Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.

Detalhes bibliográficos
Autor(a) principal: Dorneles, Elaine Maria Seles
Data de Publicação: 2015
Outros Autores: Lima, Graciela Kunrath, Carvalho, Andréa Teixeira de, Araújo, Márcio Sobreira Silva, Martins Filho, Olindo Assis, Sriranganathan, Nammalwar, Qublan, Hamzeh Al, BryanHeinemann, Marcos, Lage, Andrey Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13070
Resumo: Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil
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spelling Dorneles, Elaine Maria SelesLima, Graciela KunrathCarvalho, Andréa Teixeira deAraújo, Márcio Sobreira SilvaMartins Filho, Olindo AssisSriranganathan, NammalwarQublan, Hamzeh AlBryanHeinemann, MarcosLage, Andrey Pereira2016-03-10T12:20:40Z2016-03-10T12:20:40Z2015DORNELES, Elaine Maria Seles et al. Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51. PLoS One, vol. 10, n. 9, art. e0136696, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/130700.1371/journal.pone.0136696engPublic Library of ScienceImmune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilVirginia Polytechnic Institute and State University. Virginia Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology. Center for Molecular Medicine and Infectious Diseases. Blacksburg, Virginia, USAVirginia Polytechnic Institute and State University. Virginia Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology. Center for Molecular Medicine and Infectious Diseases. Blacksburg, Virginia, USAUniversidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal. São Paulo, SP, BrazilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilBrucella abortus S19 and RB51 strains have been successfully used to control bovine brucellosis worldwide; however, currently, most of our understanding of the protective immune response induced by vaccination comes from studies in mice. The aim of this study was to characterize and compare the immune responses induced in cattle prime-immunized with B. abortus S19 or RB51 and revaccinated with RB51. Female calves, aged 4 to 8 months, were vaccinated with either vaccine S19 (0.6–1.2 x 1011 CFU) or RB51 (1.3 x 1010 CFU) on day 0, and revaccinated with RB51 (1.3 x 1010 CFU) on day 365 of the experiment. Characterization of the immune response was performed using serum and peripheral blood mononuclear cells. Blood samples were collected on days 0, 28, 210, 365, 393 and 575 post-immunization. Results showed that S19 and RB51 vaccination induced an immune response characterized by proliferation of CD4+ and CD8+ T-cells; IFN-ɣ and IL-17A production by CD4+ T-cells; cytotoxic CD8+ T-cells; IL-6 secretion; CD4+ and CD8+ memory cells; antibodies of IgG1 class; and expression of the phenotypes of activation in T-cells. However, the immune response stimulated by S19 compared to RB51 showed higher persistency of IFN-ɣ and CD4+ memory cells, induction of CD21+ memory cells and higher secretion of IL-6. After RB51 revaccination, the immune response was chiefly characterized by increase in IFN-ɣ expression, proliferation of antigen-specific CD4+ and CD8+ T-cells, cytotoxic CD8+ T-cells and decrease of IL-6 production in both groups. Nevertheless, a different polarization of the immune response, CD4+- or CD8+-dominant, was observed after the booster with RB51 for S19 and RB51 prime-vaccinated animals, respectively. Our results indicate that after prime vaccination both vaccine strains induce a strong and complex Th1 immune response, although after RB51 revaccination the differences between immune profiles induced by prime-vaccination become accentuated.Vaccination and immunizationT cellsCytotoxic T cellsImmune responseCattleVaccinesBrucellosisCytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13070/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALImmune Response of Calves Vaccinated with.pdfImmune Response of Calves Vaccinated with.pdfapplication/pdf1412040https://www.arca.fiocruz.br/bitstream/icict/13070/2/Immune%20Response%20of%20Calves%20Vaccinated%20with.pdffa5c435ab771a8ffc8fb6740cce1e706MD52TEXTImmune Response of Calves Vaccinated with.pdf.txtImmune Response of Calves Vaccinated 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dc.title.pt_BR.fl_str_mv Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
title Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
spellingShingle Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
Dorneles, Elaine Maria Seles
Vaccination and immunization
T cells
Cytotoxic T cells
Immune response
Cattle
Vaccines
Brucellosis
Cytokines
title_short Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
title_full Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
title_fullStr Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
title_full_unstemmed Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
title_sort Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
author Dorneles, Elaine Maria Seles
author_facet Dorneles, Elaine Maria Seles
Lima, Graciela Kunrath
Carvalho, Andréa Teixeira de
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Sriranganathan, Nammalwar
Qublan, Hamzeh Al
BryanHeinemann, Marcos
Lage, Andrey Pereira
author_role author
author2 Lima, Graciela Kunrath
Carvalho, Andréa Teixeira de
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Sriranganathan, Nammalwar
Qublan, Hamzeh Al
BryanHeinemann, Marcos
Lage, Andrey Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dorneles, Elaine Maria Seles
Lima, Graciela Kunrath
Carvalho, Andréa Teixeira de
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Sriranganathan, Nammalwar
Qublan, Hamzeh Al
BryanHeinemann, Marcos
Lage, Andrey Pereira
dc.subject.en.pt_BR.fl_str_mv Vaccination and immunization
T cells
Cytotoxic T cells
Immune response
Cattle
Vaccines
Brucellosis
Cytokines
topic Vaccination and immunization
T cells
Cytotoxic T cells
Immune response
Cattle
Vaccines
Brucellosis
Cytokines
description Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-10T12:20:40Z
dc.date.available.fl_str_mv 2016-03-10T12:20:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DORNELES, Elaine Maria Seles et al. Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51. PLoS One, vol. 10, n. 9, art. e0136696, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13070
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 0.1371/journal.pone.0136696
identifier_str_mv DORNELES, Elaine Maria Seles et al. Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51. PLoS One, vol. 10, n. 9, art. e0136696, 2015.
1932-6203
0.1371/journal.pone.0136696
url https://www.arca.fiocruz.br/handle/icict/13070
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library of Science
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