Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP)
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44181 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil. |
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Silva Jr, F. P.Veyl, F. Z.Clos, J.De Simone, Salvatore Giovanni2020-10-25T19:45:24Z2020-10-25T19:45:24Z2002SILVA JR, F. P. et al. Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 3, p. 335-341, Apr. 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4418110.1590/S0074-027620020003000101678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Modelagem molecularProteína de ligação poli-ALeishmania amazonensisBioinformaticsMolecular modelingPoly-A binding proteinLeishmania amazonensisLaPABPBioinformaticsMolecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil.Tropical Medicine Institute. Unit of Leishmania Cloning. Hamburg, Germany.Tropical Medicine Institute. Unit of Leishmania Cloning. Hamburg, Germany.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil.he great expansion in the number of genome sequencing projects has revealed the importance of computational methods to speed up the characterization of unknown genes. These studies have been improved by the use of three dimensional information from the predicted proteins generated by molecular modeling techniques. In this work, we disclose the structure-function relationship of a gene product from Leishmania amazonensis by applying molecular modeling and bioinformatics techniques. The analyzed sequence encodes a 159 aminoacids polypeptide (estimated 18 kDa) and was denoted LaPABP for its high homology with poly-A binding proteins from trypanosomatids. The domain structure, clustering analysis and a three dimensional model of LaPABP, basically obtained by homology modeling on the structure of the human poly-A binding protein, are described. Based on the analysis of the electrostatic potential mapped on the model's surface and conservation of intramolecular contacts responsible for folding stabilization we hypothesize that this protein may have less avidity to RNA than it's L. major counterpart but still account for a significant functional activity in the parasite. The model obtained will help in the design of mutagenesis experiments aimed to elucidate the mechanism of gene expression in trypanosomatids and serve as a starting point for its exploration as a potential source of targets for a rational chemotherapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44181/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSGiovanni_DeSimone_etal_IOC_2002.pdfSGiovanni_DeSimone_etal_IOC_2002.pdfapplication/pdf222142https://www.arca.fiocruz.br/bitstream/icict/44181/2/SGiovanni_DeSimone_etal_IOC_2002.pdf471a6947b403698ee4d2226f44dfd8c2MD52TEXTSGiovanni_DeSimone_etal_IOC_2002.pdf.txtSGiovanni_DeSimone_etal_IOC_2002.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
title |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
spellingShingle |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) Silva Jr, F. P. Modelagem molecular Proteína de ligação poli-A Leishmania amazonensis Bioinformatics Molecular modeling Poly-A binding protein Leishmania amazonensis LaPABP Bioinformatics |
title_short |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
title_full |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
title_fullStr |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
title_full_unstemmed |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
title_sort |
Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) |
author |
Silva Jr, F. P. |
author_facet |
Silva Jr, F. P. Veyl, F. Z. Clos, J. De Simone, Salvatore Giovanni |
author_role |
author |
author2 |
Veyl, F. Z. Clos, J. De Simone, Salvatore Giovanni |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva Jr, F. P. Veyl, F. Z. Clos, J. De Simone, Salvatore Giovanni |
dc.subject.other.pt_BR.fl_str_mv |
Modelagem molecular Proteína de ligação poli-A Leishmania amazonensis Bioinformatics |
topic |
Modelagem molecular Proteína de ligação poli-A Leishmania amazonensis Bioinformatics Molecular modeling Poly-A binding protein Leishmania amazonensis LaPABP Bioinformatics |
dc.subject.en.pt_BR.fl_str_mv |
Molecular modeling Poly-A binding protein Leishmania amazonensis LaPABP Bioinformatics |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2020-10-25T19:45:24Z |
dc.date.available.fl_str_mv |
2020-10-25T19:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA JR, F. P. et al. Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 3, p. 335-341, Apr. 2002. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44181 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762002000300010 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
SILVA JR, F. P. et al. Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 3, p. 335-341, Apr. 2002. 0074-0276 10.1590/S0074-02762002000300010 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/44181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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