Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites

Detalhes bibliográficos
Autor(a) principal: Mkada-Driss, Imen
Data de Publicação: 2014
Outros Autores: Lahmadi, Ramzi, Chakroun, Ahmed S, Talbi, Chiraz, Guerbouj, Souheila, Driss, Mehdi, Elamine, Elwaleed M, Cupolillo, Elisa, Mhkhtar, Moawia M, Guizani, Ikram
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10695
Resumo: Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.
id CRUZ_403692a67a621445d46b4cab5cd73301
oai_identifier_str oai:www.arca.fiocruz.br:icict/10695
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Mkada-Driss, ImenLahmadi, RamziChakroun, Ahmed STalbi, ChirazGuerbouj, SouheilaDriss, MehdiElamine, Elwaleed MCupolillo, ElisaMhkhtar, Moawia MGuizani, Ikram2015-06-08T14:01:51Z2015-06-08T14:01:51Z2014MKAda-DRISS, Imen et al. Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites. Plos One, v.9, n.10, 13p, oct. 2014.https://www.arca.fiocruz.br/handle/icict/1069510.1371/journal.pone.0109773engPlos OneScreening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversité Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.University of Khartoum. Institute of Endemic Diseases. Khartoum, Sudan.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.University of Khartoum. Institute of Endemic Diseases. Khartoum, Sudan.Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.Visceral leishmaniasis (VL) is mainly due to the Leishmania donovani complex. VL is endemic in many countries worldwide including East Africa and the Mediterranean region where the epidemiology is complex. Taxonomy of these pathogens is under controversy but there is a correlation between their genetic diversity and geographical origin. With steady increase in genome knowledge, RAPD is still a useful approach to identify and characterize novel DNA markers. Our aim was to identify and characterize polymorphic DNA markers in VL Leishmania parasites in diverse geographic regions using RAPD in order to constitute a pool of PCR targets having the potential to differentiate among the VL parasites. 100 different oligonucleotide decamers having arbitrary DNA sequences were screened for reproducible amplification and a selection of 28 was used to amplify DNA from 12 L. donovani, L. archibaldi and L. infantum strains having diverse origins. A total of 155 bands were amplified of which 60.65% appeared polymorphic. 7 out of 28 primers provided monomorphic patterns. Phenetic analysis allowed clustering the parasites according to their geographical origin. Differentially amplified bands were selected, among them 22 RAPD products were successfully cloned and sequenced. Bioinformatic analysis allowed mapping of the markers and sequences and priming sites analysis. This study was complemented with Southern-blot to confirm assignment of markers to the kDNA. The bioinformatic analysis identified 16 nuclear and 3 minicircle markers. Analysis of these markers highlighted polymorphisms at RAPD priming sites with mainly 59 end transversions, and presence of inter– and intra– taxonomic complex sequence and microsatellites variations; a bias in transitions over transversions and indels between the different sequences compared is observed, which is however less marked between L. infantum and L. donovani. The study delivers a pool of well-documented polymorphic DNA markers, to develop molecular diagnostics assays to characterize and differentiate VL causing agents.LeishmaniaVisceral leishmaniasisLeishmania ParasitesDNA MarkersLeishmaniose VisceralMarcadores GenéticosParasitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10695/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALelisa_cupolilloetal_IOC_2014.pdfapplication/pdf593266https://www.arca.fiocruz.br/bitstream/icict/10695/2/elisa_cupolilloetal_IOC_2014.pdf592360e398195378af371a01766d83f4MD52TEXTelisa_cupolilloetal_IOC_2014.pdf.txtelisa_cupolilloetal_IOC_2014.pdf.txtExtracted texttext/plain72382https://www.arca.fiocruz.br/bitstream/icict/10695/3/elisa_cupolilloetal_IOC_2014.pdf.txt14d1c6999055164af8ade6550e4025c5MD53icict/106952022-06-24 13:08:46.756oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
title Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
spellingShingle Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
Mkada-Driss, Imen
Leishmania
Visceral leishmaniasis
Leishmania Parasites
DNA Markers
Leishmaniose Visceral
Marcadores Genéticos
Parasitos
title_short Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
title_full Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
title_fullStr Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
title_full_unstemmed Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
title_sort Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites
author Mkada-Driss, Imen
author_facet Mkada-Driss, Imen
Lahmadi, Ramzi
Chakroun, Ahmed S
Talbi, Chiraz
Guerbouj, Souheila
Driss, Mehdi
Elamine, Elwaleed M
Cupolillo, Elisa
Mhkhtar, Moawia M
Guizani, Ikram
author_role author
author2 Lahmadi, Ramzi
Chakroun, Ahmed S
Talbi, Chiraz
Guerbouj, Souheila
Driss, Mehdi
Elamine, Elwaleed M
Cupolillo, Elisa
Mhkhtar, Moawia M
Guizani, Ikram
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mkada-Driss, Imen
Lahmadi, Ramzi
Chakroun, Ahmed S
Talbi, Chiraz
Guerbouj, Souheila
Driss, Mehdi
Elamine, Elwaleed M
Cupolillo, Elisa
Mhkhtar, Moawia M
Guizani, Ikram
dc.subject.en.pt_BR.fl_str_mv Leishmania
Visceral leishmaniasis
Leishmania Parasites
DNA Markers
topic Leishmania
Visceral leishmaniasis
Leishmania Parasites
DNA Markers
Leishmaniose Visceral
Marcadores Genéticos
Parasitos
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Visceral
Marcadores Genéticos
Parasitos
description Université Tunis el Manar. Institut Pasteur de Tunis. Laboratoire d’Epide´miologie Mole´culaire et de Pathologie Expe´rimentale (LR11IPT04)/Laboratoire d’Epide´miologie et d’Ecologie Parasitaire (LR00SP04). Tunis, Tunisia.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:51Z
dc.date.available.fl_str_mv 2015-06-08T14:01:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MKAda-DRISS, Imen et al. Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites. Plos One, v.9, n.10, 13p, oct. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10695
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0109773
identifier_str_mv MKAda-DRISS, Imen et al. Screening and Characterization of RAPD Markers in Viscerotropic Leishmania Parasites. Plos One, v.9, n.10, 13p, oct. 2014.
10.1371/journal.pone.0109773
url https://www.arca.fiocruz.br/handle/icict/10695
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10695/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10695/2/elisa_cupolilloetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10695/3/elisa_cupolilloetal_IOC_2014.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
592360e398195378af371a01766d83f4
14d1c6999055164af8ade6550e4025c5
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324735479644160

Registros relacionados