Extracellular vesicle-mediated RNA release in histoplasma capsulatum

Detalhes bibliográficos
Autor(a) principal: Alves, Lysangela Ronalte
Data de Publicação: 2019
Outros Autores: Silva, Roberta Peres da, Sanchez, David A., Zamith-Miranda, Daniel, Rodrigues, Marcio Lourenço, Goldenberg, Samuel, Puccia, Rosana, Nosanchuk, Joshua D.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34174
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Alves, Lysangela RonalteSilva, Roberta Peres daSanchez, David A.Zamith-Miranda, DanielRodrigues, Marcio LourençoGoldenberg, SamuelPuccia, RosanaNosanchuk, Joshua D.2019-07-16T19:18:22Z2019-07-16T19:18:22Z2019ALVES, Lysangela Ronalte et al. Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum. mSphere, v. 4, n. 2, p. 1-17, 2019.2379-5042https://www.arca.fiocruz.br/handle/icict/3417410.1128/mSphere.00176-192379-5042porAmerican Society For MicrobiologyCapsulatumExtracellular VesiclesEukaryotic CellsARNCélulas EucariotasHistoplasmaRNAVesículas ExtracelularesCélulas EucarióticasExtracellular vesicle-mediated RNA release in histoplasma capsulatuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia da Escola Paulista de Medicina. São Paulo, SP, Brasil.Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA.Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia da Escola Paulista de Medicina. São Paulo, SP, Brasil.Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA.Eukaryotic cells, including fungi, release extracellular vesicles (EVs). These lipid bilayered compartments play essential roles in cellular communication and pathogenesis. EV composition is complex and includes proteins, glycans, pigments, and RNA. RNAs with putative roles in pathogenesis have been described in EVs produced by fungi. Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen. A total of 124 mRNAs were identified in both strains. In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain. This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs. We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain. Remarkably, the most highly enriched processes were stress responses and translation. Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs. We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed. Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains. In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism. Importante information: Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis. The RNA molecules in EVs have been implicated in a variety of processes. EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available. Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection. In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum. Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/34174/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmSphere-2019-Alves.pdfmSphere-2019-Alves.pdfapplication/pdf1055453https://www.arca.fiocruz.br/bitstream/icict/34174/2/mSphere-2019-Alves.pdf049479dba230e8a592707536251c8131MD52TEXTmSphere-2019-Alves.pdf.txtmSphere-2019-Alves.pdf.txtExtracted texttext/plain64298https://www.arca.fiocruz.br/bitstream/icict/34174/3/mSphere-2019-Alves.pdf.txtb325e383f141819b3ebdbeae6db8a5a6MD53icict/341742023-07-13 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dc.title.pt_BR.fl_str_mv Extracellular vesicle-mediated RNA release in histoplasma capsulatum
title Extracellular vesicle-mediated RNA release in histoplasma capsulatum
spellingShingle Extracellular vesicle-mediated RNA release in histoplasma capsulatum
Alves, Lysangela Ronalte
Capsulatum
Extracellular Vesicles
Eukaryotic Cells
ARN
Células Eucariotas
Histoplasma
RNA
Vesículas Extracelulares
Células Eucarióticas
title_short Extracellular vesicle-mediated RNA release in histoplasma capsulatum
title_full Extracellular vesicle-mediated RNA release in histoplasma capsulatum
title_fullStr Extracellular vesicle-mediated RNA release in histoplasma capsulatum
title_full_unstemmed Extracellular vesicle-mediated RNA release in histoplasma capsulatum
title_sort Extracellular vesicle-mediated RNA release in histoplasma capsulatum
author Alves, Lysangela Ronalte
author_facet Alves, Lysangela Ronalte
Silva, Roberta Peres da
Sanchez, David A.
Zamith-Miranda, Daniel
Rodrigues, Marcio Lourenço
Goldenberg, Samuel
Puccia, Rosana
Nosanchuk, Joshua D.
author_role author
author2 Silva, Roberta Peres da
Sanchez, David A.
Zamith-Miranda, Daniel
Rodrigues, Marcio Lourenço
Goldenberg, Samuel
Puccia, Rosana
Nosanchuk, Joshua D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Lysangela Ronalte
Silva, Roberta Peres da
Sanchez, David A.
Zamith-Miranda, Daniel
Rodrigues, Marcio Lourenço
Goldenberg, Samuel
Puccia, Rosana
Nosanchuk, Joshua D.
dc.subject.other.pt_BR.fl_str_mv Capsulatum
topic Capsulatum
Extracellular Vesicles
Eukaryotic Cells
ARN
Células Eucariotas
Histoplasma
RNA
Vesículas Extracelulares
Células Eucarióticas
dc.subject.en.pt_BR.fl_str_mv Extracellular Vesicles
Eukaryotic Cells
dc.subject.es.pt_BR.fl_str_mv ARN
Células Eucariotas
dc.subject.decs.pt_BR.fl_str_mv Histoplasma
RNA
Vesículas Extracelulares
Células Eucarióticas
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-16T19:18:22Z
dc.date.available.fl_str_mv 2019-07-16T19:18:22Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Lysangela Ronalte et al. Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum. mSphere, v. 4, n. 2, p. 1-17, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34174
dc.identifier.issn.pt_BR.fl_str_mv 2379-5042
dc.identifier.doi.none.fl_str_mv 10.1128/mSphere.00176-19
dc.identifier.eissn.none.fl_str_mv 2379-5042
identifier_str_mv ALVES, Lysangela Ronalte et al. Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum. mSphere, v. 4, n. 2, p. 1-17, 2019.
2379-5042
10.1128/mSphere.00176-19
url https://www.arca.fiocruz.br/handle/icict/34174
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34174/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/34174/2/mSphere-2019-Alves.pdf
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