Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7837 |
Resumo: | FAPEMIG, CNPq, CPqRR-FIOCRUZ |
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Quaresma, Patrícia FláviaRamos, Mariana Campos das Neves FarahCarvalho, Gustavo Mayr de LimaAndrade Filho, José Dilermando2014-06-24T18:32:22Z2014-06-24T18:32:22Z2012QUARESMA, Patrícia Flávia et al. Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 480-4850074-0276https://www.arca.fiocruz.br/handle/icict/7837FAPEMIG, CNPq, CPqRR-FIOCRUZFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, BrasilLeishmania spp are distributed throughout the world and different species are associated with varying degrees of disease severity. However, leishmaniasis is thought to be confined to areas of the world where its insect vectors, sandflies, are present. Phlebotomine sandflies obtain blood meals from a variety of wild and domestic animals and sometimes from humans. These vectors transmit Leishmania spp, the aetiological agent of leishmaniasis. Identification of sandfly blood meals has generally been performed using serological methods, although a few studies have used molecular procedures in artificially fed insects. In this study, cytochrome b gene (cytB) polymerase chain reaction (PCR) was performed in DNA samples isolated from 38 engorged Psychodopygus lloydi and the expected 359 bp fragment was identified from all of the samples. The amplified product was digested using restriction enzymes and analysed for restriction fragment length polymorphisms (RFLPs). We identified food sources for 23 females; 34.8% yielded a primate-specific banding profile and 26.1% and 39.1% showed banding patterns specific to birds or mixed restriction profiles (rodent/marsupial, human/bird, rodent/marsupial/human), respectively. The food sources of 15 flies could not be identified. Two female P. lloydi were determined to be infected by Leishmania using internal transcribed spacer 1 and heat shock protein 70 kDa PCR-RFLP. The two female sandflies, both of which fed on rodents/marsupials, were further characterised as infected with Leishmania (Viannia) braziliensis. These results constitute an important step towards applying methodologies based on cytB amplification as a tool for identifying the food sources of female sandflies.engFundação Oswaldo CruzNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlephlebotominesfood source identificationcytB PCRLeishmania natural infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdfNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdfapplication/pdf324232https://www.arca.fiocruz.br/bitstream/icict/7837/1/Natural%20Leishmania%20sp.%20reservoirs%20and%20phlebotomine%20sandfly%20food%20source%20identification%20in%20the%20Ibitipoca%20State%20Park%2c%20Minas%20Gerais%2c%20Brazil.pdfa8c352566ec53e6c15171099e6016e2eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7837/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdf.txtNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdf.txtExtracted texttext/plain33441https://www.arca.fiocruz.br/bitstream/icict/7837/5/Natural%20Leishmania%20sp.%20reservoirs%20and%20phlebotomine%20sandfly%20food%20source%20identification%20in%20the%20Ibitipoca%20State%20Park%2c%20Minas%20Gerais%2c%20Brazil.pdf.txt40b451ae6292addeeab2e7e8d50830d4MD55THUMBNAILNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdf.jpgNatural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil.pdf.jpgGenerated Thumbnailimage/jpeg1862https://www.arca.fiocruz.br/bitstream/icict/7837/4/Natural%20Leishmania%20sp.%20reservoirs%20and%20phlebotomine%20sandfly%20food%20source%20identification%20in%20the%20Ibitipoca%20State%20Park%2c%20Minas%20Gerais%2c%20Brazil.pdf.jpg787482c17bd5e09dcf68aabac291e4e4MD54icict/78372018-04-05 23:45:32.336oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T02:45:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| title |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| spellingShingle |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil Quaresma, Patrícia Flávia phlebotomines food source identification cytB PCR Leishmania natural infection |
| title_short |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| title_full |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| title_fullStr |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| title_full_unstemmed |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| title_sort |
Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil |
| author |
Quaresma, Patrícia Flávia |
| author_facet |
Quaresma, Patrícia Flávia Ramos, Mariana Campos das Neves Farah Carvalho, Gustavo Mayr de Lima Andrade Filho, José Dilermando |
| author_role |
author |
| author2 |
Ramos, Mariana Campos das Neves Farah Carvalho, Gustavo Mayr de Lima Andrade Filho, José Dilermando |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Quaresma, Patrícia Flávia Ramos, Mariana Campos das Neves Farah Carvalho, Gustavo Mayr de Lima Andrade Filho, José Dilermando |
| dc.subject.en.pt_BR.fl_str_mv |
phlebotomines food source identification cytB PCR Leishmania natural infection |
| topic |
phlebotomines food source identification cytB PCR Leishmania natural infection |
| description |
FAPEMIG, CNPq, CPqRR-FIOCRUZ |
| publishDate |
2012 |
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2012 |
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2014-06-24T18:32:22Z |
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2014-06-24T18:32:22Z |
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info:eu-repo/semantics/publishedVersion |
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QUARESMA, Patrícia Flávia et al. Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 480-485 |
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https://www.arca.fiocruz.br/handle/icict/7837 |
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0074-0276 |
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QUARESMA, Patrícia Flávia et al. Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in the Ibitipoca State Park, Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 480-485 0074-0276 |
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eng |
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Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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