High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15677 |
Resumo: | This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012-00011). |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Cruz, Deu John M.Koishi, Andrea CristineTaniguchi, Juliana BossoXiaolan, LBonotto, Rafaela MilanNo, Joo HwanKim, Keum HyunBaek, SungminKim, Hee YoungWindisch, Marc PeterMosimann, Ana Luiza PamplonaBorba, Luana deLiuzzi, MichelHansen, Michael Adsetts EdbergSantos, Claudia Nunes Duarte dosFreitas-Junior, Lucio Holanda2016-09-05T17:06:37Z2016-09-05T17:06:37Z2013CRUZ, Deu John M. et al. High content screening of a kinase-focused library reveals compounds broadly-active against dengue viruses. PLOS Neglected Tropical Diseases, v. 7, n. 2, p. 1-13, 2013.1935-2727https://www.arca.fiocruz.br/handle/icict/1567710.1371/journal.pntd.0002073This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012-00011).Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Curitiba, PR, Brasil.Universidade Estadual Paulista. Araraquara, SP, Brasil.Institut Pasteur Korea. Image Mining Group.Seongnam-si, South Korea.Universidade Feevale. Novo Hamburgo, RS, Brasil.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Institut Pasteur Korea. Applied Molecular Virology. Seongnam-si, South Korea.Institut Pasteur Korea. Applied Molecular Virology. Seongnam-si, South Korea.Institut Pasteur Korea. Applied Molecular Virology. Seongnam-si, South Korea.Institut Pasteur Korea. Applied Molecular Virology. Seongnam-si, South Korea.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Institut Pasteur Korea. Early Discovery Program. Seongnam-si, South Korea.Institut Pasteur Korea. Image Mining Group. Seongnam-si, South Korea.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Dengue virus is a mosquito-borne flavivirus that has a large impact in global health. It is considered as one of the medically important arboviruses, and developing a preventive or therapeutic solution remains a top priority in the medical and scientific community. Drug discovery programs for potential dengue antivirals have increased dramatically over the last decade, largely in part to the introduction of high-throughput assays. In this study, we have developed an image-based dengue high-throughput/high-content assay (HT/HCA) using an innovative computer vision approach to screen a kinase-focused library for anti-dengue compounds. Using this dengue HT/HCA, we identified a group of compounds with a 4-(1-aminoethyl)-N-methylthiazol-2-amine as a common core structure that inhibits dengue viral infection in a human liver-derived cell line (Huh-7.5 cells). Compounds CND1201, CND1203 and CND1243 exhibited strong antiviral activities against all four dengue serotypes. Plaque reduction and time-of-addition assays suggests that these compounds interfere with the late stage of viral infection cycle. These findings demonstrate that our image-based dengue HT/HCA is a reliable tool that can be used to screen various chemical libraries for potential dengue antiviral candidates.porHigh content screening of a kinase-focused library reveals compounds broadly-active against Dengue virusesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengueFlavivirusAntiviral AgentsArbovirusesArbovirusAntiviraisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15677/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALHigh Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses.pdfHigh Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses.pdfapplication/pdf974142https://www.arca.fiocruz.br/bitstream/icict/15677/2/High%20Content%20Screening%20of%20a%20Kinase-Focused%20Library%20Reveals%20Compounds%20Broadly-Active%20against%20Dengue%20Viruses.pdfb925c87148fd63793830d2b6885d7f50MD52TEXTHigh Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses.pdf.txtHigh Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses.pdf.txtExtracted texttext/plain70063https://www.arca.fiocruz.br/bitstream/icict/15677/3/High%20Content%20Screening%20of%20a%20Kinase-Focused%20Library%20Reveals%20Compounds%20Broadly-Active%20against%20Dengue%20Viruses.pdf.txt9d0f2348528c418fab4195490f3e8e79MD53icict/156772022-10-20 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| dc.title.pt_BR.fl_str_mv |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| title |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| spellingShingle |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses Cruz, Deu John M. Dengue Flavivirus Antiviral Agents Arboviruses Arbovirus Antivirais |
| title_short |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| title_full |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| title_fullStr |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| title_full_unstemmed |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| title_sort |
High content screening of a kinase-focused library reveals compounds broadly-active against Dengue viruses |
| author |
Cruz, Deu John M. |
| author_facet |
Cruz, Deu John M. Koishi, Andrea Cristine Taniguchi, Juliana Bosso Xiaolan, L Bonotto, Rafaela Milan No, Joo Hwan Kim, Keum Hyun Baek, Sungmin Kim, Hee Young Windisch, Marc Peter Mosimann, Ana Luiza Pamplona Borba, Luana de Liuzzi, Michel Hansen, Michael Adsetts Edberg Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda |
| author_role |
author |
| author2 |
Koishi, Andrea Cristine Taniguchi, Juliana Bosso Xiaolan, L Bonotto, Rafaela Milan No, Joo Hwan Kim, Keum Hyun Baek, Sungmin Kim, Hee Young Windisch, Marc Peter Mosimann, Ana Luiza Pamplona Borba, Luana de Liuzzi, Michel Hansen, Michael Adsetts Edberg Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cruz, Deu John M. Koishi, Andrea Cristine Taniguchi, Juliana Bosso Xiaolan, L Bonotto, Rafaela Milan No, Joo Hwan Kim, Keum Hyun Baek, Sungmin Kim, Hee Young Windisch, Marc Peter Mosimann, Ana Luiza Pamplona Borba, Luana de Liuzzi, Michel Hansen, Michael Adsetts Edberg Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Flavivirus |
| topic |
Dengue Flavivirus Antiviral Agents Arboviruses Arbovirus Antivirais |
| dc.subject.en.En.fl_str_mv |
Antiviral Agents Arboviruses |
| dc.subject.decs.pt_BR.fl_str_mv |
Arbovirus Antivirais |
| description |
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012-00011). |
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2013 |
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2013 |
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2016-09-05T17:06:37Z |
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2016-09-05T17:06:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CRUZ, Deu John M. et al. High content screening of a kinase-focused library reveals compounds broadly-active against dengue viruses. PLOS Neglected Tropical Diseases, v. 7, n. 2, p. 1-13, 2013. |
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https://www.arca.fiocruz.br/handle/icict/15677 |
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1935-2727 |
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10.1371/journal.pntd.0002073 |
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CRUZ, Deu John M. et al. High content screening of a kinase-focused library reveals compounds broadly-active against dengue viruses. PLOS Neglected Tropical Diseases, v. 7, n. 2, p. 1-13, 2013. 1935-2727 10.1371/journal.pntd.0002073 |
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