Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19653 |
Resumo: | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. |
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Ferraz-Carvalho, Rafaela S.Pereira, Marcela A.Linhares, Leonardo A.Lira-Nogueira, Mariane C.Cavalcanti, Isabella MfSantos-Magalhães, Nereide S.Montenegro, Lílian M.2017-06-28T19:08:16Z2017-06-28T19:08:16Z2016FERRAZ-CARVALHO, R. S. et al. Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 5, p. 330–334, May 2016.1678-8060https://www.arca.fiocruz.br/handle/icict/1965310.1590/0074-02760150454engMDR-TBAtividade antimicobacterianaSinergismoÁcido usnicLipossomasMDR-TBAntimycobacterial activitySynergismUsnic acidLiposomesMycobacterium tuberculosis / Efeitos de drogasRifampicina / FarmacologiaAntibióticos Antituberculosos / FarmacologiaBenzofuranos / FarmacologiaIsoniazida / FarmacologiaLipossomasEffects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoepidemiologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico de Vitória. Laboratório de Microbiologia e Imunologia. Vitória de Santo Antão, PE, Brasil.Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunoepidemiologia. Recife, PE, Brasil.Mycobacterium tuberculosis (Mtb) has acquired resistance and consequently the antibiotic therapeutic options available against this microorganism are limited. In this scenario, the use of usnic acid (UA), a natural compound, encapsulated into liposomes is proposed as a new approach in multidrug-resistant tuberculosis (MDR-TB) therapy. Thus the aim of this study was to evaluate the effect of the encapsulation of UA into liposomes, as well as its combination with antituberculous agents such as rifampicin (RIF) and isoniazid (INH) against MDR-TB clinical isolates. The in vitro antimycobacterial activity of UA-loaded liposomes (UA-Lipo) against MDR-TB was assessed by the microdilution method. The in vitro interaction of UA with antituberculous agents was carried out using checkerboard method. Minimal inhibitory concentration values were 31.25 and 0.98 µg/mL for UA and UA-Lipo, respectively. The results exhibited a synergistic interaction between RIF and UA [fractional inhibitory concentration index (FICI) = 0.31] or UA-Lipo (FICI = 0.28). Regarding INH, the combination of UA or UA-Lipo revealed no marked effect (FICI = 1.30-2.50). The UA-Lipo may be used as a dosage form to improve the antimycobacterial activity of RIF, a first-line drug for the treatment of infections caused by Mtb.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19653/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27143488 2016 car-eff.oa.pdf27143488 2016 car-eff.oa.pdfapplication/pdf255361https://www.arca.fiocruz.br/bitstream/icict/19653/2/27143488%202016%20car-eff.oa.pdfb3681ed6828f8288c0322fa442518518MD52TEXT27143488 2016 car-eff.oa.pdf.txt27143488 2016 car-eff.oa.pdf.txtExtracted texttext/plain24688https://www.arca.fiocruz.br/bitstream/icict/19653/3/27143488%202016%20car-eff.oa.pdf.txtbdf1a9da728f848073f2660b379ad369MD53icict/196532019-04-02 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| dc.title.pt_BR.fl_str_mv |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| title |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| spellingShingle |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates Ferraz-Carvalho, Rafaela S. MDR-TB Atividade antimicobacteriana Sinergismo Ácido usnic Lipossomas MDR-TB Antimycobacterial activity Synergism Usnic acid Liposomes Mycobacterium tuberculosis / Efeitos de drogas Rifampicina / Farmacologia Antibióticos Antituberculosos / Farmacologia Benzofuranos / Farmacologia Isoniazida / Farmacologia Lipossomas |
| title_short |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| title_full |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| title_fullStr |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| title_full_unstemmed |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| title_sort |
Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates |
| author |
Ferraz-Carvalho, Rafaela S. |
| author_facet |
Ferraz-Carvalho, Rafaela S. Pereira, Marcela A. Linhares, Leonardo A. Lira-Nogueira, Mariane C. Cavalcanti, Isabella Mf Santos-Magalhães, Nereide S. Montenegro, Lílian M. |
| author_role |
author |
| author2 |
Pereira, Marcela A. Linhares, Leonardo A. Lira-Nogueira, Mariane C. Cavalcanti, Isabella Mf Santos-Magalhães, Nereide S. Montenegro, Lílian M. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferraz-Carvalho, Rafaela S. Pereira, Marcela A. Linhares, Leonardo A. Lira-Nogueira, Mariane C. Cavalcanti, Isabella Mf Santos-Magalhães, Nereide S. Montenegro, Lílian M. |
| dc.subject.other.none.fl_str_mv |
MDR-TB Atividade antimicobacteriana Sinergismo Ácido usnic Lipossomas |
| topic |
MDR-TB Atividade antimicobacteriana Sinergismo Ácido usnic Lipossomas MDR-TB Antimycobacterial activity Synergism Usnic acid Liposomes Mycobacterium tuberculosis / Efeitos de drogas Rifampicina / Farmacologia Antibióticos Antituberculosos / Farmacologia Benzofuranos / Farmacologia Isoniazida / Farmacologia Lipossomas |
| dc.subject.en.none.fl_str_mv |
MDR-TB Antimycobacterial activity Synergism Usnic acid Liposomes |
| dc.subject.decs.none.fl_str_mv |
Mycobacterium tuberculosis / Efeitos de drogas Rifampicina / Farmacologia Antibióticos Antituberculosos / Farmacologia Benzofuranos / Farmacologia Isoniazida / Farmacologia Lipossomas |
| description |
Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo-Asami. Recife, PE, Brasil. |
| publishDate |
2016 |
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2016 |
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2017-06-28T19:08:16Z |
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2017-06-28T19:08:16Z |
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info:eu-repo/semantics/article |
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FERRAZ-CARVALHO, R. S. et al. Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 5, p. 330–334, May 2016. |
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https://www.arca.fiocruz.br/handle/icict/19653 |
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1678-8060 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/0074-02760150454 |
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FERRAZ-CARVALHO, R. S. et al. Effects of the encapsulation of usnic acid into liposomes and interactions with antituberculous agents against multidrug-resistant tuberculosis clinical isolates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 5, p. 330–334, May 2016. 1678-8060 10.1590/0074-02760150454 |
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