A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Edward Jose de
Data de Publicação: 2016
Outros Autores: Saliba, Juliana Wilke, Oliveira, Diana, Dias, Edelberto Santos, Paz, Gustavo Fontes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.vetpar.2016.02.006
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16268
Resumo: 2021-01-01
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spelling Oliveira, Edward Jose deSaliba, Juliana WilkeOliveira, DianaDias, Edelberto SantosPaz, Gustavo Fontes2016-10-18T13:29:45Z2016-10-18T13:29:45Z2016OLIVEIRA, Edward José de et al. A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis. Vet Parasitol., vol. 221, p. 9-13, 20160304-4017https://www.arca.fiocruz.br/handle/icict/1626810.1016/j.vetpar.2016.02.006engElsevier Scientific Pub. CoLeishmanioseCanine visceral leishmaniasisSerological diagnosisDAT-Canis Control programA prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Pesquisas Clinicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, BrasilThis report describes the stege I/II development of a new direct agglutination test (DAT) for the diagnosis of canine visceral leishmaniasis (CVL) using freeze-dried antigen produced Coomassie blue-stained Leish-mania (Leishmania) infantum promastigotes. In stage I, 16 canine serum samples, collected from eight dogs carrying CVL and eight healthy dogs, were assessed with the DAT using 2-mercaptoethanol (2-ME), N-acetyl-cysteine (NAC), kaolin or NAC plus urea (NAC + U) to improve the assay conditions. Stage II assessed the diagnostic accuracy with 100 serum samples collected from dogs with symptomatic CVL and clin-ically healthy dogs, comparing the four different sample diluents. The CVL-DAT prototype kit showed equivalent performances when 2-ME, NAC or NAC + U were used: 97.1% sensitivity (CI: 83–99.8%), 97% specificity (CI: 88.5–99.5%) and a 97% diagnostic accuracy (CI: 90.8–99.2). With kaolin, a 94.1% sensitivity (CI: 79–99%), 97% specificity (CI: 88.5–99.5%) and 96% diagnostic accuracy were observed (CI: 89.5–98.7), with no statistically significant differences among the four reagents (p = 1.0). The NAC plus urea in sample diluent decreased non-specific agglutination, promoted a better defined sharp-edged blue spot and was thus chosen as a component for the new DAT prototype to diagnose canine VL, designated DAT-Canis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16268/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Oliveira_Edward_A prototype_CPqRR_2016.pdfve_Oliveira_Edward_A prototype_CPqRR_2016.pdfapplication/pdf495335https://www.arca.fiocruz.br/bitstream/icict/16268/2/ve_Oliveira_Edward_A%20prototype_CPqRR_2016.pdf06e9887ee0126fabb84ec99ae9329805MD52TEXTve_Oliveira_Edward_A prototype_CPqRR_2016.pdf.txtve_Oliveira_Edward_A prototype_CPqRR_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
title A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
spellingShingle A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
Oliveira, Edward Jose de
Leishmaniose
Canine visceral leishmaniasis
Serological diagnosis
DAT-Canis Control program
title_short A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
title_full A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
title_fullStr A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
title_full_unstemmed A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
title_sort A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis
author Oliveira, Edward Jose de
author_facet Oliveira, Edward Jose de
Saliba, Juliana Wilke
Oliveira, Diana
Dias, Edelberto Santos
Paz, Gustavo Fontes
author_role author
author2 Saliba, Juliana Wilke
Oliveira, Diana
Dias, Edelberto Santos
Paz, Gustavo Fontes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Edward Jose de
Saliba, Juliana Wilke
Oliveira, Diana
Dias, Edelberto Santos
Paz, Gustavo Fontes
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
topic Leishmaniose
Canine visceral leishmaniasis
Serological diagnosis
DAT-Canis Control program
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Serological diagnosis
DAT-Canis Control program
description 2021-01-01
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-10-18T13:29:45Z
dc.date.available.fl_str_mv 2016-10-18T13:29:45Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Edward José de et al. A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis. Vet Parasitol., vol. 221, p. 9-13, 2016
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16268
dc.identifier.issn.pt_BR.fl_str_mv 0304-4017
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetpar.2016.02.006
identifier_str_mv OLIVEIRA, Edward José de et al. A prototype of the direct agglutination test kit (DAT-Canis) for the serological diagnosis of canine visceral leishmaniasis. Vet Parasitol., vol. 221, p. 9-13, 2016
0304-4017
10.1016/j.vetpar.2016.02.006
url https://www.arca.fiocruz.br/handle/icict/16268
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Scientific Pub. Co
publisher.none.fl_str_mv Elsevier Scientific Pub. Co
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16268/1/license.txt
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