The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16185 |
Resumo: | CNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France. |
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Abadia, EdgarZhang, JianRitacco, VivianaKremer, KristinRuimy, RaymondRigouts, LeenGomes, Harrison MagdinierElias, Atiná RibeiroFauville-Dufaux, MaryseStoffels, KarolienRasolofo-Razanamparany, VoahangyGarcia de Viedma, DaríoHerranz, MartaAl-Hajoj, SahalRastogi, NalinGarzelli, CarloTortoli, EnricoSuffys, Philip N.van Soolingen, DickRefrégier, GuislaineSola, Christophe2016-10-11T14:01:27Z2016-10-11T14:01:27Z2011ABADIA, Edgar. et al. The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes. BMC Infectious Diseases, v.11, n.110, 8p, 2011.1471-2334https://www.arca.fiocruz.br/handle/icict/1618510.1186/1471-2334-11-110engBioMed CentralMycobacterium tuberculosisGarantia de qualidadeMycobacterium tuberculosismicrobead-based spoligotypingQuality AssuranceThe use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France.CNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France.Instituto Nacional de Enfermedades Infecciosas ANLIS Carlos Malbrán. Buenos Aires, Argentina.National Institute for Public Health and the Environment. Bilthoven, The Netherlands.EA 3964 Université Paris- Diderot & Microbiology Laboratory, Bichat-Claude Bernard Hospital AP-HP. Paris, France..Prince Leopold Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Scientific Institute of Public Health. National Reference Centre of Tuberculosis and Mycobacteria. Brussels, Belgium.Scientific Institute of Public Health. National Reference Centre of Tuberculosis and Mycobacteria. Brussels, Belgium.Institut Pasteur de Madagascar. Unité des Mycobactéries. Antananarivo, Madagascar.Hospital Gregorio Marañón. Servicio de Microbiología Clínica y Enfermedades Infecciosas. Madrid, Spain / CIBER Enfermedades Respiratorias (CIBERES). Spain.Hospital Gregorio Marañón. Servicio de Microbiología Clínica y Enfermedades Infecciosas. Madrid, Spain / CIBER Enfermedades Respiratorias (CIBERES). Spain.King Faisal Specialist Hospital and Research Center. Department of Comparative Medicine. Riyadh, Saudi Arabia.Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory. Abymes, Guadeloupe.Università di Pisa. Dipartimento di Patologia Sperimentale Biotecnologie Mediche Infettivologia ed Epidemiologia. Pisa, Italy.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.National Institute for Public Health and the Environment. Bilthoven, The Netherlands /Radboud University Nijmegen. Department of Pulmonary Diseases and Department of Microbiology. The Netherlands.CNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France.CNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France / Institute Pasteur. Unité de Génétique Mycobactérienne. Paris, France.Background: The classical spoligotyping technique, relying on membrane reverse line-blot hybridization of the spacers of the Mycobacterium tuberculosis CRISPR locus, is used world-wide (598 references in Pubmed on April 8th, 2011). However, until now no inter-laboratory quality control study had been undertaken to validate this technique. We analyzed the quality of membrane-based spoligotyping by comparing it to the recently introduced and highly robust microbead-based spoligotyping. Nine hundred and twenty-seven isolates were analyzed totaling 39,861 data points. Samples were received from 11 international laboratories with a worldwide distribution. Methods: The high-throughput microbead-based Spoligotyping was performed on CTAB and thermolyzate DNA extracted from isolated Mycobacterium tuberculosis complex (MTC) strains coming from the genotyping participating centers. Information regarding how the classical Spoligotyping method was performed by center was available. Genotype discriminatory analyses were carried out by comparing the spoligotypes obtained by both methods. The non parametric U-Mann Whitney homogeneity test and the Spearman rank correlation test were performed to validate the observed results. Results: Seven out of the 11 laboratories (63 %), perfectly typed more than 90% of isolates, 3 scored between 80- 90% and a single center was under 80% reaching 51% concordance only. However, this was mainly due to discordance in a single spacer, likely having a non-functional probe on the membrane used. The centers using thermolyzate DNA performed as well as centers using the more extended CTAB extraction procedure. Few centers shared the same problematic spacers and these problematic spacers were scattered over the whole CRISPR locus (Mostly spacers 15, 14, 18, 37, 39, 40). Conclusions: We confirm that classical spoligotyping is a robust method with generally a high reliability in most centers. The applied DNA extraction procedure (CTAB or thermolyzate) did not affect the results in this study. However performance was center-dependent, suggesting that training is a key component in quality assurance of spoligotyping. Overall, no particular spacer yielded a higher degree of deviating results, suggesting that errors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16185/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALatina_elias_etal_IOC_2011.pdfatina_elias_etal_IOC_2011.pdfapplication/pdf375236https://www.arca.fiocruz.br/bitstream/icict/16185/2/atina_elias_etal_IOC_2011.pdf023728e94d65fe8258bbd6774d3825e4MD52TEXTatina_elias_etal_IOC_2011.pdf.txtatina_elias_etal_IOC_2011.pdf.txtExtracted texttext/plain38716https://www.arca.fiocruz.br/bitstream/icict/16185/3/atina_elias_etal_IOC_2011.pdf.txt45461e463684bf229ec717fdf659ce30MD53icict/161852018-04-02 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dc.title.pt_BR.fl_str_mv |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
title |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
spellingShingle |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes Abadia, Edgar Mycobacterium tuberculosis Garantia de qualidade Mycobacterium tuberculosis microbead-based spoligotyping Quality Assurance |
title_short |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
title_full |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
title_fullStr |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
title_full_unstemmed |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
title_sort |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes |
author |
Abadia, Edgar |
author_facet |
Abadia, Edgar Zhang, Jian Ritacco, Viviana Kremer, Kristin Ruimy, Raymond Rigouts, Leen Gomes, Harrison Magdinier Elias, Atiná Ribeiro Fauville-Dufaux, Maryse Stoffels, Karolien Rasolofo-Razanamparany, Voahangy Garcia de Viedma, Darío Herranz, Marta Al-Hajoj, Sahal Rastogi, Nalin Garzelli, Carlo Tortoli, Enrico Suffys, Philip N. van Soolingen, Dick Refrégier, Guislaine Sola, Christophe |
author_role |
author |
author2 |
Zhang, Jian Ritacco, Viviana Kremer, Kristin Ruimy, Raymond Rigouts, Leen Gomes, Harrison Magdinier Elias, Atiná Ribeiro Fauville-Dufaux, Maryse Stoffels, Karolien Rasolofo-Razanamparany, Voahangy Garcia de Viedma, Darío Herranz, Marta Al-Hajoj, Sahal Rastogi, Nalin Garzelli, Carlo Tortoli, Enrico Suffys, Philip N. van Soolingen, Dick Refrégier, Guislaine Sola, Christophe |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Abadia, Edgar Zhang, Jian Ritacco, Viviana Kremer, Kristin Ruimy, Raymond Rigouts, Leen Gomes, Harrison Magdinier Elias, Atiná Ribeiro Fauville-Dufaux, Maryse Stoffels, Karolien Rasolofo-Razanamparany, Voahangy Garcia de Viedma, Darío Herranz, Marta Al-Hajoj, Sahal Rastogi, Nalin Garzelli, Carlo Tortoli, Enrico Suffys, Philip N. van Soolingen, Dick Refrégier, Guislaine Sola, Christophe |
dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Garantia de qualidade |
topic |
Mycobacterium tuberculosis Garantia de qualidade Mycobacterium tuberculosis microbead-based spoligotyping Quality Assurance |
dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium tuberculosis microbead-based spoligotyping Quality Assurance |
description |
CNRS Université Paris-Sud 11 Universud, Campus d’Orsay. Institute of Genetics and Microbiology UMR8621. Orsay, France. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2016-10-11T14:01:27Z |
dc.date.available.fl_str_mv |
2016-10-11T14:01:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ABADIA, Edgar. et al. The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes. BMC Infectious Diseases, v.11, n.110, 8p, 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/16185 |
dc.identifier.issn.pt_BR.fl_str_mv |
1471-2334 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2334-11-110 |
identifier_str_mv |
ABADIA, Edgar. et al. The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes. BMC Infectious Diseases, v.11, n.110, 8p, 2011. 1471-2334 10.1186/1471-2334-11-110 |
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https://www.arca.fiocruz.br/handle/icict/16185 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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BioMed Central |
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BioMed Central |
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