A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV

Detalhes bibliográficos
Autor(a) principal: Kulkarni, Vandana
Data de Publicação: 2021
Outros Autores: Queiroz, Artur Trancoso Lopo de, Sangle, Shashi, Kagal, Anju, Salvi, Sonali, Gupta, Amita, Ellner, Jerrold, Kadam, Dileep, Rolla, Valeria C., Andrade, Bruno de Bezerril, Salgame, Padmini, Mave, Vidya
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46533
Resumo: CRDF Global (Award number DAA3-17-63158-1/Country: India). Research data in this manuscript were collected, in part, as part of the Regional Prospective Observational Research for Tuberculosis (RePORT) India Consortium. This work was funded in whole or in part by federal funds from the Government of India Department of Biotechnology (DBT); the Indian Council of Medical Research (ICMR); the National Institutes of Health National Institute of Allergy and Infectious Diseases Office of AIDS Research; CRDF Global; the National Institutes of Health Baltimore-Washington-India Clinical Trials Unit for National Institute of Allergy and Infectious Diseases Networks (UM1AI069465 to VM and AG); and the National Institutes of Health (R01A1I097494 to VM and AG). The work of BBA was supported by the Intramural Research Program of the Oswaldo Cruz Foundation, and the National Council for Scientific and Technological Development (senior CNPq fellowship—tier 1D), Brazil.
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spelling Kulkarni, VandanaQueiroz, Artur Trancoso Lopo deSangle, ShashiKagal, AnjuSalvi, SonaliGupta, AmitaEllner, JerroldKadam, DileepRolla, Valeria C.Andrade, Bruno de BezerrilSalgame, PadminiMave, Vidya2021-04-07T11:48:44Z2021-04-07T11:48:44Z2021KULKARNI, Vandana et al. A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV. Frontiers in Immunology, v. 12, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/4653310.3389/fimmu.2021.631165CRDF Global (Award number DAA3-17-63158-1/Country: India). Research data in this manuscript were collected, in part, as part of the Regional Prospective Observational Research for Tuberculosis (RePORT) India Consortium. This work was funded in whole or in part by federal funds from the Government of India Department of Biotechnology (DBT); the Indian Council of Medical Research (ICMR); the National Institutes of Health National Institute of Allergy and Infectious Diseases Office of AIDS Research; CRDF Global; the National Institutes of Health Baltimore-Washington-India Clinical Trials Unit for National Institute of Allergy and Infectious Diseases Networks (UM1AI069465 to VM and AG); and the National Institutes of Health (R01A1I097494 to VM and AG). The work of BBA was supported by the Intramural Research Program of the Oswaldo Cruz Foundation, and the National Council for Scientific and Technological Development (senior CNPq fellowship—tier 1D), Brazil.Johns Hopkins University Clinical Research Site. Byramjee-Jeejeebhoy Government Medical College. Pune, India.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative, Salvador, BA, Brazil.Johns Hopkins University Clinical Research Site. Byramjee-Jeejeebhoy Government Medical College. Pune, India.Johns Hopkins University Clinical Research Site. Byramjee-Jeejeebhoy Government Medical College. Pune, India.Johns Hopkins University Clinical Research Site. Byramjee-Jeejeebhoy Government Medical College. Pune, India.Johns Hopkins University School of Medicine. Baltimore, MD, United States.Rutgers- New Jersey Medical School. Center for Emerging Pathogens. Newark, NJ, United States.Johns Hopkins University Clinical Research Site. Byramjee-Jeejeebhoy Government Medical College. Pune, India.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative, Salvador, BA, Brazil.Rutgers- New Jersey Medical School. Center for Emerging Pathogens. Newark, NJ, United States.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Johns Hopkins University School of Medicine. Baltimore, MD, United States.Transcriptomic signatures for tuberculosis (TB) have been proposed and represent a promising diagnostic tool. Data remain limited in persons with advanced HIV. Methods: We enrolled 30 patients with advanced HIV (CD4 < 100 cells/mm3) in India; 16 with active TB and 14 without. Whole-blood RNA sequencing was performed; these data were merged with a publicly available dataset from Uganda (n = 33; 18 with TB and 15 without). Transcriptomic profiling and machine learning algorithms identified an optimal gene signature for TB classification. Receiver operating characteristic analysis was used to assess performance. Results: Among 565 differentially expressed genes identified for TB, 40 were shared across India and Uganda cohorts. Common upregulated pathways reflect Toll-like receptor cascades and neutrophil degranulation. The machine-learning decision-tree algorithm selected gene expression values from RAB20 and INSL3 as most informative for TB classification. The signature accurately classified TB in discovery cohorts (India AUC 0.95 and Uganda AUC 1.0; p < 0.001); accuracy was fair in external validation cohorts. Conclusions: Expression values of RAB20 and INSL3 genes in peripheral blood compose a biosignature that accurately classified TB status among patients with advanced HIV in two geographically distinct cohorts. The functional analysis suggests pathways previously reported in TB pathogenesis.engFrontiers MediaHIVTuberculoseDiagnósticoGenesHIVTuberculosisTtranscriptomicsDiagnosisGene signatureA Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIVinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/46533/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALKulkarni, Vandara A two-gene....pdfKulkarni, Vandara A two-gene....pdfapplication/pdf3717604https://www.arca.fiocruz.br/bitstream/icict/46533/2/Kulkarni%2c%20Vandara%20A%20two-gene....pdfc34fb61108af1fe549a1b8d9b4f1e7faMD52TEXTKulkarni, Vandara A two-gene....pdf.txtKulkarni, Vandara A two-gene....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
title A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
spellingShingle A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
Kulkarni, Vandana
HIV
Tuberculose
Diagnóstico
Genes
HIV
Tuberculosis
Ttranscriptomics
Diagnosis
Gene signature
title_short A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
title_full A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
title_fullStr A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
title_full_unstemmed A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
title_sort A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV
author Kulkarni, Vandana
author_facet Kulkarni, Vandana
Queiroz, Artur Trancoso Lopo de
Sangle, Shashi
Kagal, Anju
Salvi, Sonali
Gupta, Amita
Ellner, Jerrold
Kadam, Dileep
Rolla, Valeria C.
Andrade, Bruno de Bezerril
Salgame, Padmini
Mave, Vidya
author_role author
author2 Queiroz, Artur Trancoso Lopo de
Sangle, Shashi
Kagal, Anju
Salvi, Sonali
Gupta, Amita
Ellner, Jerrold
Kadam, Dileep
Rolla, Valeria C.
Andrade, Bruno de Bezerril
Salgame, Padmini
Mave, Vidya
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kulkarni, Vandana
Queiroz, Artur Trancoso Lopo de
Sangle, Shashi
Kagal, Anju
Salvi, Sonali
Gupta, Amita
Ellner, Jerrold
Kadam, Dileep
Rolla, Valeria C.
Andrade, Bruno de Bezerril
Salgame, Padmini
Mave, Vidya
dc.subject.other.pt_BR.fl_str_mv HIV
Tuberculose
Diagnóstico
Genes
topic HIV
Tuberculose
Diagnóstico
Genes
HIV
Tuberculosis
Ttranscriptomics
Diagnosis
Gene signature
dc.subject.en.pt_BR.fl_str_mv HIV
Tuberculosis
Ttranscriptomics
Diagnosis
Gene signature
description CRDF Global (Award number DAA3-17-63158-1/Country: India). Research data in this manuscript were collected, in part, as part of the Regional Prospective Observational Research for Tuberculosis (RePORT) India Consortium. This work was funded in whole or in part by federal funds from the Government of India Department of Biotechnology (DBT); the Indian Council of Medical Research (ICMR); the National Institutes of Health National Institute of Allergy and Infectious Diseases Office of AIDS Research; CRDF Global; the National Institutes of Health Baltimore-Washington-India Clinical Trials Unit for National Institute of Allergy and Infectious Diseases Networks (UM1AI069465 to VM and AG); and the National Institutes of Health (R01A1I097494 to VM and AG). The work of BBA was supported by the Intramural Research Program of the Oswaldo Cruz Foundation, and the National Council for Scientific and Technological Development (senior CNPq fellowship—tier 1D), Brazil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-04-07T11:48:44Z
dc.date.available.fl_str_mv 2021-04-07T11:48:44Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KULKARNI, Vandana et al. A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV. Frontiers in Immunology, v. 12, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46533
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2021.631165
identifier_str_mv KULKARNI, Vandana et al. A Two-Gene Signature for Tuberculosis Diagnosis in Persons With Advanced HIV. Frontiers in Immunology, v. 12, 2021.
1664-3224
10.3389/fimmu.2021.631165
url https://www.arca.fiocruz.br/handle/icict/46533
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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