Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31412 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Pós-graduação Stricto sensu. Rio de Janeiro, RJ, Brasil. |
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Abrantes, Tuanne RottiMadeira, Maria de FátimaSilva, Denise Amaro daPerié, Carolina dos Santos F. S.Mendes Júnior, Artur Augusto V.Menezes, Rodrigo CaldasSilva, Valmir LaurentinoFigueiredo, Fabiano Borges2019-02-05T11:31:40Z2019-02-05T11:31:40Z2016ABRANTES, Tuanne Rotti et al. Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, n. 3, p. 1-5, 2016.0036-4665https://www.arca.fiocruz.br/handle/icict/3141210.1590/S1678-99462016580031678-9946engUniversidade de São PauloIdentification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Pós-graduação Stricto sensu. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Pós-graduação Stricto sensu. Rio de Janeiro, RJ, Brasil.Veterinária autônoma.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Laboratório de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.After the report of a second case of canine visceral leishmaniasis (CVL) in São Bento da Lagoa, Itaipuaçu, in the municipality of Maricá, Rio de Janeiro State, an epidemiological survey was carried out, through active search, totaling 145 dogs. Indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), and rapid chromatographic immunoassay based on dual-path platform (DPP) were used to perform the serological examinations. The parasitological diagnosis of cutaneous fragments was performed by parasitological culture, histopathology, and immunohistochemistry. In the serological assessment, 21 dogs were seropositive by IFA, 17 by ELISA, and 11 by DPP, with sensitivity of 66.7%, 66.7% and 50%, and specificity of 87.2%, 90.2% and 94%, respectively for each technique. The immunohistochemistry of bone marrow using the cell-block technique presented the best results, with six positive dogs found, three of which tested negative by the other parasitological techniques. Leishmania sp. was isolated by parasitological culture in three dogs. The detection of autochthonous Leishmania infantum in Itaipuaçu, and the high prevalence of seropositive dogs confirm the circulation of this parasite in the study area and alert for the risk of expansion in the State of Rio de Janeiro.Canine visceral leishmaniasisDogsDiagnosisCell-blockinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83100https://www.arca.fiocruz.br/bitstream/icict/31412/1/license.txte0c0e718db411cbd1e3f91252b389ec2MD51ORIGINALve_Abrantes_Tuanne_etal_INI_2016.pdfve_Abrantes_Tuanne_etal_INI_2016.pdfapplication/pdf91866https://www.arca.fiocruz.br/bitstream/icict/31412/2/ve_Abrantes_Tuanne_etal_INI_2016.pdf35d6bcc258bc1d7642295e284ac3d194MD52TEXTve_Abrantes_Tuanne_etal_INI_2016.pdf.txtve_Abrantes_Tuanne_etal_INI_2016.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| title |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| spellingShingle |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation Abrantes, Tuanne Rotti Canine visceral leishmaniasis Dogs Diagnosis Cell-block |
| title_short |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| title_full |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| title_fullStr |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| title_full_unstemmed |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| title_sort |
Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation |
| author |
Abrantes, Tuanne Rotti |
| author_facet |
Abrantes, Tuanne Rotti Madeira, Maria de Fátima Silva, Denise Amaro da Perié, Carolina dos Santos F. S. Mendes Júnior, Artur Augusto V. Menezes, Rodrigo Caldas Silva, Valmir Laurentino Figueiredo, Fabiano Borges |
| author_role |
author |
| author2 |
Madeira, Maria de Fátima Silva, Denise Amaro da Perié, Carolina dos Santos F. S. Mendes Júnior, Artur Augusto V. Menezes, Rodrigo Caldas Silva, Valmir Laurentino Figueiredo, Fabiano Borges |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Abrantes, Tuanne Rotti Madeira, Maria de Fátima Silva, Denise Amaro da Perié, Carolina dos Santos F. S. Mendes Júnior, Artur Augusto V. Menezes, Rodrigo Caldas Silva, Valmir Laurentino Figueiredo, Fabiano Borges |
| dc.subject.en.pt_BR.fl_str_mv |
Canine visceral leishmaniasis Dogs Diagnosis Cell-block |
| topic |
Canine visceral leishmaniasis Dogs Diagnosis Cell-block |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Pós-graduação Stricto sensu. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016 |
| dc.date.accessioned.fl_str_mv |
2019-02-05T11:31:40Z |
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2019-02-05T11:31:40Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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ABRANTES, Tuanne Rotti et al. Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, n. 3, p. 1-5, 2016. |
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https://www.arca.fiocruz.br/handle/icict/31412 |
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0036-4665 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S1678-9946201658003 |
| dc.identifier.eissn.none.fl_str_mv |
1678-9946 |
| identifier_str_mv |
ABRANTES, Tuanne Rotti et al. Identification of canine visceral leishmaniasis in a previously unaffected area by conventional diagnostic techniques and cell-block fixation. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, n. 3, p. 1-5, 2016. 0036-4665 10.1590/S1678-9946201658003 1678-9946 |
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https://www.arca.fiocruz.br/handle/icict/31412 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade de São Paulo |
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Universidade de São Paulo |
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