Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33980 |
Resumo: | Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil. |
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Vidal, José E.Toniolo, CarolinaPaulino, AdrianaColombo, Arnaldo L.Martins, Marilena dos AnjosMeira, Cristina da SilvaAzevedo, Renata Guise SoaresPereira-Chioccola, Vera LuciaGomes, Hélio RodriguesLazera, Marcia dos SantosOliveira, Augusto C. Penalva deBoulware, David R.2019-07-11T13:40:39Z2019-07-11T13:40:39Z2018VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018.0036-4665https://www.arca.fiocruz.br/handle/icict/3398010.1590/s1678-99462018600011678-9946engUniversidade de São PauloPerformance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral centerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Universidade Federal de São Paulo. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.University of Minnesota. Minneapolis, MN, USA.Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum. Twenty patients with cryptococcal meningitis were included and in 19 of them, CrAg LFA in CSF, serum, and whole blood were positive (95% sensitivity). In 18 patients, India ink test was positive in CSF (90% sensitivity), and in 16 cases, CrAg LFA was positive in urine (80% sensitivity). Thirty-six HIV-infected patients with other neurological diseases had negative results of CrAg LFA in serum (100% specificity). In conclusion, CrAg LFA in serum, CSF, and whole blood showed high sensitivity and specificity. Whole blood CrAg LFA seems to be a good and reliable strategy to improve AIDS-related cryptococcal meningitis diagnosis in Brazil.Cryptococcal meningitisCryptococcosisDiagnosisHuman immunodeficiency virusAcquired immunodeficiency syndromeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/33980/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Vidal_José_etal_INI_2018.pdfve_Vidal_José_etal_INI_2018.pdfapplication/pdf107646https://www.arca.fiocruz.br/bitstream/icict/33980/2/ve_Vidal_Jos%c3%a9_etal_INI_2018.pdf9b6d643f487a693844d2a70c581d3674MD52TEXTve_Vidal_José_etal_INI_2018.pdf.txtve_Vidal_José_etal_INI_2018.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| title |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| spellingShingle |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center Vidal, José E. Cryptococcal meningitis Cryptococcosis Diagnosis Human immunodeficiency virus Acquired immunodeficiency syndrome |
| title_short |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| title_full |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| title_fullStr |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| title_full_unstemmed |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| title_sort |
Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center |
| author |
Vidal, José E. |
| author_facet |
Vidal, José E. Toniolo, Carolina Paulino, Adriana Colombo, Arnaldo L. Martins, Marilena dos Anjos Meira, Cristina da Silva Azevedo, Renata Guise Soares Pereira-Chioccola, Vera Lucia Gomes, Hélio Rodrigues Lazera, Marcia dos Santos Oliveira, Augusto C. Penalva de Boulware, David R. |
| author_role |
author |
| author2 |
Toniolo, Carolina Paulino, Adriana Colombo, Arnaldo L. Martins, Marilena dos Anjos Meira, Cristina da Silva Azevedo, Renata Guise Soares Pereira-Chioccola, Vera Lucia Gomes, Hélio Rodrigues Lazera, Marcia dos Santos Oliveira, Augusto C. Penalva de Boulware, David R. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vidal, José E. Toniolo, Carolina Paulino, Adriana Colombo, Arnaldo L. Martins, Marilena dos Anjos Meira, Cristina da Silva Azevedo, Renata Guise Soares Pereira-Chioccola, Vera Lucia Gomes, Hélio Rodrigues Lazera, Marcia dos Santos Oliveira, Augusto C. Penalva de Boulware, David R. |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptococcal meningitis Cryptococcosis Diagnosis Human immunodeficiency virus Acquired immunodeficiency syndrome |
| topic |
Cryptococcal meningitis Cryptococcosis Diagnosis Human immunodeficiency virus Acquired immunodeficiency syndrome |
| description |
Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil. |
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2018 |
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2018 |
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2019-07-11T13:40:39Z |
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2019-07-11T13:40:39Z |
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info:eu-repo/semantics/publishedVersion |
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VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018. |
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https://www.arca.fiocruz.br/handle/icict/33980 |
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0036-4665 |
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10.1590/s1678-9946201860001 |
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1678-9946 |
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VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018. 0036-4665 10.1590/s1678-9946201860001 1678-9946 |
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Universidade de São Paulo |
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Universidade de São Paulo |
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