Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans

Detalhes bibliográficos
Autor(a) principal: Fleith, Renata
Data de Publicação: 2016
Outros Autores: Lobo, Francisco, Santos, Paula Fernandes dos, Rocha, Mariana, Bordignon, Juliano, Strottmann, Daisy Maria, Patricio, Daniel, Pavanelli, Wander Rogério, Sarzi, Maria Lo, Santos, Claudia Nunes Duarte dos, Ferguson, Brian, Mansur, Daniel Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20442
Resumo: Laboratory of Immunobiology. Department of Microbiology. Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.
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spelling Fleith, RenataLobo, FranciscoSantos, Paula Fernandes dosRocha, MarianaBordignon, JulianoStrottmann, Daisy MariaPatricio, DanielPavanelli, Wander RogérioSarzi, Maria LoSantos, Claudia Nunes Duarte dosFerguson, BrianMansur, Daniel Santos2017-07-28T14:00:29Z2017-07-28T14:00:29Z2016FLEITH, Renata C. et al. Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans. Sci. Rep., v. 6, n. 36339, p. 1-8, 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/2044210.1038/ srep36339engNature Publishing GroupVírus da DengueDengue VirusAmino Acid SequenceBase SequenceAntigen-Antibody ReactionsVirus del DengueSecuencia de AminoácidosSecuencia de BasesReacciones Antígeno-AnticuerpoSequência de AminoácidosSequência de BasesReações Antígeno-AnticorpoGenome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratory of Immunobiology. Department of Microbiology. Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.Department of General Biology. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil.Laboratory of Immunobiology. Department of Microbiology, Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.Laboratory of Immunobiology. Department of Microbiology, Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Laboratory of Immunobiology. Department of Microbiology. Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.Department of Pathology. Universidade Estadual de Londrina. Londrina, PR, Brazil.Secretary for Health. Cambé, PR, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Department of Pathology. University of Cambridge. Cambridge, UK.Laboratory of Immunobiology. Department of Microbiology. Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.Targeting regions of proteins that show a high degree of structural conservation has been proposed as a method of developing immunotherapies and vaccines that may bypass the wide genetic variability of RNA viruses. Despite several attempts, a vaccine that protects evenly against the four circulating Dengue virus (DV) serotypes remains elusive. To find critical conserved amino acids in dengue viruses, 120 complete genomes of each serotype were selected at random and used to calculate conservation scores for nucleotide and amino acid sequences. The identified peptide sequences were analysed for their structural conservation and localisation using crystallographic data. The longest, surface exposed, highly conserved peptide of Envelope protein was found to correspond to amino acid residues 250 to 270. Mutation of this peptide in DV1 was lethal, since no replication of the mutant virus was detected in human cells. Antibodies against this peptide were detected in DV naturally infected patients indicating its potential antigenicity. Hence, this study has identified a highly conserved, critical peptide in DV that is a target of antibodies in infected humans.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20442/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGenome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans.pdfGenome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans.pdfapplication/pdf1326844https://www.arca.fiocruz.br/bitstream/icict/20442/2/Genome-wide%20analyses%20reveal%20a%20highly%20conserved%20Dengue%20virus%20envelope%20peptide%20which%20is%20critical%20for%20virus%20viability%20and%20antigenic%20in%20humans.pdfad4d1bcd614fc7bbcd39eb9cea4f54e0MD52TEXTGenome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans.pdf.txtGenome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans.pdf.txtExtracted texttext/plain31529https://www.arca.fiocruz.br/bitstream/icict/20442/3/Genome-wide%20analyses%20reveal%20a%20highly%20conserved%20Dengue%20virus%20envelope%20peptide%20which%20is%20critical%20for%20virus%20viability%20and%20antigenic%20in%20humans.pdf.txtb85fdf53a1d48a1acac7f5e9155d2302MD53icict/204422018-08-15 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dc.title.pt_BR.fl_str_mv Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
title Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
spellingShingle Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
Fleith, Renata
Vírus da Dengue
Dengue Virus
Amino Acid Sequence
Base Sequence
Antigen-Antibody Reactions
Virus del Dengue
Secuencia de Aminoácidos
Secuencia de Bases
Reacciones Antígeno-Anticuerpo
Sequência de Aminoácidos
Sequência de Bases
Reações Antígeno-Anticorpo
title_short Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
title_full Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
title_fullStr Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
title_full_unstemmed Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
title_sort Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans
author Fleith, Renata
author_facet Fleith, Renata
Lobo, Francisco
Santos, Paula Fernandes dos
Rocha, Mariana
Bordignon, Juliano
Strottmann, Daisy Maria
Patricio, Daniel
Pavanelli, Wander Rogério
Sarzi, Maria Lo
Santos, Claudia Nunes Duarte dos
Ferguson, Brian
Mansur, Daniel Santos
author_role author
author2 Lobo, Francisco
Santos, Paula Fernandes dos
Rocha, Mariana
Bordignon, Juliano
Strottmann, Daisy Maria
Patricio, Daniel
Pavanelli, Wander Rogério
Sarzi, Maria Lo
Santos, Claudia Nunes Duarte dos
Ferguson, Brian
Mansur, Daniel Santos
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fleith, Renata
Lobo, Francisco
Santos, Paula Fernandes dos
Rocha, Mariana
Bordignon, Juliano
Strottmann, Daisy Maria
Patricio, Daniel
Pavanelli, Wander Rogério
Sarzi, Maria Lo
Santos, Claudia Nunes Duarte dos
Ferguson, Brian
Mansur, Daniel Santos
dc.subject.other.pt_BR.fl_str_mv Vírus da Dengue
topic Vírus da Dengue
Dengue Virus
Amino Acid Sequence
Base Sequence
Antigen-Antibody Reactions
Virus del Dengue
Secuencia de Aminoácidos
Secuencia de Bases
Reacciones Antígeno-Anticuerpo
Sequência de Aminoácidos
Sequência de Bases
Reações Antígeno-Anticorpo
dc.subject.en.pt_BR.fl_str_mv Dengue Virus
Amino Acid Sequence
Base Sequence
Antigen-Antibody Reactions
dc.subject.es.pt_BR.fl_str_mv Virus del Dengue
Secuencia de Aminoácidos
Secuencia de Bases
Reacciones Antígeno-Anticuerpo
dc.subject.decs.pt_BR.fl_str_mv Sequência de Aminoácidos
Sequência de Bases
Reações Antígeno-Anticorpo
description Laboratory of Immunobiology. Department of Microbiology. Immunology and Parasitology. Universidade Federal de Santa Catarina. Florianópolis, SC, Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-07-28T14:00:29Z
dc.date.available.fl_str_mv 2017-07-28T14:00:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FLEITH, Renata C. et al. Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans. Sci. Rep., v. 6, n. 36339, p. 1-8, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20442
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.pt_BR.fl_str_mv 10.1038/ srep36339
identifier_str_mv FLEITH, Renata C. et al. Genome-wide analyses reveal a highly conserved Dengue virus envelope peptide which is critical for virus viability and antigenic in humans. Sci. Rep., v. 6, n. 36339, p. 1-8, 2016.
2045-2322
10.1038/ srep36339
url https://www.arca.fiocruz.br/handle/icict/20442
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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