Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803

Detalhes bibliográficos
Autor(a) principal: Paulo AJ
Data de Publicação: 2021
Outros Autores: Wanderley MCA, de Oliveira RJV, Vieira WADS, Alves LC, Viana Marques DA, Converti A, Porto ALF.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58106
Resumo: Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Recife, PE, Brasil. Instituto Federal de Educação, Ciência e Tecnologia da Amazônia (IFAM), Tefé, Brasil. Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil. Universidade Federal de Pernambuco, Departamento de Micologia Prof. Chaves Batista, Recife, PE, Brasil. Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, PE, Brasil. Universidade de Pernambuco, Instituto de Ciências Biológicas, Laboratório de Biotecnologia Aplicada às Doenças Infecciosas e Parasitárias, Recife, PE, Brasil. Departamento de Engenharia Civil, Química e Ambiental, Polo de Engenharia Química, Gênova, Itália. Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, PE, Brasil
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spelling Paulo AJWanderley MCAde Oliveira RJVVieira WADSAlves LCViana Marques DAConverti APorto ALF.2023-05-02T15:06:51Z2023-05-02T15:06:51Z2021PAULO, Anderson José; WANDERLEY, Maria Carolina de Albuquerque; OLIVEIRA, Rafael José Vilela de; VIEIRA, Willie Anderson dos Santos; ALVES, Luiz Carlos; MARQUES, Daniela de Araújo Viana; CONVERTI, Attilio; PORTO, Ana Lúcia Figueiredo. Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803. Preparative Biochemistry & Biotechnology, [S.L.], v. 51, n. 3, p. 289-299, 9 set. 2020. Informa UK Limited. http://dx.doi.org/10.1080/10826068.2020.1815054.1532-2297https://www.arca.fiocruz.br/handle/icict/5810610.1080/10826068.2020.1815054porProduction and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz, Instituto Aggeu Magalhães, Recife, PE, Brasil. Instituto Federal de Educação, Ciência e Tecnologia da Amazônia (IFAM), Tefé, Brasil. Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil. Universidade Federal de Pernambuco, Departamento de Micologia Prof. Chaves Batista, Recife, PE, Brasil. Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, PE, Brasil. Universidade de Pernambuco, Instituto de Ciências Biológicas, Laboratório de Biotecnologia Aplicada às Doenças Infecciosas e Parasitárias, Recife, PE, Brasil. Departamento de Engenharia Civil, Química e Ambiental, Polo de Engenharia Química, Gênova, Itália. Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, PE, Brasilβ-Galactosidase production, partial purification and characterization by a new fungal were investigated. Partial purification was performed by aqueous two-phase system (ATPS) using polyethylene glycol (PEG) molar mass, PEG concentration, citrate concentration and pH as the independent variables. Purification factor (PF), partition coefficient (K) and yield (Y) were the responses. After identification by rDNA sequencing and classification as Cladosporium tenuissimum URM 7803, this isolate achieved a maximum cell concentration and β-galactosidase activity of 0.48 g/L and 462.1 U/mL, respectively. β-Galactosidase partitioned preferentially for bottom salt-rich phase likely due to hydrophobicity and volume exclusion effect caused in the top phase by the high PEG concentration and molar mass. The highest value of PF (12.94) was obtained using 24% (w/w) PEG 8000 g/mol and 15% (w/w) citrate, while that of Y (79.76%) using 20% (w/w) PEG 400 g/mol and 25% (w/w) citrate, both at pH 6. The enzyme exhibited optimum temperature in crude and ATPS extracts in the ranges 35–50 °C and 40–55 °C, respectively, and optimum pH in the range 3.0–4.5, with a fall of enzyme activity under alkaline conditions. Some metal ions and detergents inhibited, while others stimulated enzyme activity. Finally, C. tenuissimum URM 7803 β-galactosidase showed a profile suitable for prebiotics production.β-Galactosidasefungiphylogenesisproductionaqueous two-phase systeminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALProduction and partial purification by PEG citrate ATPS of a galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803.pdfProduction and partial purification by PEG citrate ATPS of a galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803.pdfapplication/pdf2099489https://www.arca.fiocruz.br/bitstream/icict/58106/2/Production%20and%20partial%20purification%20by%20PEG%20citrate%20ATPS%20of%20a%20galactosidase%20from%20the%20new%20promising%20isolate%20Cladosporium%20tenuissimum%20URM%207803.pdfcb69a67a4bbaf92ad08098915a2b4c87MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58106/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/581062023-05-30 12:27:54.563oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-30T15:27:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
title Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
spellingShingle Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
Paulo AJ
β-Galactosidase
fungi
phylogenesis
production
aqueous two-phase system
title_short Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
title_full Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
title_fullStr Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
title_full_unstemmed Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
title_sort Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803
author Paulo AJ
author_facet Paulo AJ
Wanderley MCA
de Oliveira RJV
Vieira WADS
Alves LC
Viana Marques DA
Converti A
Porto ALF.
author_role author
author2 Wanderley MCA
de Oliveira RJV
Vieira WADS
Alves LC
Viana Marques DA
Converti A
Porto ALF.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paulo AJ
Wanderley MCA
de Oliveira RJV
Vieira WADS
Alves LC
Viana Marques DA
Converti A
Porto ALF.
dc.subject.en.en_US.fl_str_mv β-Galactosidase
fungi
phylogenesis
production
aqueous two-phase system
topic β-Galactosidase
fungi
phylogenesis
production
aqueous two-phase system
description Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Recife, PE, Brasil. Instituto Federal de Educação, Ciência e Tecnologia da Amazônia (IFAM), Tefé, Brasil. Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil. Universidade Federal de Pernambuco, Departamento de Micologia Prof. Chaves Batista, Recife, PE, Brasil. Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, PE, Brasil. Universidade de Pernambuco, Instituto de Ciências Biológicas, Laboratório de Biotecnologia Aplicada às Doenças Infecciosas e Parasitárias, Recife, PE, Brasil. Departamento de Engenharia Civil, Química e Ambiental, Polo de Engenharia Química, Gênova, Itália. Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, PE, Brasil
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2023-05-02T15:06:51Z
dc.date.available.fl_str_mv 2023-05-02T15:06:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PAULO, Anderson José; WANDERLEY, Maria Carolina de Albuquerque; OLIVEIRA, Rafael José Vilela de; VIEIRA, Willie Anderson dos Santos; ALVES, Luiz Carlos; MARQUES, Daniela de Araújo Viana; CONVERTI, Attilio; PORTO, Ana Lúcia Figueiredo. Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803. Preparative Biochemistry & Biotechnology, [S.L.], v. 51, n. 3, p. 289-299, 9 set. 2020. Informa UK Limited. http://dx.doi.org/10.1080/10826068.2020.1815054.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58106
dc.identifier.issn.en_US.fl_str_mv 1532-2297
dc.identifier.doi.none.fl_str_mv 10.1080/10826068.2020.1815054
identifier_str_mv PAULO, Anderson José; WANDERLEY, Maria Carolina de Albuquerque; OLIVEIRA, Rafael José Vilela de; VIEIRA, Willie Anderson dos Santos; ALVES, Luiz Carlos; MARQUES, Daniela de Araújo Viana; CONVERTI, Attilio; PORTO, Ana Lúcia Figueiredo. Production and partial purification by PEG/citrate ATPS of a β-galactosidase from the new promising isolate Cladosporium tenuissimum URM 7803. Preparative Biochemistry & Biotechnology, [S.L.], v. 51, n. 3, p. 289-299, 9 set. 2020. Informa UK Limited. http://dx.doi.org/10.1080/10826068.2020.1815054.
1532-2297
10.1080/10826068.2020.1815054
url https://www.arca.fiocruz.br/handle/icict/58106
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58106/2/Production%20and%20partial%20purification%20by%20PEG%20citrate%20ATPS%20of%20a%20galactosidase%20from%20the%20new%20promising%20isolate%20Cladosporium%20tenuissimum%20URM%207803.pdf
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