Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro

Detalhes bibliográficos
Autor(a) principal: Luz, Mauricio R. M. P.
Data de Publicação: 1993
Outros Autores: Soeiro, Maria de Nazaré C., Araujo-Jorge, Tania C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43581
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Luz, Mauricio R. M. P.Soeiro, Maria de Nazaré C.Araujo-Jorge, Tania C.2020-09-23T18:48:01Z2020-09-23T18:48:01Z1993LUZ, Mauricio R. M. P.; SOEIRO, Maria de Nazaré C.; ARAUJO-JORGE, Tania C. Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro. Memórias do Instituto Oswaldo Cruz, Rio de janeiro, v. 88, n. 2, p. 235-241, Apr./June 1993.0074-0276https://www.arca.fiocruz.br/handle/icict/4358110.1590/S0074-027619930002000101678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziELISAInfecção in vitroTrypanosoma cruziELISAInfection in vitroImproved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.We herein present an improved assay for detecting the presence of Trypanosoma cruzi in infected cultures. Using chagasic human sera (CHS), we were able to detect T. cruzi infection in primary cultures of both peritoneal macrophages and heart muscle cells (MHC). To avoid elevated background levels - hitherto observed in all experiments especially in those using HMC - CHS were preincubated with uninfected cells in monolayers or suspensions prior to being used for detection of T. cruzi in infected monolayers. Preincubation with cell suspensions gave better results than with monolayers, reducing background by up to three times and increasing sensitivity by to twenty times. In addition, the continous fibroplastic cell line L929 was shown to be suitable for preadsorption of CHS. These results indicate that the high background levels observed in previous reports may be due to the presence of human autoantibodies that recognize surface and/or extracellular matrix components in cell monolayers. We therefore propose a modified procedure that increases the performance of the ELISA method, making it an useful tool even in cultures that would otherwise be expected to present low levels of infection or high levels of background.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43581/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaNSoeiro_TaniaCAJorge_IOC_1993.pdfMariaNSoeiro_TaniaCAJorge_IOC_1993.pdfapplication/pdf375959https://www.arca.fiocruz.br/bitstream/icict/43581/2/MariaNSoeiro_TaniaCAJorge_IOC_1993.pdfc2616ca4e976e14d81f46ae2486cbb54MD52TEXTMariaNSoeiro_TaniaCAJorge_IOC_1993.pdf.txtMariaNSoeiro_TaniaCAJorge_IOC_1993.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
title Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
spellingShingle Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
Luz, Mauricio R. M. P.
Trypanosoma cruzi
ELISA
Infecção in vitro
Trypanosoma cruzi
ELISA
Infection in vitro
title_short Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
title_full Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
title_fullStr Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
title_full_unstemmed Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
title_sort Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro
author Luz, Mauricio R. M. P.
author_facet Luz, Mauricio R. M. P.
Soeiro, Maria de Nazaré C.
Araujo-Jorge, Tania C.
author_role author
author2 Soeiro, Maria de Nazaré C.
Araujo-Jorge, Tania C.
author2_role author
author
dc.contributor.author.fl_str_mv Luz, Mauricio R. M. P.
Soeiro, Maria de Nazaré C.
Araujo-Jorge, Tania C.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
ELISA
Infecção in vitro
topic Trypanosoma cruzi
ELISA
Infecção in vitro
Trypanosoma cruzi
ELISA
Infection in vitro
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
ELISA
Infection in vitro
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 1993
dc.date.issued.fl_str_mv 1993
dc.date.accessioned.fl_str_mv 2020-09-23T18:48:01Z
dc.date.available.fl_str_mv 2020-09-23T18:48:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LUZ, Mauricio R. M. P.; SOEIRO, Maria de Nazaré C.; ARAUJO-JORGE, Tania C. Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro. Memórias do Instituto Oswaldo Cruz, Rio de janeiro, v. 88, n. 2, p. 235-241, Apr./June 1993.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43581
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761993000200010
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv LUZ, Mauricio R. M. P.; SOEIRO, Maria de Nazaré C.; ARAUJO-JORGE, Tania C. Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro. Memórias do Instituto Oswaldo Cruz, Rio de janeiro, v. 88, n. 2, p. 235-241, Apr./June 1993.
0074-0276
10.1590/S0074-02761993000200010
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43581
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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