Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells

Detalhes bibliográficos
Autor(a) principal: Thé, Torriceli Souza
Data de Publicação: 2013
Outros Autores: Portella, Renata Siqueira, Guerreiro, Marcos Lázaro, Andrade, Sonia Gumes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15203
Resumo: Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, Brasil
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spelling Thé, Torriceli SouzaPortella, Renata SiqueiraGuerreiro, Marcos LázaroAndrade, Sonia Gumes2016-08-08T14:22:45Z2016-08-08T14:22:45Z2013THÉ, T. S. et al. Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells. Memórias do Instituto Oswaldo Cruz, v. 108, n. 6, p. 691-698, 2013.0074-0276https://www.arca.fiocruz.br/handle/icict/1520310.1590/0074-0276108062013004engInstituto Oswaldo CruzEffect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, BrasilAcute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a potential explanation of the indeterminate form. We investigated the effect of cyclophosphamide (CYCL) treatment on the regulatory mechanism of DTH and the participation of heart interstitial dendritic cells (IDCs) in this process using BALB/c mice chronically infected with T. cruzi. One group was treated with CYCL (20 mg/kg body weight) for one month. A DTH skin test was performed by intradermal injection of T. cruzi antigen (3 mg/mL) in the hind-footpad and measured the skin thickness after 24 h, 48 h and 72 h. The skin test revealed increased thickness in antigen-injected footpads, which was more evident in the mice treated with CYCL than in those mice that did not receive treatment. The thickened regions were characterised by perivascular infiltrates and areas of necrosis. Intense lesions of the myocardium were present in three/16 cases and included large areas of necrosis. Morphometric evaluation of lymphocytes showed a predominance of TCD8 cells. Heart IDCs were immunolabelled with specific antibodies (CD11b and CD11c) and T. cruzi antigens were detected using a specific anti-T. cruzi antibody. Identification of T. cruzi antigens, sequestered in these cells using specific anti-T. cruzi antibodies was done, showing a significant increase in the number of these cells in treated mice. These results indicate that IDCs participate in the regulatory mechanisms of DTH response to T. cruzi infection.Trypanosoma cruziMurine infectionChronic myocarditisDelayed hypersensitivityCyclophosphamideHeart dendritic cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15203/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALThe TS Effect of treatment....pdfThe TS Effect of treatment....pdfapplication/pdf2161236https://www.arca.fiocruz.br/bitstream/icict/15203/2/The%20TS%20Effect%20of%20treatment....pdf4e052c125c7af743d9a26919fd21a139MD52TEXTThe TS Effect of treatment....pdf.txtThe TS Effect of treatment....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
title Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
spellingShingle Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
Thé, Torriceli Souza
Trypanosoma cruzi
Murine infection
Chronic myocarditis
Delayed hypersensitivity
Cyclophosphamide
Heart dendritic cells
title_short Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
title_full Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
title_fullStr Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
title_full_unstemmed Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
title_sort Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
author Thé, Torriceli Souza
author_facet Thé, Torriceli Souza
Portella, Renata Siqueira
Guerreiro, Marcos Lázaro
Andrade, Sonia Gumes
author_role author
author2 Portella, Renata Siqueira
Guerreiro, Marcos Lázaro
Andrade, Sonia Gumes
author2_role author
author
author
dc.contributor.author.fl_str_mv Thé, Torriceli Souza
Portella, Renata Siqueira
Guerreiro, Marcos Lázaro
Andrade, Sonia Gumes
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Murine infection
Chronic myocarditis
Delayed hypersensitivity
Cyclophosphamide
Heart dendritic cells
topic Trypanosoma cruzi
Murine infection
Chronic myocarditis
Delayed hypersensitivity
Cyclophosphamide
Heart dendritic cells
description Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença de Chagas Experimental, Autoimunidade e Imunidade Celular. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-08-08T14:22:45Z
dc.date.available.fl_str_mv 2016-08-08T14:22:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv THÉ, T. S. et al. Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells. Memórias do Instituto Oswaldo Cruz, v. 108, n. 6, p. 691-698, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15203
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276108062013004
identifier_str_mv THÉ, T. S. et al. Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells. Memórias do Instituto Oswaldo Cruz, v. 108, n. 6, p. 691-698, 2013.
0074-0276
10.1590/0074-0276108062013004
url https://www.arca.fiocruz.br/handle/icict/15203
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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