A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains

Detalhes bibliográficos
Autor(a) principal: Bernardes-Engemann, A. R.
Data de Publicação: 2009
Outros Autores: Loureiro y Penha, C. V., Benvenuto, F., Braga, J. U., Barros, M. B. L., Orofino Costa, R., Lopes-Bezerra, L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33500
Resumo: 2020-06-13
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spelling Bernardes-Engemann, A. R.Loureiro y Penha, C. V.Benvenuto, F.Braga, J. U.Barros, M. B. L.Orofino Costa, R.Lopes-Bezerra, L.2019-06-13T14:57:48Z2019-06-13T14:57:48Z2009BERNARDES-ENGERMANN, Andréa R. et al. A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains. Medical Mycology, v. 47, p. 874-878, Dec. 2009.1369-3786https://www.arca.fiocruz.br/handle/icict/3350010.3109/136937808026955201460-2709engOxford University PressA comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-06-13Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Pós-Graduação em Fisiopatologia Clínica e Experimental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Micologia Celular e Proteômica. Rio de Janeiro, RJ, Brasil.A previous work showed that an antigenic fraction of Sporothrix schenckii , Ss CBF, was specifi cally recognized by IgG antibodies present in patients’ clinical specimens. The objective of this investigation was to compare the reactivity of this antigen recovered from three S. schenckii strains. ROC curve analysis revealed a variation in the sensitivity and specifi city of the antigen derived from each of the strains, with a higher AUC for strain 1099-18. Moreover, the presence of the main O-glycosidically-linked epitopes described in the Ss CBF fraction was ascertained. A signifi cant reduction in Ss CBF reactivity of all the strains was observed after β-elimination confi rming the presence of O-glycan epitopes. The antigen isolated from strain 1099-18 proved to be a more accurate diagnostic tool for serodiagnosis of sporotrichosis.SporotrichosisSerologySporothrix schenckiiSsCBF antigenROC analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33500/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Bernardes_Engamann_Andréa_etal_INI_2009.pdfve_Bernardes_Engamann_Andréa_etal_INI_2009.pdfapplication/pdf364832https://www.arca.fiocruz.br/bitstream/icict/33500/2/ve_Bernardes_Engamann_Andr%c3%a9a_etal_INI_2009.pdf836cc47420e6f2e0e152b318d2aceb80MD52TEXTve_Bernardes_Engamann_Andréa_etal_INI_2009.pdf.txtve_Bernardes_Engamann_Andréa_etal_INI_2009.pdf.txtExtracted texttext/plain19647https://www.arca.fiocruz.br/bitstream/icict/33500/3/ve_Bernardes_Engamann_Andr%c3%a9a_etal_INI_2009.pdf.txt31d577ef1dbcf2a81e7f3f181b4f0058MD53icict/335002021-03-24 16:32:32.92oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
title A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
spellingShingle A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
Bernardes-Engemann, A. R.
Sporotrichosis
Serology
Sporothrix schenckii
SsCBF antigen
ROC analysis
title_short A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
title_full A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
title_fullStr A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
title_full_unstemmed A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
title_sort A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains
author Bernardes-Engemann, A. R.
author_facet Bernardes-Engemann, A. R.
Loureiro y Penha, C. V.
Benvenuto, F.
Braga, J. U.
Barros, M. B. L.
Orofino Costa, R.
Lopes-Bezerra, L.
author_role author
author2 Loureiro y Penha, C. V.
Benvenuto, F.
Braga, J. U.
Barros, M. B. L.
Orofino Costa, R.
Lopes-Bezerra, L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bernardes-Engemann, A. R.
Loureiro y Penha, C. V.
Benvenuto, F.
Braga, J. U.
Barros, M. B. L.
Orofino Costa, R.
Lopes-Bezerra, L.
dc.subject.en.pt_BR.fl_str_mv Sporotrichosis
Serology
Sporothrix schenckii
SsCBF antigen
ROC analysis
topic Sporotrichosis
Serology
Sporothrix schenckii
SsCBF antigen
ROC analysis
description 2020-06-13
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2019-06-13T14:57:48Z
dc.date.available.fl_str_mv 2019-06-13T14:57:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BERNARDES-ENGERMANN, Andréa R. et al. A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains. Medical Mycology, v. 47, p. 874-878, Dec. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33500
dc.identifier.issn.pt_BR.fl_str_mv 1369-3786
dc.identifier.doi.none.fl_str_mv 10.3109/13693780802695520
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv BERNARDES-ENGERMANN, Andréa R. et al. A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains. Medical Mycology, v. 47, p. 874-878, Dec. 2009.
1369-3786
10.3109/13693780802695520
1460-2709
url https://www.arca.fiocruz.br/handle/icict/33500
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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