Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.

Detalhes bibliográficos
Autor(a) principal: Schriefer, Albert
Data de Publicação: 1999
Outros Autores: Maltez, J. R, Silva, N, Stoeckle, M.Y, Barral Netto, Manoel, Riley, Lee Woodland
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5652
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Schriefer, AlbertMaltez, J. RSilva, NStoeckle, M.YBarral Netto, ManoelRiley, Lee Woodland2012-10-05T18:51:53Z2012-10-05T18:51:53Z1999SCHRIEFER, A. et al. Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. Vaccine, v. 17, n. 7-8, p. 770-8, feb. 1999.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/5652engExpression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCornell University Medical College. Division of International Medicine and Infectious Diseases. New York, New YorkFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCornell University Medical College. Division of International Medicine and Infectious Diseases. New York, New YorkEnteropathogenic Escherichia coli (EPEC) is a major cause of childhood diarrhea in developing countries and is a leading cause of severe diarrheal illness among Brazilian infants. As one approach to constructing a vaccine candidate against diarrhea caused by EPEC, we evaluated whether the pilin subunit (BfpA) of the bundle-forming pilus (BFP) could be expressed by a live Salmonella vaccine strain. Several copies of the coding region of BfpA (bfpA) were amplified by PCR from a preparation of the EAF plasmid of EPEC strain B171 and cloned into plasmid vectors. An intact copy of bfpA was subcloned into the heat inducible prokaryotic expression vector pCYTEXP1, and the resulting pBfpA was used to transform the aroA S. typhimurium strain SL3261, generating SL3261(pBfpA). The recombinant vaccine strain was able to express, but not to process, rBfpA as evidenced by a prominent 21 kDa protein that crossreacted with anti-BFP antiserum found only in extracts of heat-treated SL3261(pBfpA), but not in strains of untreated SL3261(pBfpA) or SL3261 not carrying the plasmid. Furthermore, rBfpA accumulation was not toxic to the Salmonella host, as evidenced by similar plating efficiencies between induced and uninduced strains of SL3261(pBfpA). Finally, SL3261(pBfpA) orally administered to BALB/c mice was capable of eliciting a sustained and vigorous humoral immune response to BfpA, achievable even with a single oral dose of approximately 10(9) organisms. Therefore, this pilin product may serve as a potential immunogen as part of a live combined vaccine strategy to prevent two of the major public health problems in Brazil--salmonellosis and EPEC childhood diahrrea.EPECBFPVaccineOralProteínas da Membrana Bacteriana Externa/genéticaVacinas Bacterianas/imunologiaProteínas de Escherichia coliEscherichia coli/genéticaEscherichia coli/imunologiaFímbrias Bacterianas/imunologiaProteínas de Membrana/genéticaVacinas contra SalmonellaSalmonella typhimurium/imunologiaVacinas Tíficas-ParatíficasAdministração OralAnimaisAnticorpos Antibacterianos/biossínteseEspecificidade de AnticorposProteínas da Membrana BacterianaExterna/biossínteseVacinas Bacterianas/genéticaClonagem MolecularEscherichia coli/patogenicidadeFemininoProteínas de FímbriasFímbrias Bacterianas/genéticaRegulação Bacteriana da ExpressãoGênica/imunologiaVetores Genéticos/imunologiaProteínas de Membrana/imunologiaCamundongos Endogâmicos BALB CSalmonella typhimurium/genéticaTransformação BacterianaVacinas Atenuadas/genéticaVacinas Atenuadas/imunologiaVacinas Sintéticas/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSchriefer A Expression of a pilin subunit....pdfSchriefer A Expression of a pilin subunit....pdfapplication/pdf241336https://www.arca.fiocruz.br/bitstream/icict/5652/1/Schriefer%20A%20Expression%20of%20a%20pilin%20subunit....pdf4f92cde3e00b9144e1a94836577ce16eMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
title Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
spellingShingle Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
Schriefer, Albert
EPEC
BFP
Vaccine
Oral
Proteínas da Membrana Bacteriana Externa/genética
Vacinas Bacterianas/imunologia
Proteínas de Escherichia coli
Escherichia coli/genética
Escherichia coli/imunologia
Fímbrias Bacterianas/imunologia
Proteínas de Membrana/genética
Vacinas contra Salmonella
Salmonella typhimurium/imunologia
Vacinas Tíficas-Paratíficas
Administração Oral
Animais
Anticorpos Antibacterianos/biossíntese
Especificidade de Anticorpos
Proteínas da Membrana Bacteriana
Externa/biossíntese
Vacinas Bacterianas/genética
Clonagem Molecular
Escherichia coli/patogenicidade
Feminino
Proteínas de Fímbrias
Fímbrias Bacterianas/genética
Regulação Bacteriana da Expressão
Gênica/imunologia
Vetores Genéticos/imunologia
Proteínas de Membrana/imunologia
Camundongos Endogâmicos BALB C
Salmonella typhimurium/genética
Transformação Bacteriana
Vacinas Atenuadas/genética
Vacinas Atenuadas/imunologia
Vacinas Sintéticas/imunologia
title_short Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
title_full Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
title_fullStr Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
title_full_unstemmed Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
title_sort Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
author Schriefer, Albert
author_facet Schriefer, Albert
Maltez, J. R
Silva, N
Stoeckle, M.Y
Barral Netto, Manoel
Riley, Lee Woodland
author_role author
author2 Maltez, J. R
Silva, N
Stoeckle, M.Y
Barral Netto, Manoel
Riley, Lee Woodland
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Schriefer, Albert
Maltez, J. R
Silva, N
Stoeckle, M.Y
Barral Netto, Manoel
Riley, Lee Woodland
dc.subject.en.pt_BR.fl_str_mv EPEC
BFP
Vaccine
Oral
topic EPEC
BFP
Vaccine
Oral
Proteínas da Membrana Bacteriana Externa/genética
Vacinas Bacterianas/imunologia
Proteínas de Escherichia coli
Escherichia coli/genética
Escherichia coli/imunologia
Fímbrias Bacterianas/imunologia
Proteínas de Membrana/genética
Vacinas contra Salmonella
Salmonella typhimurium/imunologia
Vacinas Tíficas-Paratíficas
Administração Oral
Animais
Anticorpos Antibacterianos/biossíntese
Especificidade de Anticorpos
Proteínas da Membrana Bacteriana
Externa/biossíntese
Vacinas Bacterianas/genética
Clonagem Molecular
Escherichia coli/patogenicidade
Feminino
Proteínas de Fímbrias
Fímbrias Bacterianas/genética
Regulação Bacteriana da Expressão
Gênica/imunologia
Vetores Genéticos/imunologia
Proteínas de Membrana/imunologia
Camundongos Endogâmicos BALB C
Salmonella typhimurium/genética
Transformação Bacteriana
Vacinas Atenuadas/genética
Vacinas Atenuadas/imunologia
Vacinas Sintéticas/imunologia
dc.subject.decs.pt_BR.fl_str_mv Proteínas da Membrana Bacteriana Externa/genética
Vacinas Bacterianas/imunologia
Proteínas de Escherichia coli
Escherichia coli/genética
Escherichia coli/imunologia
Fímbrias Bacterianas/imunologia
Proteínas de Membrana/genética
Vacinas contra Salmonella
Salmonella typhimurium/imunologia
Vacinas Tíficas-Paratíficas
Administração Oral
Animais
Anticorpos Antibacterianos/biossíntese
Especificidade de Anticorpos
Proteínas da Membrana Bacteriana
Externa/biossíntese
Vacinas Bacterianas/genética
Clonagem Molecular
Escherichia coli/patogenicidade
Feminino
Proteínas de Fímbrias
Fímbrias Bacterianas/genética
Regulação Bacteriana da Expressão
Gênica/imunologia
Vetores Genéticos/imunologia
Proteínas de Membrana/imunologia
Camundongos Endogâmicos BALB C
Salmonella typhimurium/genética
Transformação Bacteriana
Vacinas Atenuadas/genética
Vacinas Atenuadas/imunologia
Vacinas Sintéticas/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2012-10-05T18:51:53Z
dc.date.available.fl_str_mv 2012-10-05T18:51:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHRIEFER, A. et al. Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. Vaccine, v. 17, n. 7-8, p. 770-8, feb. 1999.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5652
dc.identifier.issn.none.fl_str_mv 0264-410X
identifier_str_mv SCHRIEFER, A. et al. Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. Vaccine, v. 17, n. 7-8, p. 770-8, feb. 1999.
0264-410X
url https://www.arca.fiocruz.br/handle/icict/5652
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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