Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46046 |
Resumo: | Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. |
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Nascimento, Ana Paula Barbosa doMedeiros Filho, FernandoPauer, HeidiAntunes, Luis Caetano MarthaSousa, HérioSenger, HermesAlbano, Rodolpho MattosSantos, Marcelo Trindade dosCarvalho-Assef, Ana Paula D’AlincourtSilva, Fabrício Alves Barbosa da2021-02-11T19:05:10Z2021-02-11T19:05:10Z2020NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020.2045-2322https://www.arca.fiocruz.br/handle/icict/4604610.1038/s41598-020-69944-6engNature ResearchPseudomonas aeruginosaSPM-1Produtoras de metalo-beta-lactamaseGenômica comparativaAnálise fenotípicaSPM‑1Metallo‑beta‑lactamase‑producingPseudomonas aeruginosaComparative genomicsPhenotypic analysisCharacterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz; Escola Nacional de Saúde Pùblica Sergio Arouca. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil.Universidade Federal de São Carlos. Departamento de Computação. São Carlos, SP, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections. The Brazilian isolate, named CCBH4851, is a multidrug-resistant clone belonging to the sequence type 277. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated with the presence of the blaSPM-1 gene, encoding a metallo-beta-lactamase, in combination with other exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are essential to identify key features of their physiology that may lead to the identification of new targets for therapy. Using both Illumina and PacBio sequencing data, we obtained a single contig representing the CCBH4851 genome with annotated features that were consistent with data reported for the species. However, comparative analysis with other Pseudomonas aeruginosa strains revealed genomic differences regarding virulence factors and regulatory proteins. In addition, we performed phenotypic assays that revealed CCBH4851 is impaired in bacterial motilities and biofilm formation. On the other hand, CCBH4851 genome contained acquired genomic islands that carry transcriptional factors, virulence and antimicrobial resistance-related genes. Presence of single nucleotide polymorphisms in the core genome, mainly those located in resistance-associated genes, suggests that these mutations may also influence the multidrug-resistant behavior of CCBH4851. Overall, characterization of Pseudomonas aeruginosa CCBH4851 complete genome revealed the presence of features that strongly relates to the virulence and antibiotic resistance profile of this important infectious agent.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46046/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnaPaulaAlincourtAssef_etal_IOC_2020.pdfAnaPaulaAlincourtAssef_etal_IOC_2020.pdfapplication/pdf2224550https://www.arca.fiocruz.br/bitstream/icict/46046/2/AnaPaulaAlincourtAssef_etal_IOC_2020.pdf75e5933a5ff031d3d8b6f66075858e65MD52TEXTAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.txtAnaPaulaAlincourtAssef_etal_IOC_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| title |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| spellingShingle |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis Nascimento, Ana Paula Barbosa do Pseudomonas aeruginosa SPM-1 Produtoras de metalo-beta-lactamase Genômica comparativa Análise fenotípica SPM‑1 Metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa Comparative genomics Phenotypic analysis |
| title_short |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| title_full |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| title_fullStr |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| title_full_unstemmed |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| title_sort |
Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis |
| author |
Nascimento, Ana Paula Barbosa do |
| author_facet |
Nascimento, Ana Paula Barbosa do Medeiros Filho, Fernando Pauer, Heidi Antunes, Luis Caetano Martha Sousa, Hério Senger, Hermes Albano, Rodolpho Mattos Santos, Marcelo Trindade dos Carvalho-Assef, Ana Paula D’Alincourt Silva, Fabrício Alves Barbosa da |
| author_role |
author |
| author2 |
Medeiros Filho, Fernando Pauer, Heidi Antunes, Luis Caetano Martha Sousa, Hério Senger, Hermes Albano, Rodolpho Mattos Santos, Marcelo Trindade dos Carvalho-Assef, Ana Paula D’Alincourt Silva, Fabrício Alves Barbosa da |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nascimento, Ana Paula Barbosa do Medeiros Filho, Fernando Pauer, Heidi Antunes, Luis Caetano Martha Sousa, Hério Senger, Hermes Albano, Rodolpho Mattos Santos, Marcelo Trindade dos Carvalho-Assef, Ana Paula D’Alincourt Silva, Fabrício Alves Barbosa da |
| dc.subject.other.pt_BR.fl_str_mv |
Pseudomonas aeruginosa SPM-1 Produtoras de metalo-beta-lactamase Genômica comparativa Análise fenotípica |
| topic |
Pseudomonas aeruginosa SPM-1 Produtoras de metalo-beta-lactamase Genômica comparativa Análise fenotípica SPM‑1 Metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa Comparative genomics Phenotypic analysis |
| dc.subject.en.pt_BR.fl_str_mv |
SPM‑1 Metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa Comparative genomics Phenotypic analysis |
| description |
Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020 |
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2021-02-11T19:05:10Z |
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2021-02-11T19:05:10Z |
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info:eu-repo/semantics/article |
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article |
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NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020. |
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https://www.arca.fiocruz.br/handle/icict/46046 |
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2045-2322 |
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10.1038/s41598-020-69944-6 |
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NASCIMENTO, Ana Paula Barbosa do et al. Characterization of a SPM‑1 metallo‑beta‑lactamase‑producing Pseudomonas aeruginosa by comparative genomics and phenotypic analysis. Scientific Reports, v. 20, 13192, 16p, 2020. 2045-2322 10.1038/s41598-020-69944-6 |
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