Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA

Detalhes bibliográficos
Autor(a) principal: Souza, Carolina Senra Alves de
Data de Publicação: 2018
Outros Autores: Gomes, Luciana Inácia, Siqueira, Liliane Maria Vidal, Coelho, Paulo Marcos Zech, Rabello, Ana Lúcia Teles, Oliveira, Edward José de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55090
Resumo: Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
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spelling Souza, Carolina Senra Alves deGomes, Luciana InáciaSiqueira, Liliane Maria VidalCoelho, Paulo Marcos ZechRabello, Ana Lúcia TelesOliveira, Edward José de2022-10-10T18:38:49Z2022-10-10T18:38:49Z2018SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7.1756-0500https://www.arca.fiocruz.br/handle/icict/5509010.1186/s13104-018-3571-7engBiomed CentralDevelopment of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleClinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Objective: We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area. Results: The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.DiagnosisDisease controlPCR-ELISASchistosoma mansoniSchistosomiasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDevelopment of a laboratorial platform for diagnosis.pdfDevelopment of a laboratorial platform for diagnosis.pdfapplication/pdf997432https://www.arca.fiocruz.br/bitstream/icict/55090/2/Development%20of%20a%20laboratorial%20platform%20for%20diagnosis.pdf281e5a5640bbccc772e62662c03fe11dMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/55090/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/550902022-10-10 15:38:51.067oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-10-10T18:38:51Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
title Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
spellingShingle Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
Souza, Carolina Senra Alves de
Diagnosis
Disease control
PCR-ELISA
Schistosoma mansoni
Schistosomiasis
title_short Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
title_full Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
title_fullStr Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
title_full_unstemmed Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
title_sort Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
author Souza, Carolina Senra Alves de
author_facet Souza, Carolina Senra Alves de
Gomes, Luciana Inácia
Siqueira, Liliane Maria Vidal
Coelho, Paulo Marcos Zech
Rabello, Ana Lúcia Teles
Oliveira, Edward José de
author_role author
author2 Gomes, Luciana Inácia
Siqueira, Liliane Maria Vidal
Coelho, Paulo Marcos Zech
Rabello, Ana Lúcia Teles
Oliveira, Edward José de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Carolina Senra Alves de
Gomes, Luciana Inácia
Siqueira, Liliane Maria Vidal
Coelho, Paulo Marcos Zech
Rabello, Ana Lúcia Teles
Oliveira, Edward José de
dc.subject.en.en_US.fl_str_mv Diagnosis
Disease control
PCR-ELISA
Schistosoma mansoni
Schistosomiasis
topic Diagnosis
Disease control
PCR-ELISA
Schistosoma mansoni
Schistosomiasis
description Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2022-10-10T18:38:49Z
dc.date.available.fl_str_mv 2022-10-10T18:38:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55090
dc.identifier.issn.en_US.fl_str_mv 1756-0500
dc.identifier.doi.none.fl_str_mv 10.1186/s13104-018-3571-7
identifier_str_mv SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7.
1756-0500
10.1186/s13104-018-3571-7
url https://www.arca.fiocruz.br/handle/icict/55090
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55090/2/Development%20of%20a%20laboratorial%20platform%20for%20diagnosis.pdf
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