Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1186/s12936-015-0688-1 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13847 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil. |
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Riccio, Evelyn K. P.Riccio, Lilian R. PrattBianco Júnior, CesareSanchez, VioletteTotino, Paulo R. R.Carvalho, Leonardo J. M.Daniel-Ribeiro, Cláudio Tadeu2016-04-18T15:22:25Z2016-04-18T15:22:25Z2015RICCIO, Evelyn K. P. et al. Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research. Malaria Journal, v.14:166, 17p, 2015.1475-2875https://www.arca.fiocruz.br/handle/icict/1384710.1186/s12936-015-0688-1engBioMed CentralMolecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil / Sanofi Pasteur. Research Department. Lyon, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil.Background: The neotropical, non-human primates (NHP) of the genus Saimiri and Aotus are recommended by the World Health Organization as experimental models for the study of human malaria because these animals can be infected with the same Plasmodium that cause malaria in humans. However, one limitation is the lack of immunological tools to assess the immune response in these models. The present study focuses on the development and comparative use of molecular and immunological methods to evaluate the cellular immune response in Saimiri sciureus. Methods: Blood samples were obtained from nineteen uninfected Saimiri. Peripheral blood mononuclear cells (PBMC) from these animals and splenocytes from one splenectomized animal were cultured for 6, 12, 18, 24, 48, 72 and 96 hrs in the presence of phorbol-12-myristate-13-acetate and ionomycin. The cytokine levels in the supernatant were detected using human and NHP cytometric bead array Th1/Th2 cytokine kits, the Bio-Plex Pro Human Cytokine Th1/Th2 Assay, enzyme-linked immunosorbent assay, enzyme-linked immunospot assays and intracellular cytokine secretion assays. Cytokine gene expression was examined through TaqMan® Gene Expression Real-Time PCR using predesigned human gene-specific primers and probes or primers and probes designed based on published S. sciureus cytokine sequences. Results: The use of five assays based on monoclonal antibodies specific for human cytokines facilitated the detection of IL-2, IL-4 and/or IFN-γ. TaqMan array plates facilitated the detection of 12 of the 28 cytokines assayed. However, only seven cytokines (IL-1A, IL-2, IL-10, IL-12B, IL-17, IFN-β, and TNF) presented relative expression levels of at least 70% of the gene expression observed in human PBMC. The use of primers and probes specific for S. sciureus cytokines facilitated the detection of transcripts that showed relative expression below the threshold of 70%. The most efficient evaluation of cytokine gene expression, in PBMC and splenocytes, was observed after 6–12 hrs of culture, except for LTA in PBMC, whose expression was best analysed after 24 hrs of culture. Conclusions: Real-time PCR facilitates the analysis of a large number of cytokines altered during malaria infection, and this technique is considered the best tool for the evaluation of the cellular immune response in S. sciureus.Saimiri sciureusImmune responseMalariaCytokinesCellular responseCitocinasMaláriaReação em Cadeia da PolimeraseAutoimunidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13847/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALevelyn_riccio_etal_IOC_2015.pdfevelyn_riccio_etal_IOC_2015.pdfapplication/pdf2926549https://www.arca.fiocruz.br/bitstream/icict/13847/2/evelyn_riccio_etal_IOC_2015.pdf28585c1d7875e0220cd46369a69c3e81MD52TEXTevelyn_riccio_etal_IOC_2015.pdf.txtevelyn_riccio_etal_IOC_2015.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| title |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| spellingShingle |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research Riccio, Evelyn K. P. Saimiri sciureus Immune response Malaria Cytokines Cellular response Citocinas Malária Reação em Cadeia da Polimerase Autoimunidade |
| title_short |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| title_full |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| title_fullStr |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| title_full_unstemmed |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| title_sort |
Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research |
| author |
Riccio, Evelyn K. P. |
| author_facet |
Riccio, Evelyn K. P. Riccio, Lilian R. Pratt Bianco Júnior, Cesare Sanchez, Violette Totino, Paulo R. R. Carvalho, Leonardo J. M. Daniel-Ribeiro, Cláudio Tadeu |
| author_role |
author |
| author2 |
Riccio, Lilian R. Pratt Bianco Júnior, Cesare Sanchez, Violette Totino, Paulo R. R. Carvalho, Leonardo J. M. Daniel-Ribeiro, Cláudio Tadeu |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Riccio, Evelyn K. P. Riccio, Lilian R. Pratt Bianco Júnior, Cesare Sanchez, Violette Totino, Paulo R. R. Carvalho, Leonardo J. M. Daniel-Ribeiro, Cláudio Tadeu |
| dc.subject.en.pt_BR.fl_str_mv |
Saimiri sciureus Immune response Malaria Cytokines Cellular response |
| topic |
Saimiri sciureus Immune response Malaria Cytokines Cellular response Citocinas Malária Reação em Cadeia da Polimerase Autoimunidade |
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Citocinas Malária Reação em Cadeia da Polimerase Autoimunidade |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal). Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância Sanitária. Centro de Pesquisa para Malária na Região Extra-Amazônica. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2016-04-18T15:22:25Z |
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2016-04-18T15:22:25Z |
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RICCIO, Evelyn K. P. et al. Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research. Malaria Journal, v.14:166, 17p, 2015. |
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https://www.arca.fiocruz.br/handle/icict/13847 |
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1475-2875 |
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10.1186/s12936-015-0688-1 |
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RICCIO, Evelyn K. P. et al. Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research. Malaria Journal, v.14:166, 17p, 2015. 1475-2875 10.1186/s12936-015-0688-1 |
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BioMed Central |
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BioMed Central |
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