Sepsis induces brain mitochondrial dysfunction

Detalhes bibliográficos
Autor(a) principal: d’Avila, Joana da Costa P.
Data de Publicação: 2008
Outros Autores: Santiago, Ana Paula S. A., Amâncio, Rodrigo T., Galina, Antonio, Oliveira, Marcus F., Bozza, Fernando A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57597
Resumo: Supported, in part, by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and DECIT/Ministério da Saúde (MS) through Edital Acidentes e Trauma 2004, CNPq through Edital Universal 2003 and 2006, Fundação Universitáriã José Bonifácio (FUJB, Brazil) through Prêmio Antônio Luiz Vianna 2004 and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil) through APQ-1 and Fundação Oswaldo Cruz - PAPES IV (Brazil). Drs. Oliveira and Galina are research scholars from CNPq.
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spelling d’Avila, Joana da Costa P.Santiago, Ana Paula S. A.Amâncio, Rodrigo T.Galina, AntonioOliveira, Marcus F.Bozza, Fernando A.2023-03-27T18:54:26Z2023-03-27T18:54:26Z2008D’AVILA, Joana da Costa P. et al. Sepsis induces brain mitochondrial dysfunction. Critical Care Medicine, v. 36, n. 6, p. 1925-1932, Jun. 2008.0090-3493https://www.arca.fiocruz.br/handle/icict/5759710.1097/CCM.0b013e3181760c4b1530-0293Supported, in part, by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and DECIT/Ministério da Saúde (MS) through Edital Acidentes e Trauma 2004, CNPq through Edital Universal 2003 and 2006, Fundação Universitáriã José Bonifácio (FUJB, Brazil) through Prêmio Antônio Luiz Vianna 2004 and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil) through APQ-1 and Fundação Oswaldo Cruz - PAPES IV (Brazil). Drs. Oliveira and Galina are research scholars from CNPq.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica Redox. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioenergética Adaptativa. Programa de Biofísica e Bioquímica Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioenergética Adaptativa. Programa de Biofísica e Bioquímica Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Bioquímica Redox. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas Rio de Janeiro, RJ, Brasil.Objective: Mitochondrial dysfunctions have been associated with the pathogenesis of sepsis. A systematic survey of mitochondrial function in brain tissues during sepsis is lacking. In the present work, we investigate brain mitochondrial function in a septic mouse model. Design: Prospective animal study. Setting: University research laboratory. Subjects: Male Swiss mice, aged 6-8 wks. Interventions: Mice were subjected to cecal ligation and perforation (sepsis group) with saline resuscitation or to sham operation (control group). Measurements and main results: Oxygen consumption was measured polarographically in an oximeter. Brain homogenates from septic animals presented higher oxygen consumption in the absence of adenosine 5'-diphosphate (state 4) compared with control animals. The increase in state 4 respiration in animals in the cecal ligation and perforation group resulted in a drastic decrease in both respiratory control and adenosine 5'-diphosphate/oxygen ratios, indicating a reduction in the oxidative phosphorylation efficiency. Septic animals presented a significant increase in the recovery time of mitochondrial membrane potential on adenosine 5'-diphosphate addition compared with control animals, suggesting a proton leak through the inner mitochondrial membrane. The septic group presented a general reduction in the content of cytochromes. Moreover, the activity of cytochrome c oxidase was specifically and significantly decreased in the brain during sepsis. Hydrogen peroxide generation by brain mitochondria from septic mice did not respond to substrates of electron transport chain or to adenosine 5'-diphosphate, showing that mitochondrial function may be compromised in a critical level in the brain during sepsis. Conclusions: The mitochondrial dysfunctions demonstrated here indicate that uncoupling of oxidative phosphorylation takes place in the brain of septic mice, compromising tissue bioenergetic efficiency.engSociety of Critical Care MedicineSepsis induces brain mitochondrial dysfunctioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMitochondrial dysfunctionsPathogenesis of sepsisBrainBrain mitochondrial functioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_d’Avila_Joana_etal_INI_2008.pdfve_d’Avila_Joana_etal_INI_2008.pdfapplication/pdf525904https://www.arca.fiocruz.br/bitstream/icict/57597/2/ve_d%e2%80%99Avila_Joana_etal_INI_2008.pdfb426208b34cf4239be39b15392c318bfMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57597/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/575972023-03-27 16:14:05.895oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-27T19:14:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Sepsis induces brain mitochondrial dysfunction
title Sepsis induces brain mitochondrial dysfunction
spellingShingle Sepsis induces brain mitochondrial dysfunction
d’Avila, Joana da Costa P.
Mitochondrial dysfunctions
Pathogenesis of sepsis
Brain
Brain mitochondrial function
title_short Sepsis induces brain mitochondrial dysfunction
title_full Sepsis induces brain mitochondrial dysfunction
title_fullStr Sepsis induces brain mitochondrial dysfunction
title_full_unstemmed Sepsis induces brain mitochondrial dysfunction
title_sort Sepsis induces brain mitochondrial dysfunction
author d’Avila, Joana da Costa P.
author_facet d’Avila, Joana da Costa P.
Santiago, Ana Paula S. A.
Amâncio, Rodrigo T.
Galina, Antonio
Oliveira, Marcus F.
Bozza, Fernando A.
author_role author
author2 Santiago, Ana Paula S. A.
Amâncio, Rodrigo T.
Galina, Antonio
Oliveira, Marcus F.
Bozza, Fernando A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv d’Avila, Joana da Costa P.
Santiago, Ana Paula S. A.
Amâncio, Rodrigo T.
Galina, Antonio
Oliveira, Marcus F.
Bozza, Fernando A.
dc.subject.en.en_US.fl_str_mv Mitochondrial dysfunctions
Pathogenesis of sepsis
Brain
Brain mitochondrial function
topic Mitochondrial dysfunctions
Pathogenesis of sepsis
Brain
Brain mitochondrial function
description Supported, in part, by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and DECIT/Ministério da Saúde (MS) through Edital Acidentes e Trauma 2004, CNPq through Edital Universal 2003 and 2006, Fundação Universitáriã José Bonifácio (FUJB, Brazil) through Prêmio Antônio Luiz Vianna 2004 and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil) through APQ-1 and Fundação Oswaldo Cruz - PAPES IV (Brazil). Drs. Oliveira and Galina are research scholars from CNPq.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2023-03-27T18:54:26Z
dc.date.available.fl_str_mv 2023-03-27T18:54:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv D’AVILA, Joana da Costa P. et al. Sepsis induces brain mitochondrial dysfunction. Critical Care Medicine, v. 36, n. 6, p. 1925-1932, Jun. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57597
dc.identifier.issn.en_US.fl_str_mv 0090-3493
dc.identifier.doi.none.fl_str_mv 10.1097/CCM.0b013e3181760c4b
dc.identifier.eissn.none.fl_str_mv 1530-0293
identifier_str_mv D’AVILA, Joana da Costa P. et al. Sepsis induces brain mitochondrial dysfunction. Critical Care Medicine, v. 36, n. 6, p. 1925-1932, Jun. 2008.
0090-3493
10.1097/CCM.0b013e3181760c4b
1530-0293
url https://www.arca.fiocruz.br/handle/icict/57597
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society of Critical Care Medicine
publisher.none.fl_str_mv Society of Critical Care Medicine
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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