A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology

Detalhes bibliográficos
Autor(a) principal: Silva, Elisângela Fátima
Data de Publicação: 2012
Outros Autores: Orsi, Mariana, Andrade, Angela Leão, Domingues, Rosana Zacarias, Silva, Breno de Mello, Araújo, Helena Rocha Corrêa de, Pimenta, Paulo Filemon Paolucci, Diamond, Michael S., Rocha, Eliseu Soares de Oliveira, Kroon, Erna Geessien, Malaquias, Luiz Cosme Cotta, Coelho, Luiz Felipe Leomil
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7945
Resumo: FAPEMIG and CNPq
id CRUZ_4be63fdd395f4d6d1f0d223df0603274
oai_identifier_str oai:www.arca.fiocruz.br:icict/7945
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Silva, Elisângela FátimaOrsi, MarianaAndrade, Angela LeãoDomingues, Rosana ZacariasSilva, Breno de MelloAraújo, Helena Rocha Corrêa dePimenta, Paulo Filemon PaolucciDiamond, Michael S.Rocha, Eliseu Soares de OliveiraKroon, Erna GeessienMalaquias, Luiz Cosme CottaCoelho, Luiz Felipe Leomil2014-07-14T13:53:42Z2014-07-14T13:53:42Z2012SILVA, Elisângela Fátima et al. A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology. 2012, vol.10, pp. 13https://www.arca.fiocruz.br/handle/icict/794510.1186/1477-3155-10-13FAPEMIG and CNPqUniversidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil.Universidade Federal de Ouro Preto. Departamento de Química. Ouro Preto, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Química. Belo Horizonte, MG, Brazil.Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil.Washington University School of Medicine. Departments of Medicine, Molecular Microbiology, Pathology & Immunology. Saint Louis, Missouri, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Laboratório de Vírus. Belo Horizonte, MG, Brazil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Alfenas, MG, Brazil.Background: Dengue is a major public health problem worldwide, especially in the tropical and subtropical regions of the world. Infection with a single Dengue virus (DENV) serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients experiencing secondary infection with a different serotype progresses to the severe form of the disease, dengue hemorrhagic fever/dengue shock syndrome. Currently, there are no licensed vaccines or antiviral drugs to prevent or treat dengue infections. Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines. Findings: Here, we used a murine model to evaluate the IgG production after administration of inactivated DENV corresponding to all four serotypes adsorbed to bovine serum albumin nanoparticles. This formulation induced a production of anti-DENV IgG antibodies (p < 0.001). However, plaque reduction neutralization assays with the four DENV serotypes revealed that these antibodies have no neutralizing activity in the dilutions tested. Conclusions: Our results show that while the nanoparticle system induces humoral responses against DENV, further investigation with different DENV antigens will be required to improve immunogenicity, epitope specicity, and functional activity to make this platform a viable option for DENV vaccines.engBioMed CentralA tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinactivated Dengue vírusNanoparticleshumoral responseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALA tetravalent dengue nanoparticle stimulates antibody production in mice.pdfA tetravalent dengue nanoparticle stimulates antibody production in mice.pdfapplication/pdf1236821https://www.arca.fiocruz.br/bitstream/icict/7945/1/A%20tetravalent%20dengue%20nanoparticle%20stimulates%20antibody%20production%20in%20mice.pdf9835d5dc4e0057f58331889a3e79ef7aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7945/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTA tetravalent dengue nanoparticle stimulates antibody production in mice.pdf.txtA tetravalent dengue nanoparticle stimulates antibody production in mice.pdf.txtExtracted texttext/plain5https://www.arca.fiocruz.br/bitstream/icict/7945/3/A%20tetravalent%20dengue%20nanoparticle%20stimulates%20antibody%20production%20in%20mice.pdf.txtcc9067c2ee470dc248b14b194209a34eMD53icict/79452018-04-06 08:43:53.015oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
title A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
spellingShingle A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
Silva, Elisângela Fátima
inactivated Dengue vírus
Nanoparticles
humoral response
title_short A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
title_full A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
title_fullStr A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
title_full_unstemmed A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
title_sort A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology
author Silva, Elisângela Fátima
author_facet Silva, Elisângela Fátima
Orsi, Mariana
Andrade, Angela Leão
Domingues, Rosana Zacarias
Silva, Breno de Mello
Araújo, Helena Rocha Corrêa de
Pimenta, Paulo Filemon Paolucci
Diamond, Michael S.
Rocha, Eliseu Soares de Oliveira
Kroon, Erna Geessien
Malaquias, Luiz Cosme Cotta
Coelho, Luiz Felipe Leomil
author_role author
author2 Orsi, Mariana
Andrade, Angela Leão
Domingues, Rosana Zacarias
Silva, Breno de Mello
Araújo, Helena Rocha Corrêa de
Pimenta, Paulo Filemon Paolucci
Diamond, Michael S.
Rocha, Eliseu Soares de Oliveira
Kroon, Erna Geessien
Malaquias, Luiz Cosme Cotta
Coelho, Luiz Felipe Leomil
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Elisângela Fátima
Orsi, Mariana
Andrade, Angela Leão
Domingues, Rosana Zacarias
Silva, Breno de Mello
Araújo, Helena Rocha Corrêa de
Pimenta, Paulo Filemon Paolucci
Diamond, Michael S.
Rocha, Eliseu Soares de Oliveira
Kroon, Erna Geessien
Malaquias, Luiz Cosme Cotta
Coelho, Luiz Felipe Leomil
dc.subject.en.pt_BR.fl_str_mv inactivated Dengue vírus
Nanoparticles
humoral response
topic inactivated Dengue vírus
Nanoparticles
humoral response
description FAPEMIG and CNPq
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-07-14T13:53:42Z
dc.date.available.fl_str_mv 2014-07-14T13:53:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Elisângela Fátima et al. A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology. 2012, vol.10, pp. 13
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7945
dc.identifier.doi.none.fl_str_mv 10.1186/1477-3155-10-13
identifier_str_mv SILVA, Elisângela Fátima et al. A tetravalent dengue nanoparticle stimulates antibody production in mice. Journal of Nanobiotechnology. 2012, vol.10, pp. 13
10.1186/1477-3155-10-13
url https://www.arca.fiocruz.br/handle/icict/7945
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7945/1/A%20tetravalent%20dengue%20nanoparticle%20stimulates%20antibody%20production%20in%20mice.pdf
https://www.arca.fiocruz.br/bitstream/icict/7945/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7945/3/A%20tetravalent%20dengue%20nanoparticle%20stimulates%20antibody%20production%20in%20mice.pdf.txt
bitstream.checksum.fl_str_mv 9835d5dc4e0057f58331889a3e79ef7a
7d48279ffeed55da8dfe2f8e81f3b81f
cc9067c2ee470dc248b14b194209a34e
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009019216855040

Registros relacionados