Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37881 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil) within the call “CNPq/MS/SCTIE/DECIT N° 32/2014 - Pesquisas sobre Leishmanioses” grant number reference 467389/2014-4. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. TC received scholarship from Fundação de Amparo a Pesquisa do Estado de Santa Catarina – FAPESC. |
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Ramírez, LauraMoura, Luana Dias deMateus, Natalia Lopes FontouraMoraes, Milene Hoehr deNascimento, Leopoldo Fabrício Marçal doMelo, Nailson de JesusTaketa, Lucas BezerraCatecati, TatianaHuete, Samuel G.Penichet, KarlaPiranda, Eliane MattosOliveira, Alessandra Gutierrez deSteindel, MarioBarral Netto, ManoelCruz, Maria do Socorro Pires eBarral, Aldina Maria PradoSoto, Manuel2019-12-11T18:17:03Z2019-12-11T18:17:03Z2020RAMIREZ, Laura et al. Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence. Parasite Epidemiology and Control, v. 8, p. 1-12, Feb. 2020.2405-6731https://www.arca.fiocruz.br/handle/icict/3788110.1016/j.parepi.2019.e00126Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil) within the call “CNPq/MS/SCTIE/DECIT N° 32/2014 - Pesquisas sobre Leishmanioses” grant number reference 467389/2014-4. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. TC received scholarship from Fundação de Amparo a Pesquisa do Estado de Santa Catarina – FAPESC.Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Facultad de Ciencias. Departamento de Biología Molecular. Madrid, Spain.Universidade Federal do Piauí. Centro de Ciências Agrárias. Teresina, PI, Brasil.Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.Universidade Federal do Piauí. Centro de Ciências Agrárias. Teresina, PI, Brasil.Universidade Federal do Piauí. Centro de Ciências Agrárias. Teresina, PI, Brasil.Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Facultad de Ciencias. Departamento de Biología Molecular. Madrid, Spain.Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Facultad de Ciencias. Departamento de Biología Molecular. Madrid, Spain.Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Piauí. Centro de Ciências Agrárias. Teresina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa. Facultad de Ciencias. Departamento de Biología Molecular. Madrid, Spain.Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with sub-clinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extracts.engElsevierLeishmaniaLeishmaniose caninaSorodiagnósticoAnticorposProteínas recombinantesLeishmaniaCanine leishmaniasisSerodiagnosisAntibodiesRecombinant proteinsImproving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37881/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRamirez, L. Improving....pdfRamirez, L. Improving....pdfapplication/pdf977613https://www.arca.fiocruz.br/bitstream/icict/37881/2/Ramirez%2c%20L.%20Improving....pdf5456a28022765a30141dba514f89c95fMD52TEXTRamirez, L. Improving....pdf.txtRamirez, L. Improving....pdf.txtExtracted texttext/plain65823https://www.arca.fiocruz.br/bitstream/icict/37881/3/Ramirez%2c%20L.%20Improving....pdf.txt9a31da4b2305fda4e1f17121f36e24adMD53icict/378812023-03-15 14:34:19.277oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| title |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| spellingShingle |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence Ramírez, Laura Leishmania Leishmaniose canina Sorodiagnóstico Anticorpos Proteínas recombinantes Leishmania Canine leishmaniasis Serodiagnosis Antibodies Recombinant proteins |
| title_short |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| title_full |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| title_fullStr |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| title_full_unstemmed |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| title_sort |
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence |
| author |
Ramírez, Laura |
| author_facet |
Ramírez, Laura Moura, Luana Dias de Mateus, Natalia Lopes Fontoura Moraes, Milene Hoehr de Nascimento, Leopoldo Fabrício Marçal do Melo, Nailson de Jesus Taketa, Lucas Bezerra Catecati, Tatiana Huete, Samuel G. Penichet, Karla Piranda, Eliane Mattos Oliveira, Alessandra Gutierrez de Steindel, Mario Barral Netto, Manoel Cruz, Maria do Socorro Pires e Barral, Aldina Maria Prado Soto, Manuel |
| author_role |
author |
| author2 |
Moura, Luana Dias de Mateus, Natalia Lopes Fontoura Moraes, Milene Hoehr de Nascimento, Leopoldo Fabrício Marçal do Melo, Nailson de Jesus Taketa, Lucas Bezerra Catecati, Tatiana Huete, Samuel G. Penichet, Karla Piranda, Eliane Mattos Oliveira, Alessandra Gutierrez de Steindel, Mario Barral Netto, Manoel Cruz, Maria do Socorro Pires e Barral, Aldina Maria Prado Soto, Manuel |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ramírez, Laura Moura, Luana Dias de Mateus, Natalia Lopes Fontoura Moraes, Milene Hoehr de Nascimento, Leopoldo Fabrício Marçal do Melo, Nailson de Jesus Taketa, Lucas Bezerra Catecati, Tatiana Huete, Samuel G. Penichet, Karla Piranda, Eliane Mattos Oliveira, Alessandra Gutierrez de Steindel, Mario Barral Netto, Manoel Cruz, Maria do Socorro Pires e Barral, Aldina Maria Prado Soto, Manuel |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Leishmaniose canina Sorodiagnóstico Anticorpos Proteínas recombinantes |
| topic |
Leishmania Leishmaniose canina Sorodiagnóstico Anticorpos Proteínas recombinantes Leishmania Canine leishmaniasis Serodiagnosis Antibodies Recombinant proteins |
| dc.subject.en.en.fl_str_mv |
Leishmania Canine leishmaniasis Serodiagnosis Antibodies Recombinant proteins |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil) within the call “CNPq/MS/SCTIE/DECIT N° 32/2014 - Pesquisas sobre Leishmanioses” grant number reference 467389/2014-4. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. TC received scholarship from Fundação de Amparo a Pesquisa do Estado de Santa Catarina – FAPESC. |
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2019 |
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2019-12-11T18:17:03Z |
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2019-12-11T18:17:03Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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RAMIREZ, Laura et al. Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence. Parasite Epidemiology and Control, v. 8, p. 1-12, Feb. 2020. |
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https://www.arca.fiocruz.br/handle/icict/37881 |
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2405-6731 |
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10.1016/j.parepi.2019.e00126 |
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RAMIREZ, Laura et al. Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence. Parasite Epidemiology and Control, v. 8, p. 1-12, Feb. 2020. 2405-6731 10.1016/j.parepi.2019.e00126 |
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Elsevier |
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Elsevier |
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