Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

Detalhes bibliográficos
Autor(a) principal: Espada, Caroline Ricce
Data de Publicação: 2018
Outros Autores: Ortiz, Paola Andrea, Shaw, Jeffrey Jon, Barral, Aldina Maria Prado, Costa, Jackson Mauricio Lopes, Uliana, Silvia Reni Bortolin, Coelho, Adriano Cappellazzo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28899
Resumo: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2011/20484-7 and 2015/09080-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 473343/2012-6), Brazil. SRBU is the recipient of a senior researcher scholarship from CNPq. ACC and CRE were fellows supported by FAPESP (2012/14629-5 and 2016/23405-4).
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spelling Espada, Caroline RicceOrtiz, Paola AndreaShaw, Jeffrey JonBarral, Aldina Maria PradoCosta, Jackson Mauricio LopesUliana, Silvia Reni BortolinCoelho, Adriano Cappellazzo2018-09-19T16:47:03Z2018-09-19T16:47:03Z2018ESPADA, C. R. et al. Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations. Diagnostic Microbiology and Infectious Disease, v. 91, p. 312–318, 2018.0732-8893https://www.arca.fiocruz.br/handle/icict/2889910.1016/j.diagmicrobio.2018.03.004Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2011/20484-7 and 2015/09080-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 473343/2012-6), Brazil. SRBU is the recipient of a senior researcher scholarship from CNPq. ACC and CRE were fellows supported by FAPESP (2012/14629-5 and 2016/23405-4).Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, BrasilHsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leishmania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L. (L.) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L. (Viannia) naiffi strains. The profiles of both L. (V.) shawi and L. (V.) lindenbergi were very similar to those of other L. (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil.engElsevierLeishmania (Leishmania) amazonensisEspécie Leishmania (Viannia)hsp70PCR-RFLPDiagnóstico molecularLeishmania (Leishmania) amazonensisLeishmania (Viannia) specieshsp70PCR-RFLPMolecular diagnosisIdentification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
title Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
spellingShingle Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
Espada, Caroline Ricce
Leishmania (Leishmania) amazonensis
Espécie Leishmania (Viannia)
hsp70
PCR-RFLP
Diagnóstico molecular
Leishmania (Leishmania) amazonensis
Leishmania (Viannia) species
hsp70
PCR-RFLP
Molecular diagnosis
title_short Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
title_full Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
title_fullStr Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
title_full_unstemmed Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
title_sort Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations
author Espada, Caroline Ricce
author_facet Espada, Caroline Ricce
Ortiz, Paola Andrea
Shaw, Jeffrey Jon
Barral, Aldina Maria Prado
Costa, Jackson Mauricio Lopes
Uliana, Silvia Reni Bortolin
Coelho, Adriano Cappellazzo
author_role author
author2 Ortiz, Paola Andrea
Shaw, Jeffrey Jon
Barral, Aldina Maria Prado
Costa, Jackson Mauricio Lopes
Uliana, Silvia Reni Bortolin
Coelho, Adriano Cappellazzo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Espada, Caroline Ricce
Ortiz, Paola Andrea
Shaw, Jeffrey Jon
Barral, Aldina Maria Prado
Costa, Jackson Mauricio Lopes
Uliana, Silvia Reni Bortolin
Coelho, Adriano Cappellazzo
dc.subject.other.pt_BR.fl_str_mv Leishmania (Leishmania) amazonensis
Espécie Leishmania (Viannia)
hsp70
PCR-RFLP
Diagnóstico molecular
topic Leishmania (Leishmania) amazonensis
Espécie Leishmania (Viannia)
hsp70
PCR-RFLP
Diagnóstico molecular
Leishmania (Leishmania) amazonensis
Leishmania (Viannia) species
hsp70
PCR-RFLP
Molecular diagnosis
dc.subject.en.pt_BR.fl_str_mv Leishmania (Leishmania) amazonensis
Leishmania (Viannia) species
hsp70
PCR-RFLP
Molecular diagnosis
description Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2011/20484-7 and 2015/09080-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 473343/2012-6), Brazil. SRBU is the recipient of a senior researcher scholarship from CNPq. ACC and CRE were fellows supported by FAPESP (2012/14629-5 and 2016/23405-4).
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-19T16:47:03Z
dc.date.available.fl_str_mv 2018-09-19T16:47:03Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ESPADA, C. R. et al. Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations. Diagnostic Microbiology and Infectious Disease, v. 91, p. 312–318, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28899
dc.identifier.issn.pt_BR.fl_str_mv 0732-8893
dc.identifier.doi.none.fl_str_mv 10.1016/j.diagmicrobio.2018.03.004
identifier_str_mv ESPADA, C. R. et al. Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations. Diagnostic Microbiology and Infectious Disease, v. 91, p. 312–318, 2018.
0732-8893
10.1016/j.diagmicrobio.2018.03.004
url https://www.arca.fiocruz.br/handle/icict/28899
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language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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