression and cell proliferation in irradiated human lymphocytes by flow cytometry. Dose-Response: A Publication of International Hormesis Society, v. 12, n. 1, p. 110–120, jan. 2014.
1559-3258
10.2203/dose-response.13-015.Silva
url https://www.arca.fiocruz.br/handle/icict/23366
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23366/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23366/2/art.%20SIMULTANEOUS%20ANALYSIS%20-%20freitas%20e%20silva.pdf
https://www.arca.fiocruz.br/bitstream/icict/23366/3/art.%20SIMULTANEOUS%20ANALYSIS%20-%20freitas%20e%20silva.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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