Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants

Detalhes bibliográficos
Autor(a) principal: Dorneles, Elaine Maria Seles
Data de Publicação: 2015
Outros Autores: Araújo, Márcio Sobreira Silva, Carvalho, Andrea Teixeira, Martins Filho, Olindo Assis, Lage, Andrey Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12566
Resumo: Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.
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spelling Dorneles, Elaine Maria SelesAraújo, Márcio Sobreira SilvaCarvalho, Andrea TeixeiraMartins Filho, Olindo AssisLage, Andrey Pereira2016-01-20T13:21:14Z2016-01-20T13:21:14Z2015DORNELES, Elaine Maria Seles et al. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants. Genet Mol Res., vol. 14, n. 1, p. 940-51, 20151676-5680https://www.arca.fiocruz.br/handle/icict/1256610.4238/2015.February.3.1engFUNPECCross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilEleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-α, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested. Anti-human IL-5 mAb showed a strong cross-reactivity with cattle and goat IL-5, while anti-human TNF-α mAb showed a selective cross-reactivity with goat TNF-α. No cross-reactivity with the ruminant cytokines was observed for anti-human IL-12 and IL-13 mAbs or for the two anti-mouse cytokine mAbs tested. The present study demonstrated the cross-reactivity of various anti-human cytokine mAbs with cattle, sheep, and goat cytokines, increasing the range of immunological biomarkers for studies in veterinary medicine.Cytokinescross-reactivityhuman cattlegoat sheepinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12566/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCross-reactivity odomestic ruminants.pdfCross-reactivity odomestic ruminants.pdfapplication/pdf4237344https://www.arca.fiocruz.br/bitstream/icict/12566/2/Cross-reactivity%20odomestic%20ruminants.pdf6be85be8164a90f34f85e4fc9b90cea5MD52TEXTCross-reactivity odomestic ruminants.pdf.txtCross-reactivity odomestic ruminants.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
title Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
spellingShingle Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
Dorneles, Elaine Maria Seles
Cytokines
cross-reactivity
human cattle
goat sheep
title_short Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
title_full Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
title_fullStr Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
title_full_unstemmed Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
title_sort Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants
author Dorneles, Elaine Maria Seles
author_facet Dorneles, Elaine Maria Seles
Araújo, Márcio Sobreira Silva
Carvalho, Andrea Teixeira
Martins Filho, Olindo Assis
Lage, Andrey Pereira
author_role author
author2 Araújo, Márcio Sobreira Silva
Carvalho, Andrea Teixeira
Martins Filho, Olindo Assis
Lage, Andrey Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dorneles, Elaine Maria Seles
Araújo, Márcio Sobreira Silva
Carvalho, Andrea Teixeira
Martins Filho, Olindo Assis
Lage, Andrey Pereira
dc.subject.en.pt_BR.fl_str_mv Cytokines
cross-reactivity
human cattle
goat sheep
topic Cytokines
cross-reactivity
human cattle
goat sheep
description Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-20T13:21:14Z
dc.date.available.fl_str_mv 2016-01-20T13:21:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DORNELES, Elaine Maria Seles et al. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants. Genet Mol Res., vol. 14, n. 1, p. 940-51, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12566
dc.identifier.issn.none.fl_str_mv 1676-5680
dc.identifier.doi.none.fl_str_mv 10.4238/2015.February.3.1
identifier_str_mv DORNELES, Elaine Maria Seles et al. Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants. Genet Mol Res., vol. 14, n. 1, p. 940-51, 2015
1676-5680
10.4238/2015.February.3.1
url https://www.arca.fiocruz.br/handle/icict/12566
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FUNPEC
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/12566/2/Cross-reactivity%20odomestic%20ruminants.pdf
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