Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping

Detalhes bibliográficos
Autor(a) principal: Rebelo, Maria C.
Data de Publicação: 2006
Outros Autores: Boente, Renata F., Matos, Juliana de A., Hofer, Cristina B., Barroso, David E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39617
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil / Instituto Estadual de Infectologia São Sebastião. Rio de Janeiro, RJ, Brasil.
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spelling Rebelo, Maria C.Boente, Renata F.Matos, Juliana de A.Hofer, Cristina B.Barroso, David E.2020-01-30T11:13:59Z2020-01-30T11:13:59Z2006REBELO, Maria C. et al. Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 809-813, Nov. 2006.0074-0206https://www.arca.fiocruz.br/handle/icict/3961710.1590/S0074-027620060007000171678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzNeisseria meningitidisDoença meningocócicaReação em cadeia da polimeraseGenogrupoNeisseria meningitidisMeningococcal diseasePolymerase chain reactionGenogroupAssessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogroupinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil / Instituto Estadual de Infectologia São Sebastião. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil.Immediate prevention of meningococcal disease relies in part on the prompt treatment with antibiotics of household and other close contacts of cases; however intervention with effective vaccination relies on identification of serogroup-causing strains. Parenteral antibiotic for patient with suspected meningococcal disease before hospital admission is currently recommended. Laboratory standard methods are hindered by failure to detect bacteria by this medical approach to improve patient prognosis. We assessed two polymerase chain reaction (PCR) assays to detect (crgA) and define the serogroups (siaD, orf-2, and ctrA) of Neisseria meningitidis in 120 cerebrospinal fluid (CSF) samples from positive cases (culture or antigen detection or direct smear). The PCR sensitivity for the identification of N. meningitidis was 100% (95% confidence interval, CI, 96-100%) compared to a sensitivity of 46% for culture (95% CI 37-55%), 61% for latex agglutination test (95% CI 52-70%), and 68% for Gram stain (95% CI 59-76%); PCR specificity was 97% (95% CI 82-100%). PCR correctly identified the serogroups A, B, C, W135, Y, and X in CSF samples with a sensitivity of 88% (95% CI 80-93%); the primer sets were 100% specific. The introduction of PCR-based assays shall increase laboratory confirmed cases, consequently enhancing surveillance of meningococcal disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39617/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaCRebello-JulianaMatos_etal_IOC_2006.pdfMariaCRebello-JulianaMatos_etal_IOC_2006.pdfapplication/pdf410134https://www.arca.fiocruz.br/bitstream/icict/39617/2/MariaCRebello-JulianaMatos_etal_IOC_2006.pdf1360fe4fa61b50c6f1082319abf9cfc0MD52TEXTMariaCRebello-JulianaMatos_etal_IOC_2006.pdf.txtMariaCRebello-JulianaMatos_etal_IOC_2006.pdf.txtExtracted texttext/plain26104https://www.arca.fiocruz.br/bitstream/icict/39617/3/MariaCRebello-JulianaMatos_etal_IOC_2006.pdf.txt16ec508e7f487a3021a664b020998a97MD53icict/396172020-01-31 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dc.title.pt_BR.fl_str_mv Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
title Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
spellingShingle Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
Rebelo, Maria C.
Neisseria meningitidis
Doença meningocócica
Reação em cadeia da polimerase
Genogrupo
Neisseria meningitidis
Meningococcal disease
Polymerase chain reaction
Genogroup
title_short Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
title_full Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
title_fullStr Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
title_full_unstemmed Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
title_sort Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
author Rebelo, Maria C.
author_facet Rebelo, Maria C.
Boente, Renata F.
Matos, Juliana de A.
Hofer, Cristina B.
Barroso, David E.
author_role author
author2 Boente, Renata F.
Matos, Juliana de A.
Hofer, Cristina B.
Barroso, David E.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rebelo, Maria C.
Boente, Renata F.
Matos, Juliana de A.
Hofer, Cristina B.
Barroso, David E.
dc.subject.other.pt_BR.fl_str_mv Neisseria meningitidis
Doença meningocócica
Reação em cadeia da polimerase
Genogrupo
topic Neisseria meningitidis
Doença meningocócica
Reação em cadeia da polimerase
Genogrupo
Neisseria meningitidis
Meningococcal disease
Polymerase chain reaction
Genogroup
dc.subject.en.pt_BR.fl_str_mv Neisseria meningitidis
Meningococcal disease
Polymerase chain reaction
Genogroup
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil / Instituto Estadual de Infectologia São Sebastião. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2020-01-30T11:13:59Z
dc.date.available.fl_str_mv 2020-01-30T11:13:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REBELO, Maria C. et al. Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 809-813, Nov. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39617
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000700017
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv REBELO, Maria C. et al. Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 809-813, Nov. 2006.
0074-0206
10.1590/S0074-02762006000700017
1678-8060
url https://www.arca.fiocruz.br/handle/icict/39617
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/39617/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/39617/2/MariaCRebello-JulianaMatos_etal_IOC_2006.pdf
https://www.arca.fiocruz.br/bitstream/icict/39617/3/MariaCRebello-JulianaMatos_etal_IOC_2006.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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