Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.

Svensjö, Nils Erik; Batista, Paulo Ricardo; Brodskyn, Claudia Ida; Silva, Robson Amaro Augusto da; Lima, Ana Paula Cabral de Araujo; Pereira, Verônica Schmitz; Bou Habib, Elvira Maria Saraiva Chequer; Pesquero, João Bosco; Mori, Marcelo Alves da Silva; Müller-Esterl, Werner; Scharfstein, Julio

Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.

Detalhes bibliográficos
Autor(a) principal:	Svensjö, Nils Erik
Data de Publicação:	2006
Outros Autores:	Batista, Paulo Ricardo, Brodskyn, Claudia Ida, Silva, Robson Amaro Augusto da, Lima, Ana Paula Cabral de Araujo, Pereira, Verônica Schmitz, Bou Habib, Elvira Maria Saraiva Chequer, Pesquero, João Bosco, Mori, Marcelo Alves da Silva, Müller-Esterl, Werner, Scharfstein, Julio
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/8398
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil

Metadados do item

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network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Svensjö, Nils ErikBatista, Paulo RicardoBrodskyn, Claudia IdaSilva, Robson Amaro Augusto daLima, Ana Paula Cabral de AraujoPereira, Verônica SchmitzBou Habib, Elvira Maria Saraiva ChequerPesquero, João BoscoMori, Marcelo Alves da SilvaMüller-Esterl, WernerScharfstein, Julio2014-09-18T16:18:30Z2014-09-18T16:18:30Z2006SVENSJO, E. et al. Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex. Microbes Infection, v.8, n. 1, p. 206-220, 2006.1286-4579https://www.arca.fiocruz.br/handle/icict/839810.1016/j.micinf.2005.06.016engElsevier SASInterplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, BrasilUniversidade Federal do Estado de São Paulo. UNIFESP. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, BrasilUniversidade Federal do Estado de São Paulo. UNIFESP. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, BrasilUniversity of Frankfurt Medical School. Institute for Biochemistry II. Frankfurt, GermanyFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilKinins, the vasoactive peptides proteolytically liberated from kininogens, were recently recognized as signals alerting the innate immune system. Here we demonstrate that Leishmania donovani and Leishmania chagasi, two etiological agents of visceral leishmaniasis (VL), activate the kinin system. Intravital microscopy in the hamster cheek pouch showed that topically applied promastigotes induced macromolecular leakage (FITC-dextran) through postcapillary venules. Peaking at 15 min, the parasite-induced leakage was drastically enhanced by captopril (Cap), an inhibitor of angiotensin-converting enzyme (ACE), a kinin-degrading metallopeptidase. The enhanced microvascular responses were cancelled by HOE-140, an antagonist of the B2 bradykinin receptor (B2R), or by pre-treatment of promastigotes with the irreversible cysteine proteinase inhibitor N-methylpiperazine-urea-Phe-homoPhe-vinylsulfone-benzene (N-Pip-hF-VSPh). In agreement with the above-mentioned data, the promastigotes vigorously induced edema in the paw of Cap-treated J129 mice, but not Cap-B2R–/– mice. Analysis of parasite-induced breakdown of high molecular weight kininogens (HK), combined with active site-affinity-labeling with biotin- N-Pip-hF-VSPh, identified 35–40 kDa proteins as kinin-releasing cysteine peptidases.We then checked if macrophage infectivity was influenced by interplay between these kinin-releasing parasite proteases, kininogens, and kinin-degrading peptidases (i.e. ACE). Our studies revealed that full-fledged B2R engagement resulted in vigorous increase of L. chagasi uptake by resident macrophages. Evidence that inflammatory macrophages treated with HOE-140 became highly susceptible to amastigote outgrowth, assessed 72 h after initial macrophage interaction, further suggests that the kinin/B2R activation pathway may critically modulate inflammation and innate immunity in visceral leishmaniasis.LeishmaniasisInnate immunityInflammationMacrophagesEndotheliumKininsAngiotensin-converting enzymeCysteine proteasesPermeabilidade Capilar/fisiologiaCisteína Endopeptidases/metabolismoCininas/metabolismoLeishmania donovani/enzimologiaLeishmania infantum/enzimologiaMacrófagos/metabolismoAnimaisCricetinaeDeleção de GenesMasculinoCamundongosCamundongos Endogâmicos BALB CPeptidil Dipeptidase A/metabolismoReceptores da Bradicinina/genéticaReceptores da Bradicinina/metabolismoFatores de Tempoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8398/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSvensjo, Erik- Interplay between parasite........pdfSvensjo, Erik- Interplay between parasite........pdfapplication/pdf540364https://www.arca.fiocruz.br/bitstream/icict/8398/2/Svensjo%2c%20Erik-%20Interplay%20between%20parasite........pdfbe1082889aacb96e0eaada59cccfb538MD52TEXTSvensjo, Erik- Interplay between parasite........pdf.txtSvensjo, Erik- Interplay between parasite........pdf.txtExtracted texttext/plain80872https://www.arca.fiocruz.br/bitstream/icict/8398/3/Svensjo%2c%20Erik-%20Interplay%20between%20parasite........pdf.txt213c3199c6265be355b7fb754f6f12b9MD53icict/83982023-03-15 14:33:18.527oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
title	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
spellingShingle	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex. Svensjö, Nils Erik Leishmaniasis Innate immunity Inflammation Macrophages Endothelium Kinins Angiotensin-converting enzyme Cysteine proteases Permeabilidade Capilar/fisiologia Cisteína Endopeptidases/metabolismo Cininas/metabolismo Leishmania donovani/enzimologia Leishmania infantum/enzimologia Macrófagos/metabolismo Animais Cricetinae Deleção de Genes Masculino Camundongos Camundongos Endogâmicos BALB C Peptidil Dipeptidase A/metabolismo Receptores da Bradicinina/genética Receptores da Bradicinina/metabolismo Fatores de Tempo
title_short	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
title_full	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
title_fullStr	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
title_full_unstemmed	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
title_sort	Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.
author	Svensjö, Nils Erik
author_facet	Svensjö, Nils Erik Batista, Paulo Ricardo Brodskyn, Claudia Ida Silva, Robson Amaro Augusto da Lima, Ana Paula Cabral de Araujo Pereira, Verônica Schmitz Bou Habib, Elvira Maria Saraiva Chequer Pesquero, João Bosco Mori, Marcelo Alves da Silva Müller-Esterl, Werner Scharfstein, Julio
author_role	author
author2	Batista, Paulo Ricardo Brodskyn, Claudia Ida Silva, Robson Amaro Augusto da Lima, Ana Paula Cabral de Araujo Pereira, Verônica Schmitz Bou Habib, Elvira Maria Saraiva Chequer Pesquero, João Bosco Mori, Marcelo Alves da Silva Müller-Esterl, Werner Scharfstein, Julio
author2_role	author author author author author author author author author author
dc.contributor.author.fl_str_mv	Svensjö, Nils Erik Batista, Paulo Ricardo Brodskyn, Claudia Ida Silva, Robson Amaro Augusto da Lima, Ana Paula Cabral de Araujo Pereira, Verônica Schmitz Bou Habib, Elvira Maria Saraiva Chequer Pesquero, João Bosco Mori, Marcelo Alves da Silva Müller-Esterl, Werner Scharfstein, Julio
dc.subject.en.pt_BR.fl_str_mv	Leishmaniasis Innate immunity Inflammation Macrophages Endothelium Kinins Angiotensin-converting enzyme Cysteine proteases
topic	Leishmaniasis Innate immunity Inflammation Macrophages Endothelium Kinins Angiotensin-converting enzyme Cysteine proteases Permeabilidade Capilar/fisiologia Cisteína Endopeptidases/metabolismo Cininas/metabolismo Leishmania donovani/enzimologia Leishmania infantum/enzimologia Macrófagos/metabolismo Animais Cricetinae Deleção de Genes Masculino Camundongos Camundongos Endogâmicos BALB C Peptidil Dipeptidase A/metabolismo Receptores da Bradicinina/genética Receptores da Bradicinina/metabolismo Fatores de Tempo
dc.subject.decs.pt_BR.fl_str_mv	Permeabilidade Capilar/fisiologia Cisteína Endopeptidases/metabolismo Cininas/metabolismo Leishmania donovani/enzimologia Leishmania infantum/enzimologia Macrófagos/metabolismo Animais Cricetinae Deleção de Genes Masculino Camundongos Camundongos Endogâmicos BALB C Peptidil Dipeptidase A/metabolismo Receptores da Bradicinina/genética Receptores da Bradicinina/metabolismo Fatores de Tempo
description	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate	2006
dc.date.issued.fl_str_mv	2006
dc.date.accessioned.fl_str_mv	2014-09-18T16:18:30Z
dc.date.available.fl_str_mv	2014-09-18T16:18:30Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SVENSJO, E. et al. Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex. Microbes Infection, v.8, n. 1, p. 206-220, 2006.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/8398
dc.identifier.issn.none.fl_str_mv	1286-4579
dc.identifier.doi.none.fl_str_mv	10.1016/j.micinf.2005.06.016
identifier_str_mv	SVENSJO, E. et al. Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex. Microbes Infection, v.8, n. 1, p. 206-220, 2006. 1286-4579 10.1016/j.micinf.2005.06.016
url	https://www.arca.fiocruz.br/handle/icict/8398
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Elsevier SAS
publisher.none.fl_str_mv	Elsevier SAS
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
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institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Interplay between parasite cysteine proteases and the host kinin system modulates microvascular leakage and macrophage infection by promastigotes of the Leishmania donovani complex.

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