Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility

Detalhes bibliográficos
Autor(a) principal: Bonecini-Almeida, Maria da Gloria
Data de Publicação: 2000
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33610
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Hospital Evandro Chagas. Departamento de Microbiologia e Imunologia. Laboratório de Imunologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Bonecini-Almeida, Maria da Gloria2019-06-20T22:19:43Z2019-06-20T22:19:43Z2000BONECINI-ALMEIDA, Maria da Gloria et al. Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 95, v. 4, p. 491-494, 2000.0074-0276https://www.arca.fiocruz.br/handle/icict/3361010.1590/S0074-027620000004000081678-8060engInstituto Oswaldo CruzFlow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibilityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Hospital Evandro Chagas. Departamento de Microbiologia e Imunologia. Laboratório de Imunologia Celular. Rio de Janeiro, RJ, Brasil.Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations caused by different microorganisms, including Mycobacterium tuberculosis. However, this tool can be used for several others analysis. We will discuss some applications for flow cytometry to the study of M. tuberculosis, mainly on cell surface antigens, mycobacterial secreted proteins, their interaction with the immune system using inflammatory cells recovered from peripheral blood, alveolar and pleura spaces and the influence of M. tuberculosis on apoptosis, and finally the rapid determination of drug susceptibility. All of these examples highlight the usefulness of flow cytometry in the study of M. tuberculosis infection.TuberculosisMacrophagesDrug susceptibilityCytometryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33610/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Bonecini-Almeida_Maria_INI_2000.pdfve_Bonecini-Almeida_Maria_INI_2000.pdfapplication/pdf48022https://www.arca.fiocruz.br/bitstream/icict/33610/2/ve_Bonecini-Almeida_Maria_INI_2000.pdf32ff1b15ec3989ef7e414ab5c4e7f013MD52TEXTve_Bonecini-Almeida_Maria_INI_2000.pdf.txtve_Bonecini-Almeida_Maria_INI_2000.pdf.txtExtracted texttext/plain20960https://www.arca.fiocruz.br/bitstream/icict/33610/3/ve_Bonecini-Almeida_Maria_INI_2000.pdf.txt9fb84244dce4f8c3b6514d3d97a5ca95MD53icict/336102019-06-21 02:00:21.604oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-21T05:00:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
title Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
spellingShingle Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
Bonecini-Almeida, Maria da Gloria
Tuberculosis
Macrophages
Drug susceptibility
Cytometry
title_short Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
title_full Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
title_fullStr Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
title_full_unstemmed Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
title_sort Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
author Bonecini-Almeida, Maria da Gloria
author_facet Bonecini-Almeida, Maria da Gloria
author_role author
dc.contributor.author.fl_str_mv Bonecini-Almeida, Maria da Gloria
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
Macrophages
Drug susceptibility
Cytometry
topic Tuberculosis
Macrophages
Drug susceptibility
Cytometry
description Fundação Oswaldo Cruz. Centro de Pesquisa Hospital Evandro Chagas. Departamento de Microbiologia e Imunologia. Laboratório de Imunologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2019-06-20T22:19:43Z
dc.date.available.fl_str_mv 2019-06-20T22:19:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BONECINI-ALMEIDA, Maria da Gloria et al. Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 95, v. 4, p. 491-494, 2000.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33610
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762000000400008
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BONECINI-ALMEIDA, Maria da Gloria et al. Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 95, v. 4, p. 491-494, 2000.
0074-0276
10.1590/S0074-02762000000400008
1678-8060
url https://www.arca.fiocruz.br/handle/icict/33610
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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