Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25931 |
Resumo: | Fundação Butantan and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP – 2012/23124-4 and FAPESP – 2010/18486-9). |
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Fernandes, Rafaela SachettoFernandes, Luis Guilherme VirgilioGodoy, Andre Schutzer deMiyasato, Patrícia AokiNakano, ElianaFarias, Leonardo PaivaNascimento, Ana Lucia Tabet Oller doLeite, Luciana Cezar de Cerqueira2018-04-18T17:23:51Z2018-04-18T17:23:51Z2018FERNANDES, R. S. et al. Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection. Molecular and Biochemical Parasitology, v. 221, p. 23–31, 2018.0166-6851https://www.arca.fiocruz.br/handle/icict/2593110.1016/j.molbiopara.2018.02.003Fundação Butantan and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP – 2012/23124-4 and FAPESP – 2010/18486-9).Instituto Butantan. Laboratorio de Desenvolvimento de Vacinas. São Paulo, SP, Brasil / Universidade de São Paulo. Pós-Graduação Interunidades em Biotecnologia. São Paulo, SP, BrasilInstituto Butantan. Laboratorio de Desenvolvimento de Vacinas. São Paulo, SP, Brasil / Universidade de São Paulo. Pós-Graduação Interunidades em Biotecnologia. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, BrasilInstituto Butantan. Laboratório de Parasitologia. São Paulo, SP, BrasilInstituto Butantan. Laboratório de Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilInstituto Butantan. Laboratorio de Desenvolvimento de Vacinas. São Paulo, SP, BrasilInstituto Butantan. Laboratorio de Desenvolvimento de Vacinas. São Paulo, SP, BrasilSchistosomiasis is a neglected tropical disease caused by trematodes of the genus Schistosoma which have a complex life cycle characterized by an asexual multiplication phase in the snail intermediate host and a sexual reproduction phase in the mammalian definitive host. The initial steps of the human host infection involve the secretion of proteins contained in the acetabular glands of cercariae that promote parasite adhesion and proteolysis of the skin layers. Herein, we performed a functional analysis of SmVAL18, identified as one of the three SCP/TAPS proteins constituent of cercarial secretions. We evaluated the SmVAL18 binding to immobilized macromolecules of the extracellular matrix (ECM) and to plasma components. Recombinant protein, expressed in E. coli, was found to maintain an ordered secondary structure typical of the SCP/TAPS domain after purification. Expression of native SmVAL18 protein was verified to be restricted to cercariae and 3-h schistosomula stages; furthermore, the protein was observed in the corresponding secretions, confirming that SmVAL18 is secreted during the first 3 h of in vitro culture. rSmVAL18 was able to interact specifically with plasminogen (PLG) and enhance its conversion into plasmin in the presence of the urokinase-type plasminogen activator (uPA). Protein homology modelling suggested that the PLG-rSmVAL18 interaction was mediated by lysine residues of the protein. This was supported by in vitro data using the lysine analogue, 6-aminocaproic acid (ACA), which abolished the interaction. Finally, our results showed that both cercariae and 3-h schistosomula, as well as their corresponding secretions, exhibited the capacity to bind PLG and enhance its conversion into plasmin in vitro in the same way as observed for the recombinant protein. In conclusion, our findings show that SmVAL18 is a novel PLG-binding protein secreted during the early stages of the mammalian-host infection.engElsevierEsquistossomoseSCP/TAPSSmVAL18PlasminogênioProteína PLGSchistosomiasisSCP/TAPSSmVAL18PlasminogenPLG-binding proteinSchistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25931/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFernandes R Schistosoma mansoni venom allergen-like...pdfFernandes R Schistosoma mansoni venom allergen-like...pdfapplication/pdf1525994https://www.arca.fiocruz.br/bitstream/icict/25931/2/Fernandes%20R%20Schistosoma%20mansoni%20venom%20allergen-like...pdfa5b5a8a1de73c2b0ded6e393837bc259MD52TEXTFernandes R Schistosoma mansoni venom allergen-like...pdf.txtFernandes R Schistosoma mansoni venom allergen-like...pdf.txtExtracted texttext/plain48135https://www.arca.fiocruz.br/bitstream/icict/25931/3/Fernandes%20R%20Schistosoma%20mansoni%20venom%20allergen-like...pdf.txtf70c78b985190caafbc55ca9ec339132MD53icict/259312023-03-15 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dc.title.pt_BR.fl_str_mv |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
title |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
spellingShingle |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection Fernandes, Rafaela Sachetto Esquistossomose SCP/TAPS SmVAL18 Plasminogênio Proteína PLG Schistosomiasis SCP/TAPS SmVAL18 Plasminogen PLG-binding protein |
title_short |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
title_full |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
title_fullStr |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
title_full_unstemmed |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
title_sort |
Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection |
author |
Fernandes, Rafaela Sachetto |
author_facet |
Fernandes, Rafaela Sachetto Fernandes, Luis Guilherme Virgilio Godoy, Andre Schutzer de Miyasato, Patrícia Aoki Nakano, Eliana Farias, Leonardo Paiva Nascimento, Ana Lucia Tabet Oller do Leite, Luciana Cezar de Cerqueira |
author_role |
author |
author2 |
Fernandes, Luis Guilherme Virgilio Godoy, Andre Schutzer de Miyasato, Patrícia Aoki Nakano, Eliana Farias, Leonardo Paiva Nascimento, Ana Lucia Tabet Oller do Leite, Luciana Cezar de Cerqueira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Rafaela Sachetto Fernandes, Luis Guilherme Virgilio Godoy, Andre Schutzer de Miyasato, Patrícia Aoki Nakano, Eliana Farias, Leonardo Paiva Nascimento, Ana Lucia Tabet Oller do Leite, Luciana Cezar de Cerqueira |
dc.subject.other.pt_BR.fl_str_mv |
Esquistossomose SCP/TAPS SmVAL18 Plasminogênio Proteína PLG |
topic |
Esquistossomose SCP/TAPS SmVAL18 Plasminogênio Proteína PLG Schistosomiasis SCP/TAPS SmVAL18 Plasminogen PLG-binding protein |
dc.subject.en.pt_BR.fl_str_mv |
Schistosomiasis SCP/TAPS SmVAL18 Plasminogen PLG-binding protein |
description |
Fundação Butantan and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP – 2012/23124-4 and FAPESP – 2010/18486-9). |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-18T17:23:51Z |
dc.date.available.fl_str_mv |
2018-04-18T17:23:51Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, R. S. et al. Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection. Molecular and Biochemical Parasitology, v. 221, p. 23–31, 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/25931 |
dc.identifier.issn.pt_BR.fl_str_mv |
0166-6851 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.molbiopara.2018.02.003 |
identifier_str_mv |
FERNANDES, R. S. et al. Schistosoma mansoni venom allergen-like protein 18 (SmVAL18) is a plasminogen-binding protein secreted during the early stages of mammalian-host infection. Molecular and Biochemical Parasitology, v. 221, p. 23–31, 2018. 0166-6851 10.1016/j.molbiopara.2018.02.003 |
url |
https://www.arca.fiocruz.br/handle/icict/25931 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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Repositório Institucional da FIOCRUZ (ARCA) |
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