Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus

Detalhes bibliográficos
Autor(a) principal: Pereira, Jéssica
Data de Publicação: 2020
Outros Autores: Diogo, Calebe, Fonseca, Arlene, Bomfim, Larissa, Cardoso, Pedro, Santos, Anna, Dittz, Uilla, Miranda, Kildare, Souza, Wanderley de, Gioda, Adriana, Calderon, Enrique R. D., Araripe, Luciana, Bruno, Rafaela, Ramos, Isabela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41473
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.
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spelling Pereira, JéssicaDiogo, CalebeFonseca, ArleneBomfim, LarissaCardoso, PedroSantos, AnnaDittz, UillaMiranda, KildareSouza, Wanderley deGioda, AdrianaCalderon, Enrique R. D.Araripe, LucianaBruno, RafaelaRamos, Isabela2020-06-01T16:52:16Z2020-06-01T16:52:16Z2020PEREIRA, Jéssica et al. Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus. PLoS Neglected Tropical Diseases, v. 14, n. 1, p. 1-20, Jan. 2020.1935-2727https://www.arca.fiocruz.br/handle/icict/4147310.1371/journal.pntd.00080121935-2735engPublic Libray of ScienceRhodnius prolixusBiogêneseFolicular atresiaInseto vetorRpATG8Rhodnius prolixusBiogenesisRpATG8Insect vetorFolicular atresiaSilencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.Unversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil.Pontifícia Universidade Católica do Rio de Janeiro. Departamento de Química. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Follicular atresia is the mechanism by which the oocyte contents are degraded during oogenesis in response to stress conditions, allowing the energetic resources stored in the developing oocytes to be reallocated to optimize female fitness. Autophagy is a conserved intracellular degradation pathway where double-membrane vesicles are formed around target organelles leading to their degradation after lysosome fusion. The autophagy-related protein 8 (ATG8) is conjugated to the autophagic membrane and has a key role in the elongation and closure of the autophagosome. Here we identified one single isoform of ATG8 in the genome of the insect vector of Chagas Disease Rhodnius prolixus (RpATG8) and found that it is highly expressed in the ovary during vitellogenesis. Accordingly, autophagosomes were detected in the vitellogenic oocytes, as seen by immunoblotting and electron microscopy. To test if autophagosomes were important for follicular atresia, we silenced RpATG8 and elicited atresia in vitellogenic females by Zymosan-A injections. We found that silenced females were still able to trigger the same levels of follicle atresia, and that their atretic oocytes presented a characteristic morphology, with accumulated brown aggregates. Regardless of the difference in morphology, RpATG8-silenced atretic oocytes presented the same levels of protein, TAG and PolyP, as detected in control atretic oocytes, as well as the same levels of acidification of the yolk organelles. Because follicular atresia has the ultimate goal of restoring female fitness, we tested if RpATG8-silenced atresia would result in female physiology and behavior changes. Under insectarium conditions, we found that atresia- induced control and RpATG8-silenced females present no changes in blood meal digestion, survival, oviposition, TAG content in the fat body, haemolymph amino acid levels and overall locomotor activity. Altogether, we found that autophagosomes are formed during oogenesis and that the silencing of RpATG8 impairs autophagosome biogenesis in the oocytes. Nevertheless, regarding major macromolecule degradation and adaptations to the fitness costs imposed by triggering an immune response, we found that autophagic organelles are not essential for follicle atresia in R. prolixus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41473/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLucianaAraripe_RafaelaBruno_etal_IOC_2020.pdfLucianaAraripe_RafaelaBruno_etal_IOC_2020.pdfapplication/pdf3676122https://www.arca.fiocruz.br/bitstream/icict/41473/2/LucianaAraripe_RafaelaBruno_etal_IOC_2020.pdf4c4e3aa9e9a04d522dc77dcaf64ac14cMD52TEXTLucianaAraripe_RafaelaBruno_etal_IOC_2020.pdf.txtLucianaAraripe_RafaelaBruno_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
title Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
spellingShingle Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
Pereira, Jéssica
Rhodnius prolixus
Biogênese
Folicular atresia
Inseto vetor
RpATG8
Rhodnius prolixus
Biogenesis
RpATG8
Insect vetor
Folicular atresia
title_short Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
title_full Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
title_fullStr Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
title_full_unstemmed Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
title_sort Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus
author Pereira, Jéssica
author_facet Pereira, Jéssica
Diogo, Calebe
Fonseca, Arlene
Bomfim, Larissa
Cardoso, Pedro
Santos, Anna
Dittz, Uilla
Miranda, Kildare
Souza, Wanderley de
Gioda, Adriana
Calderon, Enrique R. D.
Araripe, Luciana
Bruno, Rafaela
Ramos, Isabela
author_role author
author2 Diogo, Calebe
Fonseca, Arlene
Bomfim, Larissa
Cardoso, Pedro
Santos, Anna
Dittz, Uilla
Miranda, Kildare
Souza, Wanderley de
Gioda, Adriana
Calderon, Enrique R. D.
Araripe, Luciana
Bruno, Rafaela
Ramos, Isabela
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Jéssica
Diogo, Calebe
Fonseca, Arlene
Bomfim, Larissa
Cardoso, Pedro
Santos, Anna
Dittz, Uilla
Miranda, Kildare
Souza, Wanderley de
Gioda, Adriana
Calderon, Enrique R. D.
Araripe, Luciana
Bruno, Rafaela
Ramos, Isabela
dc.subject.other.pt_BR.fl_str_mv Rhodnius prolixus
Biogênese
Folicular atresia
Inseto vetor
RpATG8
topic Rhodnius prolixus
Biogênese
Folicular atresia
Inseto vetor
RpATG8
Rhodnius prolixus
Biogenesis
RpATG8
Insect vetor
Folicular atresia
dc.subject.en.pt_BR.fl_str_mv Rhodnius prolixus
Biogenesis
RpATG8
Insect vetor
Folicular atresia
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Laboratório de Bioquímica de Insetos. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-06-01T16:52:16Z
dc.date.available.fl_str_mv 2020-06-01T16:52:16Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Jéssica et al. Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus. PLoS Neglected Tropical Diseases, v. 14, n. 1, p. 1-20, Jan. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41473
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0008012
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv PEREIRA, Jéssica et al. Silencing of RpATG8 impairs the biogenesis of maternal autophagosomes in vitellogenic oocytes, but does not interrupt follicular atresia in the insect vector Rhodnius prolixus. PLoS Neglected Tropical Diseases, v. 14, n. 1, p. 1-20, Jan. 2020.
1935-2727
10.1371/journal.pntd.0008012
1935-2735
url https://www.arca.fiocruz.br/handle/icict/41473
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Libray of Science
publisher.none.fl_str_mv Public Libray of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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