Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center

Detalhes bibliográficos
Autor(a) principal: Vidal, José E.
Data de Publicação: 2018
Outros Autores: Toniolo, Carolina, Paulino, Adriana, Colombo, Arnaldo L., Martins, Marilena dos Anjos, Meira, Cristina da Silva, Azevedo, Renata Guise Soares, Pereira-Chioccola, Vera Lucia, Gomes, Hélio Rodrigues, Lazera, Marcia dos Santos, Oliveira, Augusto C. Penalva de, Boulware, David R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33980
Resumo: Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
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spelling Vidal, José E.Toniolo, CarolinaPaulino, AdrianaColombo, Arnaldo L.Martins, Marilena dos AnjosMeira, Cristina da SilvaAzevedo, Renata Guise SoaresPereira-Chioccola, Vera LuciaGomes, Hélio RodriguesLazera, Marcia dos SantosOliveira, Augusto C. Penalva deBoulware, David R.2019-07-11T13:40:39Z2019-07-11T13:40:39Z2018VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018.0036-4665https://www.arca.fiocruz.br/handle/icict/3398010.1590/s1678-99462018600011678-9946engUniversidade de São PauloPerformance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral centerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Universidade Federal de São Paulo. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil.University of Minnesota. Minneapolis, MN, USA.Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum. Twenty patients with cryptococcal meningitis were included and in 19 of them, CrAg LFA in CSF, serum, and whole blood were positive (95% sensitivity). In 18 patients, India ink test was positive in CSF (90% sensitivity), and in 16 cases, CrAg LFA was positive in urine (80% sensitivity). Thirty-six HIV-infected patients with other neurological diseases had negative results of CrAg LFA in serum (100% specificity). In conclusion, CrAg LFA in serum, CSF, and whole blood showed high sensitivity and specificity. Whole blood CrAg LFA seems to be a good and reliable strategy to improve AIDS-related cryptococcal meningitis diagnosis in Brazil.Cryptococcal meningitisCryptococcosisDiagnosisHuman immunodeficiency virusAcquired immunodeficiency syndromeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/33980/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Vidal_José_etal_INI_2018.pdfve_Vidal_José_etal_INI_2018.pdfapplication/pdf107646https://www.arca.fiocruz.br/bitstream/icict/33980/2/ve_Vidal_Jos%c3%a9_etal_INI_2018.pdf9b6d643f487a693844d2a70c581d3674MD52TEXTve_Vidal_José_etal_INI_2018.pdf.txtve_Vidal_José_etal_INI_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
title Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
spellingShingle Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
Vidal, José E.
Cryptococcal meningitis
Cryptococcosis
Diagnosis
Human immunodeficiency virus
Acquired immunodeficiency syndrome
title_short Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
title_full Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
title_fullStr Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
title_full_unstemmed Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
title_sort Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center
author Vidal, José E.
author_facet Vidal, José E.
Toniolo, Carolina
Paulino, Adriana
Colombo, Arnaldo L.
Martins, Marilena dos Anjos
Meira, Cristina da Silva
Azevedo, Renata Guise Soares
Pereira-Chioccola, Vera Lucia
Gomes, Hélio Rodrigues
Lazera, Marcia dos Santos
Oliveira, Augusto C. Penalva de
Boulware, David R.
author_role author
author2 Toniolo, Carolina
Paulino, Adriana
Colombo, Arnaldo L.
Martins, Marilena dos Anjos
Meira, Cristina da Silva
Azevedo, Renata Guise Soares
Pereira-Chioccola, Vera Lucia
Gomes, Hélio Rodrigues
Lazera, Marcia dos Santos
Oliveira, Augusto C. Penalva de
Boulware, David R.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vidal, José E.
Toniolo, Carolina
Paulino, Adriana
Colombo, Arnaldo L.
Martins, Marilena dos Anjos
Meira, Cristina da Silva
Azevedo, Renata Guise Soares
Pereira-Chioccola, Vera Lucia
Gomes, Hélio Rodrigues
Lazera, Marcia dos Santos
Oliveira, Augusto C. Penalva de
Boulware, David R.
dc.subject.en.pt_BR.fl_str_mv Cryptococcal meningitis
Cryptococcosis
Diagnosis
Human immunodeficiency virus
Acquired immunodeficiency syndrome
topic Cryptococcal meningitis
Cryptococcosis
Diagnosis
Human immunodeficiency virus
Acquired immunodeficiency syndrome
description Instituto de Infectologia Emilio Ribas. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-11T13:40:39Z
dc.date.available.fl_str_mv 2019-07-11T13:40:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33980
dc.identifier.issn.pt_BR.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv 10.1590/s1678-9946201860001
dc.identifier.eissn.none.fl_str_mv 1678-9946
identifier_str_mv VIDAL, José E. et al. Performance of cryptococcal antigen lateral flow assay in serum, cerebrospinal fluid, whole blood, and urine in HIV-infected patients with culture-proven cryptococcal meningitis admitted at a Brazilian referral center. Revista do Instituto de Medicina Tropical de São Paulo, v. 60, p. 1-3, 2018.
0036-4665
10.1590/s1678-9946201860001
1678-9946
url https://www.arca.fiocruz.br/handle/icict/33980
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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