Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2002 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44194 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de Micobactérias. Rio de Janeiro, RJ, Brasil. |
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Ferreira, Rosa Maria CarvalhoSaad, Maria Helena FéresSilva, Marlei Gomes daFonseca, Leila de Souza2020-10-26T14:20:04Z2020-10-26T14:20:04Z2002FERREIRA, Rosa Maria Carvalho et al. Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 5, p. 725-729, July 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4419410.1590/S0074-027620020005000241678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.TuberculoseMicobactérias não tuberculosasAcquired immunodeficiencyIdentificação bioquímicaRio de JaneiroBrasilNon-tuberculous mycobacteriaTuberculosisAcquired immunodeficiencyIS1245Biochemical identificationRio de JaneiroBrazilNon-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Erainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.The aim of this study was to determine the prevalence of non-tuberculous mycobacteria (NTM) isolates at University Hospital, Reference Center for Aids in Rio de Janeiro, Brazil, during one year. We used standard biochemical tests for species identification and IS1245 PCR amplification was applied as a Mycobacterium avium specific identification marker. Four hundred and four specimens from 233 patients yielded acid-fast bacilli growth. M. tuberculosis was identified in 85% of the patients and NTM in 15%. NTM disseminated infection was a common event correlated with human immunodeficiency virus (HIV) infected patients and only in HIV negative patients the source of NTM was non sterile site. M. avium complex (MAC) was biochemically identified in 57.8% (49/83) of NTM isolates, most of them from sterile sites (75.5%), and in 94% (46/49) the IS 1245 marker specific for M. avium was present. Twenty NTM strains showed a MAC biochemical pattern with the exception of a urease-positive (99% of MAC are urease-negative), however IS1245 was detected in 96% of the strains leading to their identification as M. avium. In this group differences in NTM source was not significant. The second most frequently isolated NTM was identified as M. scrofulaceum (7.2%), followed by M. terrae (3.6%), M. gordonae (2.4%), M. chelonae (1.2%), M. fortuitum (1.2%) and one strain which could not be identified. All were IS1245 negative except for one strain identified as M. scrofulaceum. It is interesting to note that non-sterile sites were the major source of these isolates (92.8%). Our finding indicated that M. avium is still the major atypical species among in the MAC isolates recovered from Brazilian Aids patients without highty active antiretroviral therapy schema. Some discrepancies were seen between the identification methods and further investigations must be done to better characterize NTM isolates using other phenotypic and genotypic methods.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44194/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaHelena_FeresSaad_etal_IOC_2002.pdfMariaHelena_FeresSaad_etal_IOC_2002.pdfapplication/pdf50575https://www.arca.fiocruz.br/bitstream/icict/44194/2/MariaHelena_FeresSaad_etal_IOC_2002.pdf193256c16cd4b5bade7bde33de7ec10aMD52TEXTMariaHelena_FeresSaad_etal_IOC_2002.pdf.txtMariaHelena_FeresSaad_etal_IOC_2002.pdf.txtExtracted texttext/plain24851https://www.arca.fiocruz.br/bitstream/icict/44194/3/MariaHelena_FeresSaad_etal_IOC_2002.pdf.txt809c352c305bfce812845b8f85406b8eMD53icict/441942020-10-27 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Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| title |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| spellingShingle |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era Ferreira, Rosa Maria Carvalho Tuberculose Micobactérias não tuberculosas Acquired immunodeficiency Identificação bioquímica Rio de Janeiro Brasil Non-tuberculous mycobacteria Tuberculosis Acquired immunodeficiency IS1245 Biochemical identification Rio de Janeiro Brazil |
| title_short |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| title_full |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| title_fullStr |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| title_full_unstemmed |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| title_sort |
Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era |
| author |
Ferreira, Rosa Maria Carvalho |
| author_facet |
Ferreira, Rosa Maria Carvalho Saad, Maria Helena Féres Silva, Marlei Gomes da Fonseca, Leila de Souza |
| author_role |
author |
| author2 |
Saad, Maria Helena Féres Silva, Marlei Gomes da Fonseca, Leila de Souza |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Rosa Maria Carvalho Saad, Maria Helena Féres Silva, Marlei Gomes da Fonseca, Leila de Souza |
| dc.subject.other.pt_BR.fl_str_mv |
Tuberculose Micobactérias não tuberculosas Acquired immunodeficiency Identificação bioquímica Rio de Janeiro Brasil |
| topic |
Tuberculose Micobactérias não tuberculosas Acquired immunodeficiency Identificação bioquímica Rio de Janeiro Brasil Non-tuberculous mycobacteria Tuberculosis Acquired immunodeficiency IS1245 Biochemical identification Rio de Janeiro Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Non-tuberculous mycobacteria Tuberculosis Acquired immunodeficiency IS1245 Biochemical identification Rio de Janeiro Brazil |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de Micobactérias. Rio de Janeiro, RJ, Brasil. |
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2002 |
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2002 |
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2020-10-26T14:20:04Z |
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2020-10-26T14:20:04Z |
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FERREIRA, Rosa Maria Carvalho et al. Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 5, p. 725-729, July 2002. |
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https://www.arca.fiocruz.br/handle/icict/44194 |
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0074-0276 |
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10.1590/S0074-02762002000500024 |
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1678-8060 |
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FERREIRA, Rosa Maria Carvalho et al. Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 5, p. 725-729, July 2002. 0074-0276 10.1590/S0074-02762002000500024 1678-8060 |
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