Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation

Detalhes bibliográficos
Autor(a) principal: Latrofa, Maria Stefania
Data de Publicação: 2016
Outros Autores: Dantas-Torres, Filipe, Caprariis, Donato de, Cantacessi, Cinzia, Capelli, Gioia, Lia, Riccardo Paolo, Breitschwerdt, Edward B., Otranto, Domenico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19397
Resumo: Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.
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spelling Latrofa, Maria StefaniaDantas-Torres, FilipeCaprariis, Donato deCantacessi, CinziaCapelli, GioiaLia, Riccardo PaoloBreitschwerdt, Edward B.Otranto, Domenico2017-06-16T14:15:44Z2017-06-16T14:15:44Z2016LATROFA, M. S. et al. Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation. Parasites & Vectors, v. 9, n. 1, dez. 2016.1756-3305https://www.arca.fiocruz.br/handle/icict/1939710.1186/s13071-016-1545-yengVertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversità degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.1 Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.University of Cambridge. Department of Veterinary Medicine. Cambridge, UK.Istituto Zooprofilattico Sperimentale delle Venezie. Legnaro, Padova, Italy.Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.North Carolina State University. College of Veterinary Medicine. Center for Comparative Medicine. Intracellular Pathogens Research Laboratory. Raleigh, NC, USA.Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.Background: Leishmania infantum is a canine zoonotic vector-borne protozoan pathogen transmitted by phlebotomine sand flies, whereas Anaplasma platys is a bacterium most likely transmitted by ticks. While vertical transmission of L. infantum from pregnant bitches to their offspring has been documented, thus far no studies have explored the possibility of vertical transmission of A. platys in dogs. This study investigated the occurrence of vertical transmission of L. infantum and A. platys in sheltered dogs during the first half of gestation, in an area of southern Italy characterised by a high incidence of infection by both pathogens. Methods: The study population included 20 bitches (n = 10 pregnant, at 25–35 days of pregnancy; n = 10 nonpregnant), all subjected to ovariohysterectomy, which were examined for the presence of L. infantum and A. platys via cytological screening of bone marrow and whole blood samples. Infection by L. infantum and A. platys was also tested by immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR) targeting both pathogens. Selected tissue samples (n = 210) collected during surgical procedures from bitches and foetuses (n = 20) were assessed for the presence of L. infantum and A. platys by qPCR targeting a fragment of the kinetoplast minicircle DNA (kDNA) and the 16S rRNA gene, respectively. Results: Leishmania infantum DNA was not amplified from either uteri or ovaries from pregnant bitches or foetal tissue samples, whereas a subset of ovarian (n = 2) and uterine (n = 4) tissue samples from non-pregnant bitches were infected, with parasite loads of up to 3.09 × 10 and 7.51 parasite/PCR reaction, respectively. Conversely, uterine (n = 10) and ovarian (n = 8) tissues from both pregnant and non-pregnant bitches, together with a subset (n = 5) of foetal tissue samples were qPCR positive for A. platys. Leishmania infantum and A. platys nucleic acids were amplified from two uteri from nonpregnant bitches, with parasite loads of up to 2.32 × 10-3 and 2.05 parasite/per PCR reaction, respectively. Conclusions: Results from this study suggest that, in contrast to L. infantum, A. platys can be transmitted from pregnant dogs to their offspring during the first half of gestation. This hypothesis remains to be verified, for instance via direct observation of parasites in postpartum foetal tissuesAnaplasmose / transmissãoDoenças do Cão / transmissãoLeishmaniose Visceral / veterináriaDoença Infecciosa Transmissão , Vertical / veterinárioComplicações na Gravidez Infecciosa / veterináriaLeishmania infantum / fisiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19397/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27161003 2016 lat-ver.oa.pdf27161003 2016 lat-ver.oa.pdfapplication/pdf404450https://www.arca.fiocruz.br/bitstream/icict/19397/2/27161003%202016%20lat-ver.oa.pdf2bf55a6b4096a129376cbfcd6a99f72bMD52TEXT27161003 2016 lat-ver.oa.pdf.txt27161003 2016 lat-ver.oa.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
title Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
spellingShingle Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
Latrofa, Maria Stefania
Anaplasmose / transmissão
Doenças do Cão / transmissão
Leishmaniose Visceral / veterinária
Doença Infecciosa Transmissão , Vertical / veterinário
Complicações na Gravidez Infecciosa / veterinária
Leishmania infantum / fisiologia
title_short Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
title_full Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
title_fullStr Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
title_full_unstemmed Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
title_sort Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation
author Latrofa, Maria Stefania
author_facet Latrofa, Maria Stefania
Dantas-Torres, Filipe
Caprariis, Donato de
Cantacessi, Cinzia
Capelli, Gioia
Lia, Riccardo Paolo
Breitschwerdt, Edward B.
Otranto, Domenico
author_role author
author2 Dantas-Torres, Filipe
Caprariis, Donato de
Cantacessi, Cinzia
Capelli, Gioia
Lia, Riccardo Paolo
Breitschwerdt, Edward B.
Otranto, Domenico
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Latrofa, Maria Stefania
Dantas-Torres, Filipe
Caprariis, Donato de
Cantacessi, Cinzia
Capelli, Gioia
Lia, Riccardo Paolo
Breitschwerdt, Edward B.
Otranto, Domenico
dc.subject.decs.none.fl_str_mv Anaplasmose / transmissão
Doenças do Cão / transmissão
Leishmaniose Visceral / veterinária
Doença Infecciosa Transmissão , Vertical / veterinário
Complicações na Gravidez Infecciosa / veterinária
Leishmania infantum / fisiologia
topic Anaplasmose / transmissão
Doenças do Cão / transmissão
Leishmaniose Visceral / veterinária
Doença Infecciosa Transmissão , Vertical / veterinário
Complicações na Gravidez Infecciosa / veterinária
Leishmania infantum / fisiologia
description Università degli Studi di Bari. Dipartimento di Medicina Veterinaria. Valenzano, Bari, Italy.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-06-16T14:15:44Z
dc.date.available.fl_str_mv 2017-06-16T14:15:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv LATROFA, M. S. et al. Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation. Parasites & Vectors, v. 9, n. 1, dez. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19397
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s13071-016-1545-y
identifier_str_mv LATROFA, M. S. et al. Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation. Parasites & Vectors, v. 9, n. 1, dez. 2016.
1756-3305
10.1186/s13071-016-1545-y
url https://www.arca.fiocruz.br/handle/icict/19397
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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