Molecular typing of Campylobacter jejuni strains: comparison among four different techniques

Detalhes bibliográficos
Autor(a) principal: Frazão, Miliane Rodrigues
Data de Publicação: 2020
Outros Autores: Souza, Roberto Antonio de, Medeiros, Marta Inês Cazentini, Duque, Sheila da Silva, Cao, Guojie, Alard, Marc William, Falcão, Juliana Pfrimer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45718
Resumo: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
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spelling Frazão, Miliane RodriguesSouza, Roberto Antonio deMedeiros, Marta Inês CazentiniDuque, Sheila da SilvaCao, GuojieAlard, Marc WilliamFalcão, Juliana Pfrimer2021-01-15T18:04:16Z2021-01-15T18:04:16Z2020FRAZÃO, Miliane Rodrigues et al. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 515, p. 519-525, Dec. 2020.1517-8382https://www.arca.fiocruz.br/handle/icict/4571810.1007/s42770-019-00218-81678-4405engSociedade Brasileira de MicrobiologiaCampylobacter jejuniCRISPR-HRMAEletroforese de gel de campo pulsadoEpidemiologia molecularCampylobacter jejuniPulsed field gel electrophoresisflaA-SVRsequencingCRISPR-HRMAMLSTMolecular epidemiologyMolecular typing of Campylobacter jejuni strains: comparison among four different techniquesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Instituto Adolfo Lutz de Ribeirão Preto. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Division of Microbiology, Office of Regular Science, Center for Food Safety and Applied Nutrition. U.S. Food and Drug Administration. College Park, MD, USA.Division of Microbiology, Office of Regular Science, Center for Food Safety and Applied Nutrition. U.S. Food and Drug Administration. College Park, MD, USA.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.This study compared the ability of pulsed-field gel electrophoresis (PFGE), flaA small variable region (SVR) sequencing, analysis of the clustered regularly interspaced short palindromic repeats locus by high resolution melting analysis (CRISPRHRMA), and multilocus sequence typing (MLST) for typing 111 Campylobacter jejuni strains isolated from diverse sources during 20 years in Brazil. For this, we used previous results obtained by PFGE and flaA-SVR sequencing from our research group and performed CRISPR-HRMA and MLST typing for the first time. Furthermore, the discrimination index (DI) of each method was accessed. The DI for PFGE, flaA-SVR sequencing, CRISPR-HRMA, and MLST was 0.980, 0.932, 0.868, and 0.931, respectively. By PFGE and flaA-SVR sequencing, some strains from clinical and non-clinical sources and from humans and animals presented ≥ 80% similarity. Similarly, some strains from different origins presented the same ST and CRISPR-HRMA types. In conclusion, despite the different DI values, all assays provided the same epidemiological information suggesting that a potential transmission may have occurred between C. jejuni from clinical and non-clinical sources and from animals and humans in Brazil. Furthermore it was demonstrated the suitability of PFGE that should be used preferably together with MLST and/or flaA-SVR sequencing for typing C. jejuni strains.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45718/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSheilaSilva_Duque_etal_IOC_2020.pdfSheilaSilva_Duque_etal_IOC_2020.pdfapplication/pdf271167https://www.arca.fiocruz.br/bitstream/icict/45718/2/SheilaSilva_Duque_etal_IOC_2020.pdf898679d9b37f5d6c802eb23637f33f29MD52TEXTSheilaSilva_Duque_etal_IOC_2020.pdf.txtSheilaSilva_Duque_etal_IOC_2020.pdf.txtExtracted texttext/plain32495https://www.arca.fiocruz.br/bitstream/icict/45718/3/SheilaSilva_Duque_etal_IOC_2020.pdf.txt8fa4a3f619694ee79de7fd3bf9b4b999MD53icict/457182021-01-16 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dc.title.pt_BR.fl_str_mv Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
title Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
spellingShingle Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
Frazão, Miliane Rodrigues
Campylobacter jejuni
CRISPR-HRMA
Eletroforese de gel de campo pulsado
Epidemiologia molecular
Campylobacter jejuni
Pulsed field gel electrophoresis
flaA-SVRsequencing
CRISPR-HRMA
MLST
Molecular epidemiology
title_short Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
title_full Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
title_fullStr Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
title_full_unstemmed Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
title_sort Molecular typing of Campylobacter jejuni strains: comparison among four different techniques
author Frazão, Miliane Rodrigues
author_facet Frazão, Miliane Rodrigues
Souza, Roberto Antonio de
Medeiros, Marta Inês Cazentini
Duque, Sheila da Silva
Cao, Guojie
Alard, Marc William
Falcão, Juliana Pfrimer
author_role author
author2 Souza, Roberto Antonio de
Medeiros, Marta Inês Cazentini
Duque, Sheila da Silva
Cao, Guojie
Alard, Marc William
Falcão, Juliana Pfrimer
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Frazão, Miliane Rodrigues
Souza, Roberto Antonio de
Medeiros, Marta Inês Cazentini
Duque, Sheila da Silva
Cao, Guojie
Alard, Marc William
Falcão, Juliana Pfrimer
dc.subject.other.pt_BR.fl_str_mv Campylobacter jejuni
CRISPR-HRMA
Eletroforese de gel de campo pulsado
Epidemiologia molecular
topic Campylobacter jejuni
CRISPR-HRMA
Eletroforese de gel de campo pulsado
Epidemiologia molecular
Campylobacter jejuni
Pulsed field gel electrophoresis
flaA-SVRsequencing
CRISPR-HRMA
MLST
Molecular epidemiology
dc.subject.en.pt_BR.fl_str_mv Campylobacter jejuni
Pulsed field gel electrophoresis
flaA-SVRsequencing
CRISPR-HRMA
MLST
Molecular epidemiology
description Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-15T18:04:16Z
dc.date.available.fl_str_mv 2021-01-15T18:04:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRAZÃO, Miliane Rodrigues et al. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 515, p. 519-525, Dec. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45718
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.none.fl_str_mv 10.1007/s42770-019-00218-8
dc.identifier.eissn.none.fl_str_mv 1678-4405
identifier_str_mv FRAZÃO, Miliane Rodrigues et al. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 515, p. 519-525, Dec. 2020.
1517-8382
10.1007/s42770-019-00218-8
1678-4405
url https://www.arca.fiocruz.br/handle/icict/45718
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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