PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Guilherme Marx de
Data de Publicação: 2015
Outros Autores: Madeira, Maria de Fatima, Oliveira, Fernanda S., Pacheco, Raquel S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35088
Resumo: Maria de Fátima Madeira. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
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spelling Oliveira, Guilherme Marx deMadeira, Maria de FatimaOliveira, Fernanda S.Pacheco, Raquel S.2019-08-27T12:56:50Z2019-08-27T12:56:50Z2015OLIVEIRA, Guilherme Marx de et al. PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis. Journal of Parasitology, v. 101, n. 1, p. 91-93, 2015.0022-3395https://www.arca.fiocruz.br/handle/icict/3508810.1645/14-567.11937-2345Maria de Fátima Madeira. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.2020-08-27Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Tegumentary leishmaniasis (TL) is a zoonotic disease that affects humans and domestic dogs. In Brazil, TL is considered endemic, and Leishmania (Viannia) braziliensis is the prevalent species causing this disease. There is debate about the role of dogs (Canis familiaris) as domestic reservoirs in the transmission cycle of TL. To date, classical parasitological techniques, including parasite isolation in culture media, have been able to detect parasites only from cutaneous lesions. In this study, we detected L. (V.) braziliensis DNA in intact skin fragments collected from 3 naturally infected dogs from the state of Rio de Janeiro, Brazil, with the use of PCR techniques associated with molecular hybridization. The detection of parasitic DNA in this anatomical site is an important finding visa` -vis the importance of the domestic dogs in endemic areas of TL.engAmerican Society of ParasitologistsPCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTegumentary leishmaniasisLeishmania (Viannia) braziliensisDogsPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/35088/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Oliveira_Guilherme_etal_INI_2105.pdfve_Oliveira_Guilherme_etal_INI_2105.pdfapplication/pdf250279https://www.arca.fiocruz.br/bitstream/icict/35088/2/ve_Oliveira_Guilherme_etal_INI_2105.pdf2b2d6f264116bd52eda445435572398cMD52TEXTve_Oliveira_Guilherme_etal_INI_2105.pdf.txtve_Oliveira_Guilherme_etal_INI_2105.pdf.txtExtracted texttext/plain20315https://www.arca.fiocruz.br/bitstream/icict/35088/3/ve_Oliveira_Guilherme_etal_INI_2105.pdf.txta9b1a167db57be18e7b13ae09ae2b08aMD53icict/350882021-03-24 16:32:35.811oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
title PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
spellingShingle PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
Oliveira, Guilherme Marx de
Tegumentary leishmaniasis
Leishmania (Viannia) braziliensis
Dogs
PCR
title_short PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
title_full PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
title_fullStr PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
title_full_unstemmed PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
title_sort PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis
author Oliveira, Guilherme Marx de
author_facet Oliveira, Guilherme Marx de
Madeira, Maria de Fatima
Oliveira, Fernanda S.
Pacheco, Raquel S.
author_role author
author2 Madeira, Maria de Fatima
Oliveira, Fernanda S.
Pacheco, Raquel S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Guilherme Marx de
Madeira, Maria de Fatima
Oliveira, Fernanda S.
Pacheco, Raquel S.
dc.subject.en.pt_BR.fl_str_mv Tegumentary leishmaniasis
Leishmania (Viannia) braziliensis
Dogs
PCR
topic Tegumentary leishmaniasis
Leishmania (Viannia) braziliensis
Dogs
PCR
description Maria de Fátima Madeira. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2019-08-27T12:56:50Z
dc.date.available.fl_str_mv 2019-08-27T12:56:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Guilherme Marx de et al. PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis. Journal of Parasitology, v. 101, n. 1, p. 91-93, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35088
dc.identifier.issn.pt_BR.fl_str_mv 0022-3395
dc.identifier.doi.none.fl_str_mv 10.1645/14-567.1
dc.identifier.eissn.none.fl_str_mv 1937-2345
identifier_str_mv OLIVEIRA, Guilherme Marx de et al. PCR associated with molecular hybridization detects Leishmania (Viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis. Journal of Parasitology, v. 101, n. 1, p. 91-93, 2015.
0022-3395
10.1645/14-567.1
1937-2345
url https://www.arca.fiocruz.br/handle/icict/35088
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Parasitologists
publisher.none.fl_str_mv American Society of Parasitologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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