DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27201 |
Resumo: | Silva, Carlos Gustavo Régis. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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Reis, Bruno Luiz Fonseca SchamberNardelli, SheilaSilva, Carlos Gustavo RégisCampos, Priscila CarneiroCerqueira, Paula GonçalvesLima, Sabrina AlmeidaFranco, Glória ReginaMacedo, Andrea MaraPena, Sergio Danilo JunhoCazaux, ChristopheHoffmann, Jean-SébastienMotta, Maria Cristina MachadoSchenkman, SergioTeixeira, Santuza Maria RibeiroMachado, Carlos Renato2018-06-29T16:24:58Z2018-06-29T16:24:58Z2012REIS, B. L. F. S. et al. DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions. Molecular and Biochemical Parasitology, v. 183, p. 122– 131, 2012.0166-6851https://www.arca.fiocruz.br/handle/icict/2720110.1016/j.molbiopara.2012.02.007Silva, Carlos Gustavo Régis. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.CNPq-Brazil (MCT/CNPq/MSSCTIE-DECIT 25/2006-Estudo de Doenc¸ as Negligenciadas), PRONEX and FAPEMIG.Federal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil / School of Medical Sciences. Superior Learning and Development Center. Campina Grande, PB, BrazilFederal University of São Paulo. Microbiology, Immunology and Parasitology Department. São Paulo, SP, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilUniversity of Toulouse. Institute of Pharmacology and Structural Biology. Toulouse, FranceUniversity of Toulouse. Institute of Pharmacology and Structural Biology. Toulouse, FranceFederal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Hertha Meyer Cellular Ultra structure Laboratory. Rio de Janeiro, RJ, BrazilFederal University of São Paulo. Microbiology, Immunology and Parasitology Department. São Paulo, SP, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Biochemistry and Immunology. Belo Horizonte, MG, BrazilSpecific DNA repair pathways from Trypanosoma cruzi are believed to protect genomic DNA and kinetoplast DNA (kDNA) from mutations. Particular pathways are supposed to operate in order to repair nucleotides oxidized by reactive oxygen species (ROS) during parasite infection, being 7,8-dihydro-8-oxoguanine (8oxoG) a frequent and highly mutagenic base alteration. If unrepaired, 8oxoG can lead to cytotoxic base transversions during DNA replication. In mammals, DNA polymerase beta (Polβ) is mainly involved in base excision repair (BER) of oxidative damage. However its biological role in T. cruzi is still unknown. We show, by immunofluorescence localization, that T. cruzi DNA polymerase beta (Tcpolβ) is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes. Nevertheless, this polymerase was detected inside the mitochondrial matrix of trypomastigote forms, which are not able to replicate in culture. Parasites over expressing Tcpolβ showed reduced levels of 8oxoG in kDNA and an increased survival after treatment with hydrogen peroxide when compared to control cells. However, this resistance was lost after treating Tcpolβ overexpressors with methoxiamine, a potent BER inhibitor. Curiously, a presumed DNA repair focus containing Tcpolβ was identified in the vicinity of kDNA of cultured wild type epimastigotes after treatment with hydrogen peroxide. Taken together our data suggest participation of Tcpolβ during kDNA replication and repair of oxidative DNA damage induced by genotoxic stress in this organelle.engElsevierReparo de DNATrypanosoma cruziEstresse oxidativoOxoguanineKinetoplastDNA repairTrypanosoma cruziOxidative stressOxoguanineKinetoplastDNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27201/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSchamber- Reis DNA polymerase beta from Trypanosoma ....pdfSchamber- Reis DNA polymerase beta from Trypanosoma 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| dc.title.pt_BR.fl_str_mv |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| title |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| spellingShingle |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions Reis, Bruno Luiz Fonseca Schamber Reparo de DNA Trypanosoma cruzi Estresse oxidativo Oxoguanine Kinetoplast DNA repair Trypanosoma cruzi Oxidative stress Oxoguanine Kinetoplast |
| title_short |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| title_full |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| title_fullStr |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| title_full_unstemmed |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| title_sort |
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions |
| author |
Reis, Bruno Luiz Fonseca Schamber |
| author_facet |
Reis, Bruno Luiz Fonseca Schamber Nardelli, Sheila Silva, Carlos Gustavo Régis Campos, Priscila Carneiro Cerqueira, Paula Gonçalves Lima, Sabrina Almeida Franco, Glória Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Cazaux, Christophe Hoffmann, Jean-Sébastien Motta, Maria Cristina Machado Schenkman, Sergio Teixeira, Santuza Maria Ribeiro Machado, Carlos Renato |
| author_role |
author |
| author2 |
Nardelli, Sheila Silva, Carlos Gustavo Régis Campos, Priscila Carneiro Cerqueira, Paula Gonçalves Lima, Sabrina Almeida Franco, Glória Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Cazaux, Christophe Hoffmann, Jean-Sébastien Motta, Maria Cristina Machado Schenkman, Sergio Teixeira, Santuza Maria Ribeiro Machado, Carlos Renato |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Reis, Bruno Luiz Fonseca Schamber Nardelli, Sheila Silva, Carlos Gustavo Régis Campos, Priscila Carneiro Cerqueira, Paula Gonçalves Lima, Sabrina Almeida Franco, Glória Regina Macedo, Andrea Mara Pena, Sergio Danilo Junho Cazaux, Christophe Hoffmann, Jean-Sébastien Motta, Maria Cristina Machado Schenkman, Sergio Teixeira, Santuza Maria Ribeiro Machado, Carlos Renato |
| dc.subject.other.pt_BR.fl_str_mv |
Reparo de DNA Trypanosoma cruzi Estresse oxidativo Oxoguanine Kinetoplast |
| topic |
Reparo de DNA Trypanosoma cruzi Estresse oxidativo Oxoguanine Kinetoplast DNA repair Trypanosoma cruzi Oxidative stress Oxoguanine Kinetoplast |
| dc.subject.en.pt_BR.fl_str_mv |
DNA repair Trypanosoma cruzi Oxidative stress Oxoguanine Kinetoplast |
| description |
Silva, Carlos Gustavo Régis. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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2012 |
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2012 |
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2018-06-29T16:24:58Z |
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2018-06-29T16:24:58Z |
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info:eu-repo/semantics/article |
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REIS, B. L. F. S. et al. DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions. Molecular and Biochemical Parasitology, v. 183, p. 122– 131, 2012. |
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https://www.arca.fiocruz.br/handle/icict/27201 |
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0166-6851 |
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10.1016/j.molbiopara.2012.02.007 |
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REIS, B. L. F. S. et al. DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions. Molecular and Biochemical Parasitology, v. 183, p. 122– 131, 2012. 0166-6851 10.1016/j.molbiopara.2012.02.007 |
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