Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.

Detalhes bibliográficos
Autor(a) principal: Wanderley, João Luiz Mendes
Data de Publicação: 2009
Outros Autores: Silva, Lucia Helena Pinto da, Deolindo, Poliana, Soong, Lynn, Borges, Valeria de Matos, Prates, Deboraci Brito, Souza, Ana Paula Almeida de, Barral, Aldina Maria Prado, Balanco, José Mario de Freitas, Nascimento, Michelle Tanny Cunha do, Saraiva, Elvira Maria B, Barcinski, Marcello André
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9467
Resumo: National Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / Federal University of Rio de Janeiro. Biomedical Sciences Institute. Morphological Sciences Program. Rio de Janeiro, RJ, Brasil
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spelling Wanderley, João Luiz MendesSilva, Lucia Helena Pinto daDeolindo, PolianaSoong, LynnBorges, Valeria de MatosPrates, Deboraci BritoSouza, Ana Paula Almeida deBarral, Aldina Maria PradoBalanco, José Mario de FreitasNascimento, Michelle Tanny Cunha doSaraiva, Elvira Maria BBarcinski, Marcello André2015-02-09T18:43:44Z2015-02-09T18:43:44Z2009WANDERLEY, J. L. M. et al. Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis. PLoS One, v. 4, n. 5, p. e5733, 2009.1932-6203https://www.arca.fiocruz.br/handle/icict/946710.1371/journal.pone.0005733engPublic Library of ScienceCooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / Federal University of Rio de Janeiro. Biomedical Sciences Institute. Morphological Sciences Program. Rio de Janeiro, RJ, BrasilNational Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Microbiology and Veterinary Immunology. Rio de Janeiro, RJ, BrasilNational Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Molecular and Cellular Biology Program. Rio de Janeiro, RJ, BrasilUniversity of Texas Medical Branch. Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development. Departments of Microbiology and Immunology and of Pathology. Galveston, Texas, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Sao Paulo. Parasitology Department. Biomedical Sciences Institute. Sao Paulo, SP, BrasilFederal University of Rio de Janeiro. Microbiology Institute. Immunology Department. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. Microbiology Institute. Immunology Department. Rio de Janeiro, RJ, BrasilNational Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / University of Sao Paulo. Parasitology Department. Biomedical Sciences Institute. Sao Paulo, SP, BrasilMimicking mammalian apoptotic cells by exposing phosphatidylserine (PS) is a strategy used by virus and parasitic protozoa to escape host protective inflammatory responses. With Leishmania amazonensis (La), apoptotic mimicry is a prerogative of the intramacrophagic amastigote form of the parasite and is modulated by the host. Now we show that differently from what happens with amastigotes, promastigotes exposing PS are non-viable, non-infective cells, undergoing apoptotic death. As part of the normal metacyclogenic process occurring in axenic cultures and in the gut of sand fly vectors, a subpopulation of metacyclic promastigotes exposes PS. Apoptotic death of the purified PS-positive (PSPOS) sub-population was confirmed by TUNEL staining and DNA laddering. Transmission electron microscopy revealed morphological alterations in PSPOS metacyclics such as DNA condensation, cytoplasm degradation and mitochondrion and kinetoplast destruction, both in in vitro cultures and in sand fly guts. TUNELPOS promastigotes were detected only in the anterior midgut to foregut boundary of infected sand flies. Interestingly, caspase inhibitors modulated parasite death and PS exposure, when added to parasite cultures in a specific time window. Efficient in vitro macrophage infections and in vivo lesions only occur when PSPOS and PS-negative (PSNEG) parasites were simultaneously added to the cell culture or inoculated in the mammalian host. The viable PSNEG promastigote was the infective form, as shown by following the fate of fluorescently labeled parasites, while the PSPOS apoptotic sub-population inhibited host macrophage inflammatory response. PS exposure and macrophage inhibition by a subpopulation of promastigotes is a different mechanism than the one previously described with amastigotes, where the entire population exposes PS. Both mechanisms co-exist and play a role in the transmission and development of the disease in case of infection by La. Since both processes confer selective advantages to the infective microorganism they justify the occurrence of apoptotic features in a unicellular pathogen.ApoptoseLeishmania mexicana/citologiaLeishmania mexicana/crescimento & desenvolvimentoLeishmaniose/patologiaLeishmaniose/parasitologiaEstágios do Ciclo de VidaAnimaisSistema Digestório/citologiaMarcação In Situ das Extremidades CortadasLeishmania mexicana/patogenicidadeCamundongosFosfatidilserinas/metabolismoPsychodidae/citologiaPsychodidae/parasitologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9467/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALWanderley L M J Cooperation between....pdfWanderley L M J Cooperation between....pdfapplication/pdf1753136https://www.arca.fiocruz.br/bitstream/icict/9467/2/Wanderley%20L%20M%20J%20Cooperation%20between....pdf793d9e5abf0456451ccffd30dd01d9ffMD52TEXTWanderley L M J Cooperation between....pdf.txtWanderley L M J Cooperation between....pdf.txtExtracted texttext/plain55257https://www.arca.fiocruz.br/bitstream/icict/9467/3/Wanderley%20L%20M%20J%20Cooperation%20between....pdf.txt89b5f3a73152f48c9e24da7495b6ce97MD53icict/94672023-03-15 14:33:04.082oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
title Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
spellingShingle Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
Wanderley, João Luiz Mendes
Apoptose
Leishmania mexicana/citologia
Leishmania mexicana/crescimento & desenvolvimento
Leishmaniose/patologia
Leishmaniose/parasitologia
Estágios do Ciclo de Vida
Animais
Sistema Digestório/citologia
Marcação In Situ das Extremidades Cortadas
Leishmania mexicana/patogenicidade
Camundongos
Fosfatidilserinas/metabolismo
Psychodidae/citologia
Psychodidae/parasitologia
title_short Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
title_full Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
title_fullStr Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
title_full_unstemmed Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
title_sort Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis.
author Wanderley, João Luiz Mendes
author_facet Wanderley, João Luiz Mendes
Silva, Lucia Helena Pinto da
Deolindo, Poliana
Soong, Lynn
Borges, Valeria de Matos
Prates, Deboraci Brito
Souza, Ana Paula Almeida de
Barral, Aldina Maria Prado
Balanco, José Mario de Freitas
Nascimento, Michelle Tanny Cunha do
Saraiva, Elvira Maria B
Barcinski, Marcello André
author_role author
author2 Silva, Lucia Helena Pinto da
Deolindo, Poliana
Soong, Lynn
Borges, Valeria de Matos
Prates, Deboraci Brito
Souza, Ana Paula Almeida de
Barral, Aldina Maria Prado
Balanco, José Mario de Freitas
Nascimento, Michelle Tanny Cunha do
Saraiva, Elvira Maria B
Barcinski, Marcello André
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wanderley, João Luiz Mendes
Silva, Lucia Helena Pinto da
Deolindo, Poliana
Soong, Lynn
Borges, Valeria de Matos
Prates, Deboraci Brito
Souza, Ana Paula Almeida de
Barral, Aldina Maria Prado
Balanco, José Mario de Freitas
Nascimento, Michelle Tanny Cunha do
Saraiva, Elvira Maria B
Barcinski, Marcello André
dc.subject.decs.pt_BR.fl_str_mv Apoptose
Leishmania mexicana/citologia
Leishmania mexicana/crescimento & desenvolvimento
Leishmaniose/patologia
Leishmaniose/parasitologia
Estágios do Ciclo de Vida
Animais
Sistema Digestório/citologia
Marcação In Situ das Extremidades Cortadas
Leishmania mexicana/patogenicidade
Camundongos
Fosfatidilserinas/metabolismo
Psychodidae/citologia
Psychodidae/parasitologia
topic Apoptose
Leishmania mexicana/citologia
Leishmania mexicana/crescimento & desenvolvimento
Leishmaniose/patologia
Leishmaniose/parasitologia
Estágios do Ciclo de Vida
Animais
Sistema Digestório/citologia
Marcação In Situ das Extremidades Cortadas
Leishmania mexicana/patogenicidade
Camundongos
Fosfatidilserinas/metabolismo
Psychodidae/citologia
Psychodidae/parasitologia
description National Cancer Institute. Experimental Medicine Division. Rio de Janeiro, RJ, Brasil / Federal University of Rio de Janeiro. Biomedical Sciences Institute. Morphological Sciences Program. Rio de Janeiro, RJ, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2015-02-09T18:43:44Z
dc.date.available.fl_str_mv 2015-02-09T18:43:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WANDERLEY, J. L. M. et al. Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis. PLoS One, v. 4, n. 5, p. e5733, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9467
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0005733
identifier_str_mv WANDERLEY, J. L. M. et al. Cooperation between apoptotic and viable metacyclics enhances the pathogenesis of Leishmaniasis. PLoS One, v. 4, n. 5, p. e5733, 2009.
1932-6203
10.1371/journal.pone.0005733
url https://www.arca.fiocruz.br/handle/icict/9467
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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